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The characterization of obese polycystic ovary syndrome rat model suitable for exercise intervention.

Wu C, Lin F, Qiu S, Jiang Z - PLoS ONE (2014)

Bottom Line: Body weight, Lee index, FINS increased significantly in PF rat model.Serum levels of E2 and T were significantly higher in PF and PO than in CO.Ovary organ index and ovarian areas were significant lower in PF than in CO.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

ABSTRACT

Objective: To develop a new polycystic ovary syndrome (PCOS) rat model suitable for exercise intervention.

Method: Thirty six rats were randomly divided into three experimental groups: PCOS rats with high-fat diet (PF, n = 24), PCOS rats with ordinary diet (PO, n = 6), and control rats with ordinary diet (CO, n = 6). Two kinds of PCOS rat model were made by adjustment diet structure and testosterone injection for 28 days. After a successful animal model, PF model rats were randomly assigned to three groups: exercise with a continuation of high-fat diet (PF-EF, n = 6), sedentary with a continuation of high-fat diet (PF-SF, n = 6), exercise with an ordinary diet (PF-EO, n = 6). Fasting blood glucose (FBG) and insulin (FINS), estrogen (E2), progesterone (P), and testosterone (T) in serum were determined by RIA, and ovarian morphology was evaluated by Image-Pro plus 6.0.

Results: Body weight, Lee index, FINS increased significantly in PF rat model. Serum levels of E2 and T were significantly higher in PF and PO than in CO. Ovary organ index and ovarian areas were significant lower in PF than in CO. After intervention for 2 weeks, the levels of 1 h postprandial blood glucose (PBG1), 2 h postprandial blood glucose (PBG2), FINS and the serum levels of T decreased significantly in PF-EF rats and PF-EO rats. The ratio of FBG/FINS was significant higher in PF-EO rats than in PF-SF rats. Ovarian morphology showed that the numbers of preantral follicles and atretic follicles decreased significantly, and the numbers of antral follicles and corpora lutea increased significantly in the rats of PF-EF and PF-EO.

Conclusion: By combination of high-fat diet and testosterone injection, the obese PCOS rat model is conformable with the lifestyle habits of fatty foods and insufficient exercise, and has metabolic and reproductive characteristics of human PCOS. This model can be applied to study exercise intervention.

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The experiment protocol.O: ordinary diet; F: high fat diet; T: testosterone propionate; CO: control group with ordinary diet; PO: PCOS model with ordinary diet; PF: PCOS model with high-fat diet; S: no exercise; E: exercise; PF-SF: sedentary with high-fat diet in PF rats; PF-EF: exercise with high-fat diet in PF rats; PF-EO: exercise with ordinary diet in PF rats.
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pone-0099155-g001: The experiment protocol.O: ordinary diet; F: high fat diet; T: testosterone propionate; CO: control group with ordinary diet; PO: PCOS model with ordinary diet; PF: PCOS model with high-fat diet; S: no exercise; E: exercise; PF-SF: sedentary with high-fat diet in PF rats; PF-EF: exercise with high-fat diet in PF rats; PF-EO: exercise with ordinary diet in PF rats.

Mentions: All rats were randomly divided into three experimental groups (Fig. 1): PCOS model with high-fat diet (PF, n = 24), PCOS model with ordinary diet (PO, n = 6), and control group with ordinary diet (CO, n = 6). PF rats were fed with high-fat diet for 28 days. CO rats were fed with ordinary diet. High-fat diet composed of 22% fat, 48% carbohydrate, and 20%protein with total calorific value 44.3KJ/Kg [23] and ordinary diet composed of 5% fat, 53% carbohydrate, 23% protein, with total calorific value 25KJ/Kg were ordered in Shanghai SLC Co. (Shanghai, China). At the same time, these rats in PF and PO were subcutaneously injected daily at 8:00 for 28 days with testosterone propionate of 1 mg/100 g body weight dissolved in tea oil [11]. The rats in CO were injected daily with the same volume of tea oil alone. At the day after the end of 28 days of injection, total 18 rats from PF, PO and CO with 6 rats in each group were sacrificed by cervical dislocation.


The characterization of obese polycystic ovary syndrome rat model suitable for exercise intervention.

Wu C, Lin F, Qiu S, Jiang Z - PLoS ONE (2014)

The experiment protocol.O: ordinary diet; F: high fat diet; T: testosterone propionate; CO: control group with ordinary diet; PO: PCOS model with ordinary diet; PF: PCOS model with high-fat diet; S: no exercise; E: exercise; PF-SF: sedentary with high-fat diet in PF rats; PF-EF: exercise with high-fat diet in PF rats; PF-EO: exercise with ordinary diet in PF rats.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4048306&req=5

pone-0099155-g001: The experiment protocol.O: ordinary diet; F: high fat diet; T: testosterone propionate; CO: control group with ordinary diet; PO: PCOS model with ordinary diet; PF: PCOS model with high-fat diet; S: no exercise; E: exercise; PF-SF: sedentary with high-fat diet in PF rats; PF-EF: exercise with high-fat diet in PF rats; PF-EO: exercise with ordinary diet in PF rats.
Mentions: All rats were randomly divided into three experimental groups (Fig. 1): PCOS model with high-fat diet (PF, n = 24), PCOS model with ordinary diet (PO, n = 6), and control group with ordinary diet (CO, n = 6). PF rats were fed with high-fat diet for 28 days. CO rats were fed with ordinary diet. High-fat diet composed of 22% fat, 48% carbohydrate, and 20%protein with total calorific value 44.3KJ/Kg [23] and ordinary diet composed of 5% fat, 53% carbohydrate, 23% protein, with total calorific value 25KJ/Kg were ordered in Shanghai SLC Co. (Shanghai, China). At the same time, these rats in PF and PO were subcutaneously injected daily at 8:00 for 28 days with testosterone propionate of 1 mg/100 g body weight dissolved in tea oil [11]. The rats in CO were injected daily with the same volume of tea oil alone. At the day after the end of 28 days of injection, total 18 rats from PF, PO and CO with 6 rats in each group were sacrificed by cervical dislocation.

Bottom Line: Body weight, Lee index, FINS increased significantly in PF rat model.Serum levels of E2 and T were significantly higher in PF and PO than in CO.Ovary organ index and ovarian areas were significant lower in PF than in CO.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

ABSTRACT

Objective: To develop a new polycystic ovary syndrome (PCOS) rat model suitable for exercise intervention.

Method: Thirty six rats were randomly divided into three experimental groups: PCOS rats with high-fat diet (PF, n = 24), PCOS rats with ordinary diet (PO, n = 6), and control rats with ordinary diet (CO, n = 6). Two kinds of PCOS rat model were made by adjustment diet structure and testosterone injection for 28 days. After a successful animal model, PF model rats were randomly assigned to three groups: exercise with a continuation of high-fat diet (PF-EF, n = 6), sedentary with a continuation of high-fat diet (PF-SF, n = 6), exercise with an ordinary diet (PF-EO, n = 6). Fasting blood glucose (FBG) and insulin (FINS), estrogen (E2), progesterone (P), and testosterone (T) in serum were determined by RIA, and ovarian morphology was evaluated by Image-Pro plus 6.0.

Results: Body weight, Lee index, FINS increased significantly in PF rat model. Serum levels of E2 and T were significantly higher in PF and PO than in CO. Ovary organ index and ovarian areas were significant lower in PF than in CO. After intervention for 2 weeks, the levels of 1 h postprandial blood glucose (PBG1), 2 h postprandial blood glucose (PBG2), FINS and the serum levels of T decreased significantly in PF-EF rats and PF-EO rats. The ratio of FBG/FINS was significant higher in PF-EO rats than in PF-SF rats. Ovarian morphology showed that the numbers of preantral follicles and atretic follicles decreased significantly, and the numbers of antral follicles and corpora lutea increased significantly in the rats of PF-EF and PF-EO.

Conclusion: By combination of high-fat diet and testosterone injection, the obese PCOS rat model is conformable with the lifestyle habits of fatty foods and insufficient exercise, and has metabolic and reproductive characteristics of human PCOS. This model can be applied to study exercise intervention.

Show MeSH
Related in: MedlinePlus