The role of voltage-gated calcium channels in neurotransmitter phenotype specification: Coexpression and functional analysis in Xenopus laevis.
Bottom Line: Calcium activity was also analyzed on a single-cell level, and spike frequency was correlated with the expression of VGCC α1 subunits in cell culture.Cells expressing Cav 2.1 and Cav 2.2 displayed increased calcium spiking compared with cells not expressing this marker.The VGCC antagonist diltiazem and agonist (-)BayK 8644 were used to manipulate calcium activity.
Affiliation: Department of Biology, College of William and Mary, Williamsburg, Virginia, 23185.Show MeSH
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Mentions: To determine whether VGCC α1 subunits are coexpressed with inhibitory neurotransmitter markers, multiplex FISH analysis was performed, probing for VGCCs and xVIAAT. To a lesser extent, VGCC α1 subunits are coexpressed with the inhibitory neural marker (Table3). Forebrain and midbrain coexpression is restricted to ventral regions and occurs most prominently with Cav1.2, Cav2.1, and Cav2.2 (Fig. 3A,B,K,L,P,Q). Hindbrain coexpression is located dorsally and occurs with Cav1.2, Cav1.3, and Cav2.2 (Fig. 3C,H,R). In the spinal cord, coexpression is found with Cav1.2, Cav1.3, Cav2.1, and Cav2.2 in the inhibitory commissural reciprocal interneurons (cINs) and ascending recurrent interneurons (aINs) (Fig. 3D,I,N,S). Retina coexpression occurs in the inner nuclear layer (INL) with Cav2.1 and Cav2.2 (Fig. 4D,E).
Affiliation: Department of Biology, College of William and Mary, Williamsburg, Virginia, 23185.