The role of voltage-gated calcium channels in neurotransmitter phenotype specification: Coexpression and functional analysis in Xenopus laevis.
Bottom Line: Calcium activity was also analyzed on a single-cell level, and spike frequency was correlated with the expression of VGCC α1 subunits in cell culture.Cells expressing Cav 2.1 and Cav 2.2 displayed increased calcium spiking compared with cells not expressing this marker.The VGCC antagonist diltiazem and agonist (-)BayK 8644 were used to manipulate calcium activity.
Affiliation: Department of Biology, College of William and Mary, Williamsburg, Virginia, 23185.Show MeSH
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Mentions: After determining the coexpression of VGCC α1 subunits with neurotransmitter markers in whole-mount embryos, VGCC expression was correlated with calcium activity in primary cell culture. Expression was assessed at the neural plate (st. 14), neural fold (st. 18), and neural tube (st. 22) stages by using FISH. Cav1.2, Cav2.1, Cav2.2, and Cav3.2 are the only channels detected in cultures dissected at the neural plate stage (Fig. 5). Whereas Cav2.1, Cav2.2, and Cav3.2 are subsequently expressed at neural fold and neural tube stage dissections, Cav1.2 is not detected in cells cultured after the neural plate stage. Cav1.3 is detected in cell cultures dissected at the neural tube stage.
Affiliation: Department of Biology, College of William and Mary, Williamsburg, Virginia, 23185.