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Virus versus host plant microRNAs: who determines the outcome of the interaction?

Maghuly F, Ramkat RC, Laimer M - PLoS ONE (2014)

Bottom Line: Plant miRs/miRs* from conserved and highly expressed families were identified, which were shown to have potential targets in the genome of both begomoviruses, representing potential plant miRNAs mediating antiviral defense.This is the first assessment of predicted viral miRs/miRs* of ACMV and EACMV-UG and host plant miRNAs, providing a reference point for miRNA identification in pathogens and their hosts.These findings will improve the understanding of host- pathogen interaction pathways and the function of viral miRNAs in Euphorbiaceous crop plants.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Unit (PBU), Department Biotechnology, University of Natural Resources and Life Sciences, BOKU-VIBT, Vienna, Austria.

ABSTRACT
Considering the importance of microRNAs (miRNAs) in the regulation of essential processes in plant pathogen interactions, it is not surprising that, while plant miRNA sequences counteract viral attack via antiviral RNA silencing, viruses in turn have developed antihost defense mechanisms blocking these RNA silencing pathways and establish a counter-defense. In the current study, computational and stem-loop Reverse Transcription - Polymerase Chain Reaction (RT-PCR) approaches were employed to a) predict and validate virus encoded mature miRNAs (miRs) in 39 DNA-A sequences of the bipartite genomes of African cassava mosaic virus (ACMV) and East African cassava mosaic virus-Uganda (EACMV-UG) isolates, b) determine whether virus encoded miRs/miRs* generated from the 5'/3' harpin arms have the capacity to bind to genomic sequences of the host plants Jatropha or cassava and c) investigate whether plant encoded miR/miR* sequences have the potential to bind to the viral genomes. Different viral pre-miRNA hairpin sequences and viral miR/miR* length variants occurring as isomiRs were predicted in both viruses. These miRNAs were located in three Open Reading Frames (ORFs) and in the Intergenic Region (IR). Moreover, various target genes for miRNAs from both viruses were predicted and annotated in the host plant genomes indicating that they are involved in biotic response, metabolic pathways and transcription factors. Plant miRs/miRs* from conserved and highly expressed families were identified, which were shown to have potential targets in the genome of both begomoviruses, representing potential plant miRNAs mediating antiviral defense. This is the first assessment of predicted viral miRs/miRs* of ACMV and EACMV-UG and host plant miRNAs, providing a reference point for miRNA identification in pathogens and their hosts. These findings will improve the understanding of host- pathogen interaction pathways and the function of viral miRNAs in Euphorbiaceous crop plants.

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Related in: MedlinePlus

Outline of the strategy to identify miRNAs in viral DNA-A of ACMV and EACMV-UG and their potential targets in the host plants Jatropha and cassava.Computational approaches were used to scan and filter the DNA-A genomes of 11 ACMV and 28 EACMV-UG isolates to identify novel virus miRNAs and their targets in Jatropha and cassava.
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pone-0098263-g002: Outline of the strategy to identify miRNAs in viral DNA-A of ACMV and EACMV-UG and their potential targets in the host plants Jatropha and cassava.Computational approaches were used to scan and filter the DNA-A genomes of 11 ACMV and 28 EACMV-UG isolates to identify novel virus miRNAs and their targets in Jatropha and cassava.

Mentions: Computational approaches were used for the first time to scan and filter the DNA-A genomes of 11 ACMV and 28 EACMV-UG isolates to identify novel pre-miRNA hairpins encoding miRNAs, which could target the Jatropha and cassava genome (Table 1). Figure 2 gives an overview of the strategies adopted for the search and prediction of novel virus (ACMV and EACMV-UG) miRNAs matching targets in their host plants Jatropha and cassava. As a result, a total of 14 different predicted pre-miRNA hairpin sequences (9 from ACMV and 5 from EACMV-UG) were obtained (Table 2).


Virus versus host plant microRNAs: who determines the outcome of the interaction?

Maghuly F, Ramkat RC, Laimer M - PLoS ONE (2014)

Outline of the strategy to identify miRNAs in viral DNA-A of ACMV and EACMV-UG and their potential targets in the host plants Jatropha and cassava.Computational approaches were used to scan and filter the DNA-A genomes of 11 ACMV and 28 EACMV-UG isolates to identify novel virus miRNAs and their targets in Jatropha and cassava.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4045720&req=5

pone-0098263-g002: Outline of the strategy to identify miRNAs in viral DNA-A of ACMV and EACMV-UG and their potential targets in the host plants Jatropha and cassava.Computational approaches were used to scan and filter the DNA-A genomes of 11 ACMV and 28 EACMV-UG isolates to identify novel virus miRNAs and their targets in Jatropha and cassava.
Mentions: Computational approaches were used for the first time to scan and filter the DNA-A genomes of 11 ACMV and 28 EACMV-UG isolates to identify novel pre-miRNA hairpins encoding miRNAs, which could target the Jatropha and cassava genome (Table 1). Figure 2 gives an overview of the strategies adopted for the search and prediction of novel virus (ACMV and EACMV-UG) miRNAs matching targets in their host plants Jatropha and cassava. As a result, a total of 14 different predicted pre-miRNA hairpin sequences (9 from ACMV and 5 from EACMV-UG) were obtained (Table 2).

Bottom Line: Plant miRs/miRs* from conserved and highly expressed families were identified, which were shown to have potential targets in the genome of both begomoviruses, representing potential plant miRNAs mediating antiviral defense.This is the first assessment of predicted viral miRs/miRs* of ACMV and EACMV-UG and host plant miRNAs, providing a reference point for miRNA identification in pathogens and their hosts.These findings will improve the understanding of host- pathogen interaction pathways and the function of viral miRNAs in Euphorbiaceous crop plants.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Unit (PBU), Department Biotechnology, University of Natural Resources and Life Sciences, BOKU-VIBT, Vienna, Austria.

ABSTRACT
Considering the importance of microRNAs (miRNAs) in the regulation of essential processes in plant pathogen interactions, it is not surprising that, while plant miRNA sequences counteract viral attack via antiviral RNA silencing, viruses in turn have developed antihost defense mechanisms blocking these RNA silencing pathways and establish a counter-defense. In the current study, computational and stem-loop Reverse Transcription - Polymerase Chain Reaction (RT-PCR) approaches were employed to a) predict and validate virus encoded mature miRNAs (miRs) in 39 DNA-A sequences of the bipartite genomes of African cassava mosaic virus (ACMV) and East African cassava mosaic virus-Uganda (EACMV-UG) isolates, b) determine whether virus encoded miRs/miRs* generated from the 5'/3' harpin arms have the capacity to bind to genomic sequences of the host plants Jatropha or cassava and c) investigate whether plant encoded miR/miR* sequences have the potential to bind to the viral genomes. Different viral pre-miRNA hairpin sequences and viral miR/miR* length variants occurring as isomiRs were predicted in both viruses. These miRNAs were located in three Open Reading Frames (ORFs) and in the Intergenic Region (IR). Moreover, various target genes for miRNAs from both viruses were predicted and annotated in the host plant genomes indicating that they are involved in biotic response, metabolic pathways and transcription factors. Plant miRs/miRs* from conserved and highly expressed families were identified, which were shown to have potential targets in the genome of both begomoviruses, representing potential plant miRNAs mediating antiviral defense. This is the first assessment of predicted viral miRs/miRs* of ACMV and EACMV-UG and host plant miRNAs, providing a reference point for miRNA identification in pathogens and their hosts. These findings will improve the understanding of host- pathogen interaction pathways and the function of viral miRNAs in Euphorbiaceous crop plants.

Show MeSH
Related in: MedlinePlus