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Correction: A Bioartificial Renal Tubule Device Embedding Human Renal Stem/Progenitor Cells

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Polarization of ARPCs following FSS in the chip.(A) Cell model of proximal tubule cells with transporters. The scheme shows the AQP2 transporter localized in the apical membrane and the Na+K+ATPase present in the basolateral membrane. (B-J) Cellular arrangement of ARPCs grown into the microfluidic device after 6 hs of FSS at 0.2 dyn/cm2. Two different chips were stained in (B-E) and in (G-J), respectively. Immunofluorescence images of stained DNA (DAPI, blue) (B, G), actin (TRITC-phalloidin, red) (C, H), AQP2 (FITC-anti goat, green) (D) and Na+K+ATPase pump (I). X-Z section confocal images for AQP2 (apical marker protein) (E) and Na+K+ATPase pump (basolateral marker protein) (J) following FSS in the chip. Scale bars  =  100 µm. The section along the longitudinal axis of the microchannel is indicated by the white line in D and I, respectively. X-Z section confocal images were also collected for AQP2 (F) and Na+K+ATPase pump (K) in statically cultured ARPCs. Scale bars  =  15 µm.
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pone-0100141-g002: Polarization of ARPCs following FSS in the chip.(A) Cell model of proximal tubule cells with transporters. The scheme shows the AQP2 transporter localized in the apical membrane and the Na+K+ATPase present in the basolateral membrane. (B-J) Cellular arrangement of ARPCs grown into the microfluidic device after 6 hs of FSS at 0.2 dyn/cm2. Two different chips were stained in (B-E) and in (G-J), respectively. Immunofluorescence images of stained DNA (DAPI, blue) (B, G), actin (TRITC-phalloidin, red) (C, H), AQP2 (FITC-anti goat, green) (D) and Na+K+ATPase pump (I). X-Z section confocal images for AQP2 (apical marker protein) (E) and Na+K+ATPase pump (basolateral marker protein) (J) following FSS in the chip. Scale bars  =  100 µm. The section along the longitudinal axis of the microchannel is indicated by the white line in D and I, respectively. X-Z section confocal images were also collected for AQP2 (F) and Na+K+ATPase pump (K) in statically cultured ARPCs. Scale bars  =  15 µm.

Mentions: The figure legends for Figure 2 and 5 are incorrect. There are incorrect values presented for the scale bars in the legends. Please see these figures and their corrected legends below.


Correction: A Bioartificial Renal Tubule Device Embedding Human Renal Stem/Progenitor Cells
Polarization of ARPCs following FSS in the chip.(A) Cell model of proximal tubule cells with transporters. The scheme shows the AQP2 transporter localized in the apical membrane and the Na+K+ATPase present in the basolateral membrane. (B-J) Cellular arrangement of ARPCs grown into the microfluidic device after 6 hs of FSS at 0.2 dyn/cm2. Two different chips were stained in (B-E) and in (G-J), respectively. Immunofluorescence images of stained DNA (DAPI, blue) (B, G), actin (TRITC-phalloidin, red) (C, H), AQP2 (FITC-anti goat, green) (D) and Na+K+ATPase pump (I). X-Z section confocal images for AQP2 (apical marker protein) (E) and Na+K+ATPase pump (basolateral marker protein) (J) following FSS in the chip. Scale bars  =  100 µm. The section along the longitudinal axis of the microchannel is indicated by the white line in D and I, respectively. X-Z section confocal images were also collected for AQP2 (F) and Na+K+ATPase pump (K) in statically cultured ARPCs. Scale bars  =  15 µm.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4048272&req=5

pone-0100141-g002: Polarization of ARPCs following FSS in the chip.(A) Cell model of proximal tubule cells with transporters. The scheme shows the AQP2 transporter localized in the apical membrane and the Na+K+ATPase present in the basolateral membrane. (B-J) Cellular arrangement of ARPCs grown into the microfluidic device after 6 hs of FSS at 0.2 dyn/cm2. Two different chips were stained in (B-E) and in (G-J), respectively. Immunofluorescence images of stained DNA (DAPI, blue) (B, G), actin (TRITC-phalloidin, red) (C, H), AQP2 (FITC-anti goat, green) (D) and Na+K+ATPase pump (I). X-Z section confocal images for AQP2 (apical marker protein) (E) and Na+K+ATPase pump (basolateral marker protein) (J) following FSS in the chip. Scale bars  =  100 µm. The section along the longitudinal axis of the microchannel is indicated by the white line in D and I, respectively. X-Z section confocal images were also collected for AQP2 (F) and Na+K+ATPase pump (K) in statically cultured ARPCs. Scale bars  =  15 µm.
Mentions: The figure legends for Figure 2 and 5 are incorrect. There are incorrect values presented for the scale bars in the legends. Please see these figures and their corrected legends below.

View Article: PubMed Central

No MeSH data available.


Related in: MedlinePlus