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Effect of bile pigments on the compromised gut barrier function in a rat model of bile duct ligation.

Zhou K, Jiang M, Liu Y, Qu Y, Shi G, Yang X, Qin X, Wang X - PLoS ONE (2014)

Bottom Line: Trypsin and chymotrypsin of the gut were also measured to determine how these digestive proteases may relate to the observed effects of bile pigments.Free bilirubin but not bilirubin ditaurate or biliverdin showed significant inhibitions on trypsin and chymotrypsin as well as alleviated changes of histological and biochemical parameters related to gut barrier disruption.Bile may protect the gut from damage through inhibiting digestive proteases like trypsin and chymotrypsin by free bilirubin.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Heilongjiang Provincial Science and Technology Innovation Team in Higher Education Institutes for Infection and Immunity, Harbin Medical University, Harbin, China.

ABSTRACT

Background: Studies have shown that the absence of bile in the gut lumen, either by bile duct ligation or bile diversion, induces mucosal injury. However, the mechanism remains elusive. In this study, the role of bile pigments in gut barrier function was investigated in a rat model of bile duct ligation.

Methods: Male Sprague Dawley (SD) rats were used in this study. After ligation of bile duct, the animals were administrated with free bilirubin, bilirubin ditaurate, or biliverdin by intragastric gavage. 1, 2, or 3 days later, the animals were sacrificed and the damage of mucosa was assessed by histological staining as well as biochemical parameters such as changes of diamine oxidase (DAO) and D-lactate (D-Lac) in the blood. Trypsin and chymotrypsin of the gut were also measured to determine how these digestive proteases may relate to the observed effects of bile pigments.

Results: Bile duct ligation (BDL) caused significant increases in gut trypsin and chymotrypsin along with damage of the mucosa as demonstrated by the histological findings under microscope, the reduced expression of tight junction molecules like occludin, and significant changes in DAO and D-lac in the blood. Free bilirubin but not bilirubin ditaurate or biliverdin showed significant inhibitions on trypsin and chymotrypsin as well as alleviated changes of histological and biochemical parameters related to gut barrier disruption.

Conclusion: Bile may protect the gut from damage through inhibiting digestive proteases like trypsin and chymotrypsin by free bilirubin.

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Related in: MedlinePlus

Effect of 0.1 mM bile pigments on trypsin, chymotrypsin levels.After ligation of bile duct, the animals were administrated with free bilirubin, bilirubin ditaurate, or biliverdin by intragastric gavage. The amounts of trypsin (A) and chymotrypsin (B) in middle small intestine were assayed by ELISA kit after 48 h. The data are expressed as mean ± SD from 8 independent animals, *p<0.05 **p<0.01 vs. respective control vs. respective BDL 48 h group.
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pone-0098905-g004: Effect of 0.1 mM bile pigments on trypsin, chymotrypsin levels.After ligation of bile duct, the animals were administrated with free bilirubin, bilirubin ditaurate, or biliverdin by intragastric gavage. The amounts of trypsin (A) and chymotrypsin (B) in middle small intestine were assayed by ELISA kit after 48 h. The data are expressed as mean ± SD from 8 independent animals, *p<0.05 **p<0.01 vs. respective control vs. respective BDL 48 h group.

Mentions: As shown in Figure 4, 48 h after bilirubin ditaurate administration no changes in trypsin or chymotrypsin levels were observed in intestinal segment as compared to BDL 48 h group. The concentrations of trypsin, chymotrypsin in UCB administration group reduced by 17.8% (p<0.05) and 18.9% (p<0.01), respectively. On the contrary, these two digestive proteases levels were dramatically increased (increase of 10.4% and 5.6%, respectively) in biliverdin administration group. We can see that trypsin and chymotrypsin were inhibited by 0.1 mM UCB, but no significant changes were observed in bilirubin ditaurate administration group. In contrast, 0.1 mM biliverdin showed some stimulation on trypsin and chymotrypsin.


Effect of bile pigments on the compromised gut barrier function in a rat model of bile duct ligation.

Zhou K, Jiang M, Liu Y, Qu Y, Shi G, Yang X, Qin X, Wang X - PLoS ONE (2014)

Effect of 0.1 mM bile pigments on trypsin, chymotrypsin levels.After ligation of bile duct, the animals were administrated with free bilirubin, bilirubin ditaurate, or biliverdin by intragastric gavage. The amounts of trypsin (A) and chymotrypsin (B) in middle small intestine were assayed by ELISA kit after 48 h. The data are expressed as mean ± SD from 8 independent animals, *p<0.05 **p<0.01 vs. respective control vs. respective BDL 48 h group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4044053&req=5

pone-0098905-g004: Effect of 0.1 mM bile pigments on trypsin, chymotrypsin levels.After ligation of bile duct, the animals were administrated with free bilirubin, bilirubin ditaurate, or biliverdin by intragastric gavage. The amounts of trypsin (A) and chymotrypsin (B) in middle small intestine were assayed by ELISA kit after 48 h. The data are expressed as mean ± SD from 8 independent animals, *p<0.05 **p<0.01 vs. respective control vs. respective BDL 48 h group.
Mentions: As shown in Figure 4, 48 h after bilirubin ditaurate administration no changes in trypsin or chymotrypsin levels were observed in intestinal segment as compared to BDL 48 h group. The concentrations of trypsin, chymotrypsin in UCB administration group reduced by 17.8% (p<0.05) and 18.9% (p<0.01), respectively. On the contrary, these two digestive proteases levels were dramatically increased (increase of 10.4% and 5.6%, respectively) in biliverdin administration group. We can see that trypsin and chymotrypsin were inhibited by 0.1 mM UCB, but no significant changes were observed in bilirubin ditaurate administration group. In contrast, 0.1 mM biliverdin showed some stimulation on trypsin and chymotrypsin.

Bottom Line: Trypsin and chymotrypsin of the gut were also measured to determine how these digestive proteases may relate to the observed effects of bile pigments.Free bilirubin but not bilirubin ditaurate or biliverdin showed significant inhibitions on trypsin and chymotrypsin as well as alleviated changes of histological and biochemical parameters related to gut barrier disruption.Bile may protect the gut from damage through inhibiting digestive proteases like trypsin and chymotrypsin by free bilirubin.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Heilongjiang Provincial Science and Technology Innovation Team in Higher Education Institutes for Infection and Immunity, Harbin Medical University, Harbin, China.

ABSTRACT

Background: Studies have shown that the absence of bile in the gut lumen, either by bile duct ligation or bile diversion, induces mucosal injury. However, the mechanism remains elusive. In this study, the role of bile pigments in gut barrier function was investigated in a rat model of bile duct ligation.

Methods: Male Sprague Dawley (SD) rats were used in this study. After ligation of bile duct, the animals were administrated with free bilirubin, bilirubin ditaurate, or biliverdin by intragastric gavage. 1, 2, or 3 days later, the animals were sacrificed and the damage of mucosa was assessed by histological staining as well as biochemical parameters such as changes of diamine oxidase (DAO) and D-lactate (D-Lac) in the blood. Trypsin and chymotrypsin of the gut were also measured to determine how these digestive proteases may relate to the observed effects of bile pigments.

Results: Bile duct ligation (BDL) caused significant increases in gut trypsin and chymotrypsin along with damage of the mucosa as demonstrated by the histological findings under microscope, the reduced expression of tight junction molecules like occludin, and significant changes in DAO and D-lac in the blood. Free bilirubin but not bilirubin ditaurate or biliverdin showed significant inhibitions on trypsin and chymotrypsin as well as alleviated changes of histological and biochemical parameters related to gut barrier disruption.

Conclusion: Bile may protect the gut from damage through inhibiting digestive proteases like trypsin and chymotrypsin by free bilirubin.

Show MeSH
Related in: MedlinePlus