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A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil.

Giri AV, Anandkumar N, Muthukumaran G, Pennathur G - BMC Microbiol. (2004)

Bottom Line: The powdered peanut broth had supported better growth of Serratia marcescens and higher yield of prodigiosin when compared with the existing nutrient broth and peptone glycerol broth.From the results, the fatty acid form of carbon source has a role to play in enhanced cell growth and prodigiosin production.This work has also enabled us to report that the temperature related block of prodigiosin biosynthesis varies with different media and the powdered peanut broth supports prodigiosin production at higher temperatures.

View Article: PubMed Central - HTML - PubMed

Affiliation: Centre for Biotechnology, Anna University, Chennai 600025, India. anuvivek15@yahoo.com

ABSTRACT

Background: Prodigiosin produced by Serratia marcescens is a promising drug owing to its reported characteristics of having antifungal, immunosuppressive and antiproliferative activity. From an industrial point of view the necessity to obtain a suitable medium to simultaneously enhance the growth of Serratia marcescens and the pigment production was the aim of this work. The usage of individual fatty acid as substrate in industries would be cost-effective in the long run and this paved the way for us to try the effect of different fatty acid-containing seeds and oils of peanut, sesame and coconut as source of substrate.

Results: The addition of sugars only showed slight enhancement of prodigiosin production in nutrient broth but not in fatty acid containing seed medium. The powdered peanut broth had supported better growth of Serratia marcescens and higher yield of prodigiosin when compared with the existing nutrient broth and peptone glycerol broth. A block in prodigiosin production was seen above 30 degrees C in nutrient broth, but the fatty acid seed medium used by us supported prodigiosin production upto 42 degrees C though the yields were lower than what was obtained at 28 degrees C. From the results, the fatty acid form of carbon source has a role to play in enhanced cell growth and prodigiosin production.

Conclusion: We conclude by reporting that the powdered and sieved peanut seed of different quality grades were consistent in yielding a fourty fold increase in prodigiosin production over the existing media. A literature survey on the composition of the different media components in nutrient broth, peptone glycerol broth and the fatty acid containing seeds and oils enabled us to propose that the saturated form of fatty acid has a role to play in enhanced cell growth and prodigiosin production. This work has also enabled us to report that the temperature related block of prodigiosin biosynthesis varies with different media and the powdered peanut broth supports prodigiosin production at higher temperatures. The medium suggested in this work is best suitable from an industrial point of view in being economically feasible, in terms of the higher prodigiosin yield and the extraction of prodigiosin described in this paper is simple with minimal wastage.

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Growth of Serratia marcescens and prodigiosin production at different temperature in different media
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Figure 2: Growth of Serratia marcescens and prodigiosin production at different temperature in different media

Mentions: From a comparative study of Table 3 &4, the maximum prodigiosin production was seen at 28°C and 30°C in nutrient broth. At 37°C Serratia marcescens did not show any pigment production in nutrient broth and the culture broth was white in color. In case of the powdered peanut broth, even at 37°C, pigment production was observed and in fact it was equal to the amount of pigment production seen in nutrient broth at 30°C. In case of peanut broth only after second generation growth of Serratia marcescens at 42°C, there was complete block of pigment production as shown in Figure 2. Reversion of the 42°C grown white culture of Serratia marcescens which had shown pigment block in powdered peanut broth, showed the re-synthesis of pigment production when incubated at 28°C. Reversion experiment was done to confirm that at 42°C the culture was still viable and only the pigment production was blocked.


A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil.

Giri AV, Anandkumar N, Muthukumaran G, Pennathur G - BMC Microbiol. (2004)

Growth of Serratia marcescens and prodigiosin production at different temperature in different media
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC404375&req=5

Figure 2: Growth of Serratia marcescens and prodigiosin production at different temperature in different media
Mentions: From a comparative study of Table 3 &4, the maximum prodigiosin production was seen at 28°C and 30°C in nutrient broth. At 37°C Serratia marcescens did not show any pigment production in nutrient broth and the culture broth was white in color. In case of the powdered peanut broth, even at 37°C, pigment production was observed and in fact it was equal to the amount of pigment production seen in nutrient broth at 30°C. In case of peanut broth only after second generation growth of Serratia marcescens at 42°C, there was complete block of pigment production as shown in Figure 2. Reversion of the 42°C grown white culture of Serratia marcescens which had shown pigment block in powdered peanut broth, showed the re-synthesis of pigment production when incubated at 28°C. Reversion experiment was done to confirm that at 42°C the culture was still viable and only the pigment production was blocked.

Bottom Line: The powdered peanut broth had supported better growth of Serratia marcescens and higher yield of prodigiosin when compared with the existing nutrient broth and peptone glycerol broth.From the results, the fatty acid form of carbon source has a role to play in enhanced cell growth and prodigiosin production.This work has also enabled us to report that the temperature related block of prodigiosin biosynthesis varies with different media and the powdered peanut broth supports prodigiosin production at higher temperatures.

View Article: PubMed Central - HTML - PubMed

Affiliation: Centre for Biotechnology, Anna University, Chennai 600025, India. anuvivek15@yahoo.com

ABSTRACT

Background: Prodigiosin produced by Serratia marcescens is a promising drug owing to its reported characteristics of having antifungal, immunosuppressive and antiproliferative activity. From an industrial point of view the necessity to obtain a suitable medium to simultaneously enhance the growth of Serratia marcescens and the pigment production was the aim of this work. The usage of individual fatty acid as substrate in industries would be cost-effective in the long run and this paved the way for us to try the effect of different fatty acid-containing seeds and oils of peanut, sesame and coconut as source of substrate.

Results: The addition of sugars only showed slight enhancement of prodigiosin production in nutrient broth but not in fatty acid containing seed medium. The powdered peanut broth had supported better growth of Serratia marcescens and higher yield of prodigiosin when compared with the existing nutrient broth and peptone glycerol broth. A block in prodigiosin production was seen above 30 degrees C in nutrient broth, but the fatty acid seed medium used by us supported prodigiosin production upto 42 degrees C though the yields were lower than what was obtained at 28 degrees C. From the results, the fatty acid form of carbon source has a role to play in enhanced cell growth and prodigiosin production.

Conclusion: We conclude by reporting that the powdered and sieved peanut seed of different quality grades were consistent in yielding a fourty fold increase in prodigiosin production over the existing media. A literature survey on the composition of the different media components in nutrient broth, peptone glycerol broth and the fatty acid containing seeds and oils enabled us to propose that the saturated form of fatty acid has a role to play in enhanced cell growth and prodigiosin production. This work has also enabled us to report that the temperature related block of prodigiosin biosynthesis varies with different media and the powdered peanut broth supports prodigiosin production at higher temperatures. The medium suggested in this work is best suitable from an industrial point of view in being economically feasible, in terms of the higher prodigiosin yield and the extraction of prodigiosin described in this paper is simple with minimal wastage.

Show MeSH