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Impact of transgenic wheat with wheat yellow mosaic virus resistance on microbial community diversity and enzyme activity in rhizosphere soil.

Wu J, Yu M, Xu J, Du J, Ji F, Dong F, Li X, Shi J - PLoS ONE (2014)

Bottom Line: The results showed that there was little difference in bacterial and fungal community diversity in rhizosphere soil between N12-1 and its recipient Y158 by comparing Shannon's, Simpson's diversity index and evenness (except at one or two growth stages).Regarding enzyme activity, only one significant difference was found during the maturing stage at Xinxiang in 2011 for dehydrogenase.The results of this study suggested that virus-resistant transgenic wheat had no adverse impact on microbial community diversity and enzyme activity in rhizosphere soil during 2 continuous years at two different experimental locations.

View Article: PubMed Central - PubMed

Affiliation: Institute of Food Safety and Detection, Jiangsu Academy of Agricultural Sciences, Nanjing, China; Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing, China; Jiangsu Center for GMO evaluation and detection, Nanjing, China.

ABSTRACT
The transgenic wheat line N12-1 containing the WYMV-Nib8 gene was obtained previously through particle bombardment, and it can effectively control the wheat yellow mosaic virus (WYMV) disease transmitted by Polymyxa graminis at turngreen stage. Due to insertion of an exogenous gene, the transcriptome of wheat may be altered and affect root exudates. Thus, it is important to investigate the potential environmental risk of transgenic wheat before commercial release because of potential undesirable ecological side effects. Our 2-year study at two different experimental locations was performed to analyze the impact of transgenic wheat N12-1 on bacterial and fungal community diversity in rhizosphere soil using polymerase chain reaction-denaturing gel gradient electrophoresis (PCR-DGGE) at four growth stages (seeding stage, turngreen stage, grain-filling stage, and maturing stage). We also explored the activities of urease, sucrase and dehydrogenase in rhizosphere soil. The results showed that there was little difference in bacterial and fungal community diversity in rhizosphere soil between N12-1 and its recipient Y158 by comparing Shannon's, Simpson's diversity index and evenness (except at one or two growth stages). Regarding enzyme activity, only one significant difference was found during the maturing stage at Xinxiang in 2011 for dehydrogenase. Significant growth stage variation was observed during 2 years at two experimental locations for both soil microbial community diversity and enzyme activity. Analysis of bands from the gel for fungal community diversity showed that the majority of fungi were uncultured. The results of this study suggested that virus-resistant transgenic wheat had no adverse impact on microbial community diversity and enzyme activity in rhizosphere soil during 2 continuous years at two different experimental locations. This study provides a theoretical basis for environmental impact monitoring of transgenic wheat when the introduced gene is derived from a virus.

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Principal component analysis of fungi communities diversity in rhizosphere soil.A: Luhe in 2011; B: Luhe in 2012; C: Xinxiang in 2011; D: Xinxiang in 2012. Square: N12-1; Round: Y158. Gray: seeding stage; Green: turngreen stage; Red: grainfilling stage; Yellow: maturing stage. Band position and presence (presence/absence) were used to carry out PCA analyses.
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pone-0098394-g005: Principal component analysis of fungi communities diversity in rhizosphere soil.A: Luhe in 2011; B: Luhe in 2012; C: Xinxiang in 2011; D: Xinxiang in 2012. Square: N12-1; Round: Y158. Gray: seeding stage; Green: turngreen stage; Red: grainfilling stage; Yellow: maturing stage. Band position and presence (presence/absence) were used to carry out PCA analyses.

Mentions: For experiments at Luhe, the contribution rates of the two principal components were 36.93% and 19.45% in 2011 (Fig. 5A) and 27.22% and 16.31% in 2012 (Fig. 5B), respectively. Different sampling times showed a distinct separation along the principal components axes, whereas different replications of experiment materials formed a cluster at the same sampling time. In 2011, the first principal component axis clearly separated the four growth stages (Fig. 5A), but separated SS and TS from GS and MS in 2012 (Fig. 5B). The second principal component axis clearly distinguished the TS stage in 2011 (Fig. 5A), and the SS and TS stage in 2012 (Fig. 5B).


Impact of transgenic wheat with wheat yellow mosaic virus resistance on microbial community diversity and enzyme activity in rhizosphere soil.

Wu J, Yu M, Xu J, Du J, Ji F, Dong F, Li X, Shi J - PLoS ONE (2014)

Principal component analysis of fungi communities diversity in rhizosphere soil.A: Luhe in 2011; B: Luhe in 2012; C: Xinxiang in 2011; D: Xinxiang in 2012. Square: N12-1; Round: Y158. Gray: seeding stage; Green: turngreen stage; Red: grainfilling stage; Yellow: maturing stage. Band position and presence (presence/absence) were used to carry out PCA analyses.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4045665&req=5

pone-0098394-g005: Principal component analysis of fungi communities diversity in rhizosphere soil.A: Luhe in 2011; B: Luhe in 2012; C: Xinxiang in 2011; D: Xinxiang in 2012. Square: N12-1; Round: Y158. Gray: seeding stage; Green: turngreen stage; Red: grainfilling stage; Yellow: maturing stage. Band position and presence (presence/absence) were used to carry out PCA analyses.
Mentions: For experiments at Luhe, the contribution rates of the two principal components were 36.93% and 19.45% in 2011 (Fig. 5A) and 27.22% and 16.31% in 2012 (Fig. 5B), respectively. Different sampling times showed a distinct separation along the principal components axes, whereas different replications of experiment materials formed a cluster at the same sampling time. In 2011, the first principal component axis clearly separated the four growth stages (Fig. 5A), but separated SS and TS from GS and MS in 2012 (Fig. 5B). The second principal component axis clearly distinguished the TS stage in 2011 (Fig. 5A), and the SS and TS stage in 2012 (Fig. 5B).

Bottom Line: The results showed that there was little difference in bacterial and fungal community diversity in rhizosphere soil between N12-1 and its recipient Y158 by comparing Shannon's, Simpson's diversity index and evenness (except at one or two growth stages).Regarding enzyme activity, only one significant difference was found during the maturing stage at Xinxiang in 2011 for dehydrogenase.The results of this study suggested that virus-resistant transgenic wheat had no adverse impact on microbial community diversity and enzyme activity in rhizosphere soil during 2 continuous years at two different experimental locations.

View Article: PubMed Central - PubMed

Affiliation: Institute of Food Safety and Detection, Jiangsu Academy of Agricultural Sciences, Nanjing, China; Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing, China; Jiangsu Center for GMO evaluation and detection, Nanjing, China.

ABSTRACT
The transgenic wheat line N12-1 containing the WYMV-Nib8 gene was obtained previously through particle bombardment, and it can effectively control the wheat yellow mosaic virus (WYMV) disease transmitted by Polymyxa graminis at turngreen stage. Due to insertion of an exogenous gene, the transcriptome of wheat may be altered and affect root exudates. Thus, it is important to investigate the potential environmental risk of transgenic wheat before commercial release because of potential undesirable ecological side effects. Our 2-year study at two different experimental locations was performed to analyze the impact of transgenic wheat N12-1 on bacterial and fungal community diversity in rhizosphere soil using polymerase chain reaction-denaturing gel gradient electrophoresis (PCR-DGGE) at four growth stages (seeding stage, turngreen stage, grain-filling stage, and maturing stage). We also explored the activities of urease, sucrase and dehydrogenase in rhizosphere soil. The results showed that there was little difference in bacterial and fungal community diversity in rhizosphere soil between N12-1 and its recipient Y158 by comparing Shannon's, Simpson's diversity index and evenness (except at one or two growth stages). Regarding enzyme activity, only one significant difference was found during the maturing stage at Xinxiang in 2011 for dehydrogenase. Significant growth stage variation was observed during 2 years at two experimental locations for both soil microbial community diversity and enzyme activity. Analysis of bands from the gel for fungal community diversity showed that the majority of fungi were uncultured. The results of this study suggested that virus-resistant transgenic wheat had no adverse impact on microbial community diversity and enzyme activity in rhizosphere soil during 2 continuous years at two different experimental locations. This study provides a theoretical basis for environmental impact monitoring of transgenic wheat when the introduced gene is derived from a virus.

Show MeSH
Related in: MedlinePlus