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The role of voltage-gated calcium channels in neurotransmitter phenotype specification: Coexpression and functional analysis in Xenopus laevis.

Lewis BB, Miller LE, Herbst WA, Saha MS - J. Comp. Neurol. (2014)

Bottom Line: Calcium activity was also analyzed on a single-cell level, and spike frequency was correlated with the expression of VGCC α1 subunits in cell culture.Cells expressing Cav 2.1 and Cav 2.2 displayed increased calcium spiking compared with cells not expressing this marker.The VGCC antagonist diltiazem and agonist (-)BayK 8644 were used to manipulate calcium activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, College of William and Mary, Williamsburg, Virginia, 23185.

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Expression of VGCCs in cultured cells. Expression of the five VGCC α1 subunits detected with FISH in neural cell cultures during early development. Cav1.2 and Cav1.3 are detected at neural plate and neural tube stages, respectively. Cav2.1, Cav2.2, and Cav3.2 are detected throughout the developmental period studied. Cav2.1 and Cav2.2 are expressed at highest levels at the neural fold stage, and Cav3.2 expression is highest at the neural tube stage. At the neural plate stage, n = 280 cells (Cav1.2), 339 cells (Cav2.1), 358 cells (Cav2.2), and 578 cells (Cav3.2). At the neural fold stage, n = 376 cells (Cav2.1), 305 cells (Cav2.2), and 403 cells (Cav3.2). At the neural tube stage, n = 1,843 cells (Cav1.3), 1,227 cells (Cav2.1), 1,197 cells (Cav2.2), and 631 cells (Cav3.2).
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fig05: Expression of VGCCs in cultured cells. Expression of the five VGCC α1 subunits detected with FISH in neural cell cultures during early development. Cav1.2 and Cav1.3 are detected at neural plate and neural tube stages, respectively. Cav2.1, Cav2.2, and Cav3.2 are detected throughout the developmental period studied. Cav2.1 and Cav2.2 are expressed at highest levels at the neural fold stage, and Cav3.2 expression is highest at the neural tube stage. At the neural plate stage, n = 280 cells (Cav1.2), 339 cells (Cav2.1), 358 cells (Cav2.2), and 578 cells (Cav3.2). At the neural fold stage, n = 376 cells (Cav2.1), 305 cells (Cav2.2), and 403 cells (Cav3.2). At the neural tube stage, n = 1,843 cells (Cav1.3), 1,227 cells (Cav2.1), 1,197 cells (Cav2.2), and 631 cells (Cav3.2).

Mentions: After determining the coexpression of VGCC α1 subunits with neurotransmitter markers in whole-mount embryos, VGCC expression was correlated with calcium activity in primary cell culture. Expression was assessed at the neural plate (st. 14), neural fold (st. 18), and neural tube (st. 22) stages by using FISH. Cav1.2, Cav2.1, Cav2.2, and Cav3.2 are the only channels detected in cultures dissected at the neural plate stage (Fig. 5). Whereas Cav2.1, Cav2.2, and Cav3.2 are subsequently expressed at neural fold and neural tube stage dissections, Cav1.2 is not detected in cells cultured after the neural plate stage. Cav1.3 is detected in cell cultures dissected at the neural tube stage.


The role of voltage-gated calcium channels in neurotransmitter phenotype specification: Coexpression and functional analysis in Xenopus laevis.

Lewis BB, Miller LE, Herbst WA, Saha MS - J. Comp. Neurol. (2014)

Expression of VGCCs in cultured cells. Expression of the five VGCC α1 subunits detected with FISH in neural cell cultures during early development. Cav1.2 and Cav1.3 are detected at neural plate and neural tube stages, respectively. Cav2.1, Cav2.2, and Cav3.2 are detected throughout the developmental period studied. Cav2.1 and Cav2.2 are expressed at highest levels at the neural fold stage, and Cav3.2 expression is highest at the neural tube stage. At the neural plate stage, n = 280 cells (Cav1.2), 339 cells (Cav2.1), 358 cells (Cav2.2), and 578 cells (Cav3.2). At the neural fold stage, n = 376 cells (Cav2.1), 305 cells (Cav2.2), and 403 cells (Cav3.2). At the neural tube stage, n = 1,843 cells (Cav1.3), 1,227 cells (Cav2.1), 1,197 cells (Cav2.2), and 631 cells (Cav3.2).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4043876&req=5

fig05: Expression of VGCCs in cultured cells. Expression of the five VGCC α1 subunits detected with FISH in neural cell cultures during early development. Cav1.2 and Cav1.3 are detected at neural plate and neural tube stages, respectively. Cav2.1, Cav2.2, and Cav3.2 are detected throughout the developmental period studied. Cav2.1 and Cav2.2 are expressed at highest levels at the neural fold stage, and Cav3.2 expression is highest at the neural tube stage. At the neural plate stage, n = 280 cells (Cav1.2), 339 cells (Cav2.1), 358 cells (Cav2.2), and 578 cells (Cav3.2). At the neural fold stage, n = 376 cells (Cav2.1), 305 cells (Cav2.2), and 403 cells (Cav3.2). At the neural tube stage, n = 1,843 cells (Cav1.3), 1,227 cells (Cav2.1), 1,197 cells (Cav2.2), and 631 cells (Cav3.2).
Mentions: After determining the coexpression of VGCC α1 subunits with neurotransmitter markers in whole-mount embryos, VGCC expression was correlated with calcium activity in primary cell culture. Expression was assessed at the neural plate (st. 14), neural fold (st. 18), and neural tube (st. 22) stages by using FISH. Cav1.2, Cav2.1, Cav2.2, and Cav3.2 are the only channels detected in cultures dissected at the neural plate stage (Fig. 5). Whereas Cav2.1, Cav2.2, and Cav3.2 are subsequently expressed at neural fold and neural tube stage dissections, Cav1.2 is not detected in cells cultured after the neural plate stage. Cav1.3 is detected in cell cultures dissected at the neural tube stage.

Bottom Line: Calcium activity was also analyzed on a single-cell level, and spike frequency was correlated with the expression of VGCC α1 subunits in cell culture.Cells expressing Cav 2.1 and Cav 2.2 displayed increased calcium spiking compared with cells not expressing this marker.The VGCC antagonist diltiazem and agonist (-)BayK 8644 were used to manipulate calcium activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, College of William and Mary, Williamsburg, Virginia, 23185.

Show MeSH
Related in: MedlinePlus