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Pneumococcal serotypes and serogroups causing invasive disease in Pakistan, 2005-2013.

Shakoor S, Kabir F, Khowaja AR, Qureshi SM, Jehan F, Qamar F, Whitney CG, Zaidi AK - PLoS ONE (2014)

Bottom Line: The 10-valent pneumococcal conjugate vaccine (PCV 10) or PCV10- related serotypes were found in 61% (n = 47) of childhood (age 0-15 years) meningitis episodes.PCV-13 increased this coverage to 63% (one additional serotype 19A; n = 48).Our data indicate that use of PCVs would prevent a large proportion of serious pneumococcal disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics and Child Health, the Aga Khan University Hospital, Karachi, Pakistan; Department of Pathology and Microbiology, the Aga Khan University Hospital, Karachi, Pakistan.

ABSTRACT
While pneumococcal conjugate vaccines have been implemented in most countries worldwide, use in Asia has lagged in part because of a lack of data on the amount of disease that is vaccine preventable in the region. We describe pneumococcal serotypes elicited from 111 episodes of invasive pneumococcal disease (IPD) from 2005 to 2013 among children and adults in Pakistan. Seventy-three percent (n = 81) of 111 IPD episodes were cases of meningitis (n = 76 in children 0-15 years and n = 5 among adults). Serotypes were determined by target amplification of DNA extracted from pneumococcal isolates (n = 52) or CSF specimens (n = 59). Serogroup 18 was the most common serogroup causing meningitis in children <5 years, accounting for 21% of cases (n = 13). The 10-valent pneumococcal conjugate vaccine (PCV 10) or PCV10- related serotypes were found in 61% (n = 47) of childhood (age 0-15 years) meningitis episodes. PCV-13 increased this coverage to 63% (one additional serotype 19A; n = 48). Our data indicate that use of PCVs would prevent a large proportion of serious pneumococcal disease.

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Related in: MedlinePlus

Vaccine coverage of pneumococcal serotypes in meningitis, Karachi and Hyderabad, Pakistan; 2009–2013 in children 0–59 months.(V =  Vaccine serotypes, VR =  Vaccine-related serotypes, and NV =  non-vaccine serotypes).
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pone-0098796-g001: Vaccine coverage of pneumococcal serotypes in meningitis, Karachi and Hyderabad, Pakistan; 2009–2013 in children 0–59 months.(V =  Vaccine serotypes, VR =  Vaccine-related serotypes, and NV =  non-vaccine serotypes).

Mentions: Serotyping by PCR was performed on 72 individual meningitis cases. Of these, 13 cases were taken from 2005–2006 (with archived pneumococcal isolates), and 59 cases were obtained from the Hib meningitis study [9]. The most common serogroup causing meningitis was serogroup 18 (n = 13), followed by serogroup 14 (n = 7), serotype 19F (n = 6), and serotype 23B (n = 6). The non-vaccine serotype 12F/A (detected as 12F/A/44/46) was found in 6 cases. Five cases remained non-typeable by PCR. These 5 were positive for lytA (detected in the CSF triplex) as well as for cpsA genes. Serotype data has been temporally disaggregated in Table 1 and Figure S1 in File S1. Figure 1 shows the proportion of strains covered by PCVs among children 0–59 months with meningitis seen from 2009 to 2013.


Pneumococcal serotypes and serogroups causing invasive disease in Pakistan, 2005-2013.

Shakoor S, Kabir F, Khowaja AR, Qureshi SM, Jehan F, Qamar F, Whitney CG, Zaidi AK - PLoS ONE (2014)

Vaccine coverage of pneumococcal serotypes in meningitis, Karachi and Hyderabad, Pakistan; 2009–2013 in children 0–59 months.(V =  Vaccine serotypes, VR =  Vaccine-related serotypes, and NV =  non-vaccine serotypes).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4043782&req=5

pone-0098796-g001: Vaccine coverage of pneumococcal serotypes in meningitis, Karachi and Hyderabad, Pakistan; 2009–2013 in children 0–59 months.(V =  Vaccine serotypes, VR =  Vaccine-related serotypes, and NV =  non-vaccine serotypes).
Mentions: Serotyping by PCR was performed on 72 individual meningitis cases. Of these, 13 cases were taken from 2005–2006 (with archived pneumococcal isolates), and 59 cases were obtained from the Hib meningitis study [9]. The most common serogroup causing meningitis was serogroup 18 (n = 13), followed by serogroup 14 (n = 7), serotype 19F (n = 6), and serotype 23B (n = 6). The non-vaccine serotype 12F/A (detected as 12F/A/44/46) was found in 6 cases. Five cases remained non-typeable by PCR. These 5 were positive for lytA (detected in the CSF triplex) as well as for cpsA genes. Serotype data has been temporally disaggregated in Table 1 and Figure S1 in File S1. Figure 1 shows the proportion of strains covered by PCVs among children 0–59 months with meningitis seen from 2009 to 2013.

Bottom Line: The 10-valent pneumococcal conjugate vaccine (PCV 10) or PCV10- related serotypes were found in 61% (n = 47) of childhood (age 0-15 years) meningitis episodes.PCV-13 increased this coverage to 63% (one additional serotype 19A; n = 48).Our data indicate that use of PCVs would prevent a large proportion of serious pneumococcal disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics and Child Health, the Aga Khan University Hospital, Karachi, Pakistan; Department of Pathology and Microbiology, the Aga Khan University Hospital, Karachi, Pakistan.

ABSTRACT
While pneumococcal conjugate vaccines have been implemented in most countries worldwide, use in Asia has lagged in part because of a lack of data on the amount of disease that is vaccine preventable in the region. We describe pneumococcal serotypes elicited from 111 episodes of invasive pneumococcal disease (IPD) from 2005 to 2013 among children and adults in Pakistan. Seventy-three percent (n = 81) of 111 IPD episodes were cases of meningitis (n = 76 in children 0-15 years and n = 5 among adults). Serotypes were determined by target amplification of DNA extracted from pneumococcal isolates (n = 52) or CSF specimens (n = 59). Serogroup 18 was the most common serogroup causing meningitis in children <5 years, accounting for 21% of cases (n = 13). The 10-valent pneumococcal conjugate vaccine (PCV 10) or PCV10- related serotypes were found in 61% (n = 47) of childhood (age 0-15 years) meningitis episodes. PCV-13 increased this coverage to 63% (one additional serotype 19A; n = 48). Our data indicate that use of PCVs would prevent a large proportion of serious pneumococcal disease.

Show MeSH
Related in: MedlinePlus