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Resolvin D1 and lipoxin A4 improve alveolarization and normalize septal wall thickness in a neonatal murine model of hyperoxia-induced lung injury.

Martin CR, Zaman MM, Gilkey C, Salguero MV, Hasturk H, Kantarci A, Van Dyke TE, Freedman SD - PLoS ONE (2014)

Bottom Line: To determine the effect of Resolvin D1 and/or Lipoxin A4 on hyperoxia-induced lung injury.Treatment with Lipoxin A4 also led to a reduction of CXCL2 (2.4 fold) while selectively increasing TGFβ2 (2.1 fold) and Smad3 (1.58 fold).These fatty acids or their metabolites may be novel therapies to prevent or treat lung injury in preterm infants.

View Article: PubMed Central - PubMed

Affiliation: Department of Neonatology, Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States of America; Division of Translational Research, Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States of America.

ABSTRACT

Background: The critical fatty acids Docosahexaenoic Acid (DHA) and Arachidonic Acid (AA) decline in preterm infants within the first postnatal week and are associated with neonatal morbidities, including bronchopulmonary dysplasia (BPD). DHA and AA are precursors to downstream metabolites that terminate the inflammatory response. We hypothesized that treatment with Resolvin D1 and/or Lipoxin A4 would prevent lung injury in a murine model of BPD.

Objective: To determine the effect of Resolvin D1 and/or Lipoxin A4 on hyperoxia-induced lung injury.

Methods: C57/BL6 pups were randomized at birth to Room Air, Hyperoxia (>90% oxygen), Hyperoxia + Resolvin D1, Hyperoxia + Lipoxin A4, or Hyperoxia + Resolvin D1/Lipoxin A4. Resolvin D1 and/or Lipoxin A4 (2 ng/g) were given IP on days 0, 3, 6, and 9. On day 10, mice were sacrificed and lungs collected for morphometric analyses including Mean Linear Intercept (MLI), Radial Alveolar Count (RAC), and Septal Thickness (ST); RT-PCR analyses of biomarkers of lung development and inflammation; and ELISA for TGFβ1 and TGFβ2.

Result: The increased ST observed with hyperoxia exposure was normalized by both Resolvin D1 and Lipoxin A4; while, hyperoxia-induced alveolar simplification was attenuated by Lipoxin A4. Relative to hyperoxia, Resolvin D1 reduced the gene expression of CXCL2 (2.9 fold), TIMP1 (6.7 fold), and PPARγ (4.8 fold). Treatment with Lipoxin A4 also led to a reduction of CXCL2 (2.4 fold) while selectively increasing TGFβ2 (2.1 fold) and Smad3 (1.58 fold).

Conclusion: The histologic and biochemical changes seen in hyperoxia-induced lung injury in this murine model can be reversed by the addition of DHA and AA fatty acid downstream metabolites that terminate the inflammatory pathways and modulate growth factors. These fatty acids or their metabolites may be novel therapies to prevent or treat lung injury in preterm infants.

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Related in: MedlinePlus

TGFβ2 and TGFβ1 ELISA.RA, room air; H, hyperoxia; RvD1, Resolvin D1; LXA4, Lipoxin A4.
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pone-0098773-g004: TGFβ2 and TGFβ1 ELISA.RA, room air; H, hyperoxia; RvD1, Resolvin D1; LXA4, Lipoxin A4.

Mentions: To evaluate further the RT-PCR changes found in TGFβ2 gene expression across the experimental groups, we performed an ELISA for TGFβ2. We also examined TGFβ1 to determine if the relative values seen in RT-PCR for both TGFβ2 and TGFβ1 parallel the changes in protein expression. The combined Hyperoxia + RvD1/LXA4 group had increased TGFβ2 protein levels (17.0 ± 1.3 ng/mg) compared to Hyperoxia alone (12.5 ± 0.7 ng/mg, p = 0.007) and Hyperoxia + RvD1 (13.1 ± 1.1 ng/mg, p = 0.02) (Figure 4). However, the overall ANOVA across all five experimental groups was short of statistical significance with a p-value of 0.08. For TGFβ1, the Hyperoxia + RvD1 group had decreased TGFβ1 protein levels (176.2 ± 12.5 ng/mg) compared to the RA group (217.0 ± 18.7 ng/mg, p = 0.04), however, the overall ANOVA across all groups was not significant (p = 0.3). These results demonstrate that the changes seen in mRNA expression in TGFβ2 and TGFβ1 parallel the relative changes seen in protein expression across the experimental groups.


Resolvin D1 and lipoxin A4 improve alveolarization and normalize septal wall thickness in a neonatal murine model of hyperoxia-induced lung injury.

Martin CR, Zaman MM, Gilkey C, Salguero MV, Hasturk H, Kantarci A, Van Dyke TE, Freedman SD - PLoS ONE (2014)

TGFβ2 and TGFβ1 ELISA.RA, room air; H, hyperoxia; RvD1, Resolvin D1; LXA4, Lipoxin A4.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4043836&req=5

pone-0098773-g004: TGFβ2 and TGFβ1 ELISA.RA, room air; H, hyperoxia; RvD1, Resolvin D1; LXA4, Lipoxin A4.
Mentions: To evaluate further the RT-PCR changes found in TGFβ2 gene expression across the experimental groups, we performed an ELISA for TGFβ2. We also examined TGFβ1 to determine if the relative values seen in RT-PCR for both TGFβ2 and TGFβ1 parallel the changes in protein expression. The combined Hyperoxia + RvD1/LXA4 group had increased TGFβ2 protein levels (17.0 ± 1.3 ng/mg) compared to Hyperoxia alone (12.5 ± 0.7 ng/mg, p = 0.007) and Hyperoxia + RvD1 (13.1 ± 1.1 ng/mg, p = 0.02) (Figure 4). However, the overall ANOVA across all five experimental groups was short of statistical significance with a p-value of 0.08. For TGFβ1, the Hyperoxia + RvD1 group had decreased TGFβ1 protein levels (176.2 ± 12.5 ng/mg) compared to the RA group (217.0 ± 18.7 ng/mg, p = 0.04), however, the overall ANOVA across all groups was not significant (p = 0.3). These results demonstrate that the changes seen in mRNA expression in TGFβ2 and TGFβ1 parallel the relative changes seen in protein expression across the experimental groups.

Bottom Line: To determine the effect of Resolvin D1 and/or Lipoxin A4 on hyperoxia-induced lung injury.Treatment with Lipoxin A4 also led to a reduction of CXCL2 (2.4 fold) while selectively increasing TGFβ2 (2.1 fold) and Smad3 (1.58 fold).These fatty acids or their metabolites may be novel therapies to prevent or treat lung injury in preterm infants.

View Article: PubMed Central - PubMed

Affiliation: Department of Neonatology, Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States of America; Division of Translational Research, Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States of America.

ABSTRACT

Background: The critical fatty acids Docosahexaenoic Acid (DHA) and Arachidonic Acid (AA) decline in preterm infants within the first postnatal week and are associated with neonatal morbidities, including bronchopulmonary dysplasia (BPD). DHA and AA are precursors to downstream metabolites that terminate the inflammatory response. We hypothesized that treatment with Resolvin D1 and/or Lipoxin A4 would prevent lung injury in a murine model of BPD.

Objective: To determine the effect of Resolvin D1 and/or Lipoxin A4 on hyperoxia-induced lung injury.

Methods: C57/BL6 pups were randomized at birth to Room Air, Hyperoxia (>90% oxygen), Hyperoxia + Resolvin D1, Hyperoxia + Lipoxin A4, or Hyperoxia + Resolvin D1/Lipoxin A4. Resolvin D1 and/or Lipoxin A4 (2 ng/g) were given IP on days 0, 3, 6, and 9. On day 10, mice were sacrificed and lungs collected for morphometric analyses including Mean Linear Intercept (MLI), Radial Alveolar Count (RAC), and Septal Thickness (ST); RT-PCR analyses of biomarkers of lung development and inflammation; and ELISA for TGFβ1 and TGFβ2.

Result: The increased ST observed with hyperoxia exposure was normalized by both Resolvin D1 and Lipoxin A4; while, hyperoxia-induced alveolar simplification was attenuated by Lipoxin A4. Relative to hyperoxia, Resolvin D1 reduced the gene expression of CXCL2 (2.9 fold), TIMP1 (6.7 fold), and PPARγ (4.8 fold). Treatment with Lipoxin A4 also led to a reduction of CXCL2 (2.4 fold) while selectively increasing TGFβ2 (2.1 fold) and Smad3 (1.58 fold).

Conclusion: The histologic and biochemical changes seen in hyperoxia-induced lung injury in this murine model can be reversed by the addition of DHA and AA fatty acid downstream metabolites that terminate the inflammatory pathways and modulate growth factors. These fatty acids or their metabolites may be novel therapies to prevent or treat lung injury in preterm infants.

Show MeSH
Related in: MedlinePlus