Limits...
In vivo characterization of abdominal aortic aneurysms using an elastin specific molecular MR probe

View Article: PubMed Central - HTML

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Rupture of abdominal aortic aneurysms (AAAs) is the third most common cause of death in cardiovascular diseases... Elastin is the key protein for maintaining stability of the aortic wall... The aim of this study was to evaluate a novel small-molecular-weight elastin-specific MR probe for the in-vivo assessment of aortic wall integrity in AAAs... An elastin-specific MR probe (Lantheus Medical Imaging, USA) was administered 1, 2, 3 and 4 weeks following Ang-II infusion... Imaging parameters of 3D IR-MRI: spatial resolution = 0.1 × 0.1 mm, 0.5 mm slice-thickness, TR/TE = 28/8.2 ms... Additionally 3D-T1 mapping was performed... After one week of Ang-II infusion, the elastin-specific probe enabled the clear in-vivo visualization of aortic rupture sites prior to the dilation of the lumen (Figure 1A)... After 2 and 3 weeks of Ang-II infusion, a significant (p < 0.05) increase in luminal diameter was observed resulting from the rupture of elastic laminae (Figure 1B)... The gadolinium concentration in AAAs increased as expression of elastin progressed... A significant correlation of CNR (p < 0.05) and R1 (p < 0.05) with ex-vivo gadolinium concentrations (ICP-MS) was found (Figure 2)... After Gd-DTPA administration, no significant increase (p > 0.05) in R1 could be measured compared to pre-contrast scans... The elastin-specific molecular MR probe allows for the visualization and quantification of changes in elastin content at different stages of AAAs... This clinically translatable probe offers potential for the non-invasive detection of rupture sites prior to aortic dilation and subsequent monitoring of compensatory repair processes... This could enable a more accurate risk stratification and help guiding treatment decisions.

No MeSH data available.


Related in: MedlinePlus

A: Aortic angiogram of an ApoE-/- mouse 1 week after of Ang-II infusion without luminal dilatation or irregularities (A1). On pre-contrast (A2) and Gd-DTPA (A3) enhanced images only minor wall enhancement was observed. On ESMA-MRI the rupture of elastic laminae was clearly visualized in vivo (A4, A5), which was confirmed in corresponding histological sections (A6, A7). B: Aortic angiogram (B1) and fusion with ESMA scan (B2) 3 weeks after Ang-II. A significant increase in luminal diameter was observed resulting from the rupture of elastic laminae (B7, B8). We observed a strong increase in elastin formation at the site of the hematoma (B8, red arrows). Newly formed elastic fibers bridged the area in-between dissected elastic laminae (B8). This repair process was visualized by a strong enhancement on ESMA-MRI (B5, B6). On pre-contrast (B3) and Gd-DTPA (B4) images only minor enhancement was observed. The undisrupted wall showed only a moderate enhancement (*). aA: abdominal aorta, rRA: right renal artery.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4044089&req=5

Figure 1: A: Aortic angiogram of an ApoE-/- mouse 1 week after of Ang-II infusion without luminal dilatation or irregularities (A1). On pre-contrast (A2) and Gd-DTPA (A3) enhanced images only minor wall enhancement was observed. On ESMA-MRI the rupture of elastic laminae was clearly visualized in vivo (A4, A5), which was confirmed in corresponding histological sections (A6, A7). B: Aortic angiogram (B1) and fusion with ESMA scan (B2) 3 weeks after Ang-II. A significant increase in luminal diameter was observed resulting from the rupture of elastic laminae (B7, B8). We observed a strong increase in elastin formation at the site of the hematoma (B8, red arrows). Newly formed elastic fibers bridged the area in-between dissected elastic laminae (B8). This repair process was visualized by a strong enhancement on ESMA-MRI (B5, B6). On pre-contrast (B3) and Gd-DTPA (B4) images only minor enhancement was observed. The undisrupted wall showed only a moderate enhancement (*). aA: abdominal aorta, rRA: right renal artery.

Mentions: Imaging was performed with a spatial resolution of 100 μm on a clinical MRI system using clinical imaging protocols. After one week of Ang-II infusion, the elastin-specific probe enabled the clear in-vivo visualization of aortic rupture sites prior to the dilation of the lumen (Figure 1A). After 2 and 3 weeks of Ang-II infusion, a significant (p < 0.05) increase in luminal diameter was observed resulting from the rupture of elastic laminae (Figure 1B). We observed a strong increase in elastin formation at the site of the hematoma. Newly formed elastic fibers bridged the area in-between dissected elastic laminae. This repair process was clearly visualized and quantified by the elastin-specific probe. Using electron microscopy, co-localization of the probe with elastic fibers was found. The gadolinium concentration in AAAs increased as expression of elastin progressed. A significant correlation of CNR (p < 0.05) and R1 (p < 0.05) with ex-vivo gadolinium concentrations (ICP-MS) was found (Figure 2). After Gd-DTPA administration, no significant increase (p > 0.05) in R1 could be measured compared to pre-contrast scans.


In vivo characterization of abdominal aortic aneurysms using an elastin specific molecular MR probe
A: Aortic angiogram of an ApoE-/- mouse 1 week after of Ang-II infusion without luminal dilatation or irregularities (A1). On pre-contrast (A2) and Gd-DTPA (A3) enhanced images only minor wall enhancement was observed. On ESMA-MRI the rupture of elastic laminae was clearly visualized in vivo (A4, A5), which was confirmed in corresponding histological sections (A6, A7). B: Aortic angiogram (B1) and fusion with ESMA scan (B2) 3 weeks after Ang-II. A significant increase in luminal diameter was observed resulting from the rupture of elastic laminae (B7, B8). We observed a strong increase in elastin formation at the site of the hematoma (B8, red arrows). Newly formed elastic fibers bridged the area in-between dissected elastic laminae (B8). This repair process was visualized by a strong enhancement on ESMA-MRI (B5, B6). On pre-contrast (B3) and Gd-DTPA (B4) images only minor enhancement was observed. The undisrupted wall showed only a moderate enhancement (*). aA: abdominal aorta, rRA: right renal artery.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4044089&req=5

Figure 1: A: Aortic angiogram of an ApoE-/- mouse 1 week after of Ang-II infusion without luminal dilatation or irregularities (A1). On pre-contrast (A2) and Gd-DTPA (A3) enhanced images only minor wall enhancement was observed. On ESMA-MRI the rupture of elastic laminae was clearly visualized in vivo (A4, A5), which was confirmed in corresponding histological sections (A6, A7). B: Aortic angiogram (B1) and fusion with ESMA scan (B2) 3 weeks after Ang-II. A significant increase in luminal diameter was observed resulting from the rupture of elastic laminae (B7, B8). We observed a strong increase in elastin formation at the site of the hematoma (B8, red arrows). Newly formed elastic fibers bridged the area in-between dissected elastic laminae (B8). This repair process was visualized by a strong enhancement on ESMA-MRI (B5, B6). On pre-contrast (B3) and Gd-DTPA (B4) images only minor enhancement was observed. The undisrupted wall showed only a moderate enhancement (*). aA: abdominal aorta, rRA: right renal artery.
Mentions: Imaging was performed with a spatial resolution of 100 μm on a clinical MRI system using clinical imaging protocols. After one week of Ang-II infusion, the elastin-specific probe enabled the clear in-vivo visualization of aortic rupture sites prior to the dilation of the lumen (Figure 1A). After 2 and 3 weeks of Ang-II infusion, a significant (p < 0.05) increase in luminal diameter was observed resulting from the rupture of elastic laminae (Figure 1B). We observed a strong increase in elastin formation at the site of the hematoma. Newly formed elastic fibers bridged the area in-between dissected elastic laminae. This repair process was clearly visualized and quantified by the elastin-specific probe. Using electron microscopy, co-localization of the probe with elastic fibers was found. The gadolinium concentration in AAAs increased as expression of elastin progressed. A significant correlation of CNR (p < 0.05) and R1 (p < 0.05) with ex-vivo gadolinium concentrations (ICP-MS) was found (Figure 2). After Gd-DTPA administration, no significant increase (p > 0.05) in R1 could be measured compared to pre-contrast scans.

View Article: PubMed Central - HTML

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Rupture of abdominal aortic aneurysms (AAAs) is the third most common cause of death in cardiovascular diseases... Elastin is the key protein for maintaining stability of the aortic wall... The aim of this study was to evaluate a novel small-molecular-weight elastin-specific MR probe for the in-vivo assessment of aortic wall integrity in AAAs... An elastin-specific MR probe (Lantheus Medical Imaging, USA) was administered 1, 2, 3 and 4 weeks following Ang-II infusion... Imaging parameters of 3D IR-MRI: spatial resolution = 0.1 × 0.1 mm, 0.5 mm slice-thickness, TR/TE = 28/8.2 ms... Additionally 3D-T1 mapping was performed... After one week of Ang-II infusion, the elastin-specific probe enabled the clear in-vivo visualization of aortic rupture sites prior to the dilation of the lumen (Figure 1A)... After 2 and 3 weeks of Ang-II infusion, a significant (p < 0.05) increase in luminal diameter was observed resulting from the rupture of elastic laminae (Figure 1B)... The gadolinium concentration in AAAs increased as expression of elastin progressed... A significant correlation of CNR (p < 0.05) and R1 (p < 0.05) with ex-vivo gadolinium concentrations (ICP-MS) was found (Figure 2)... After Gd-DTPA administration, no significant increase (p > 0.05) in R1 could be measured compared to pre-contrast scans... The elastin-specific molecular MR probe allows for the visualization and quantification of changes in elastin content at different stages of AAAs... This clinically translatable probe offers potential for the non-invasive detection of rupture sites prior to aortic dilation and subsequent monitoring of compensatory repair processes... This could enable a more accurate risk stratification and help guiding treatment decisions.

No MeSH data available.


Related in: MedlinePlus