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Enhanced antifungal efficacy of tebuconazole using gated pH-driven mesoporous nanoparticles.

Mas N, Galiana I, Hurtado S, Mondragón L, Bernardos A, Sancenón F, Marcos MD, Amorós P, Abril-Utrillas N, Martínez-Máñez R, Murguía JR - Int J Nanomedicine (2014)

Bottom Line: pH-sensitive gated mesoporous silica nanoparticles have been synthesized.Proper performance of the system was demonstrated with nanodevices loaded with fluorescein or with the antifungal agent tebuconazole.Interestingly, nanodevices loaded with tebuconazole significantly enhanced tebuconazole cytotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Centro Mixto Universidad Politécnica de Valencia, Universidad de Valencia, Valencia, Spain ; Departamento de Química, Universidad Politécnica de Valencia, Valencia, Spain ; CIBER de Bioingeniería, Biomateriales y Nanomedicina, Madrid, Spain.

ABSTRACT
pH-sensitive gated mesoporous silica nanoparticles have been synthesized. Increased extracellular pH and internalization into living yeast cells triggered molecular gate aperture and cargo release. Proper performance of the system was demonstrated with nanodevices loaded with fluorescein or with the antifungal agent tebuconazole. Interestingly, nanodevices loaded with tebuconazole significantly enhanced tebuconazole cytotoxicity. As alterations of acidic external pH are a key parameter in the onset of fungal vaginitis, this nanodevice could improve the treatment for vaginal mycoses.

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Related in: MedlinePlus

S1-Teb characterization results. (A) Nitrogen adsorption–desorption isotherms of MCM-41 calcined and S1-Teb. (B) Contents in mmol/g SiO2 for S1-Teb. Insets: Pore size distribution of MCM-41 calcined.Abbreviations: MCM-41, mobil composition of matter-41; STP, standard temperature and pressure; S1-Teb, MCM-41 loaded with tebuconazole.
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f3-ijn-9-2597: S1-Teb characterization results. (A) Nitrogen adsorption–desorption isotherms of MCM-41 calcined and S1-Teb. (B) Contents in mmol/g SiO2 for S1-Teb. Insets: Pore size distribution of MCM-41 calcined.Abbreviations: MCM-41, mobil composition of matter-41; STP, standard temperature and pressure; S1-Teb, MCM-41 loaded with tebuconazole.

Mentions: The nitrogen adsorption–desorption isotherms of the prepared phase (see Figure 3A) show a typical Pearson type IV curve with a specific surface area of 998.4 m2g−1, calculated with the BET38 model. The absence of a hysteresis loop in this interval and the narrow BJH39 pore size distribution, with an average pore diameter of 2.45 nm, indicates the existence of uniform cylindrical mesopores with a pore volume of 0.76 cm3g−1.


Enhanced antifungal efficacy of tebuconazole using gated pH-driven mesoporous nanoparticles.

Mas N, Galiana I, Hurtado S, Mondragón L, Bernardos A, Sancenón F, Marcos MD, Amorós P, Abril-Utrillas N, Martínez-Máñez R, Murguía JR - Int J Nanomedicine (2014)

S1-Teb characterization results. (A) Nitrogen adsorption–desorption isotherms of MCM-41 calcined and S1-Teb. (B) Contents in mmol/g SiO2 for S1-Teb. Insets: Pore size distribution of MCM-41 calcined.Abbreviations: MCM-41, mobil composition of matter-41; STP, standard temperature and pressure; S1-Teb, MCM-41 loaded with tebuconazole.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4043724&req=5

f3-ijn-9-2597: S1-Teb characterization results. (A) Nitrogen adsorption–desorption isotherms of MCM-41 calcined and S1-Teb. (B) Contents in mmol/g SiO2 for S1-Teb. Insets: Pore size distribution of MCM-41 calcined.Abbreviations: MCM-41, mobil composition of matter-41; STP, standard temperature and pressure; S1-Teb, MCM-41 loaded with tebuconazole.
Mentions: The nitrogen adsorption–desorption isotherms of the prepared phase (see Figure 3A) show a typical Pearson type IV curve with a specific surface area of 998.4 m2g−1, calculated with the BET38 model. The absence of a hysteresis loop in this interval and the narrow BJH39 pore size distribution, with an average pore diameter of 2.45 nm, indicates the existence of uniform cylindrical mesopores with a pore volume of 0.76 cm3g−1.

Bottom Line: pH-sensitive gated mesoporous silica nanoparticles have been synthesized.Proper performance of the system was demonstrated with nanodevices loaded with fluorescein or with the antifungal agent tebuconazole.Interestingly, nanodevices loaded with tebuconazole significantly enhanced tebuconazole cytotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Centro Mixto Universidad Politécnica de Valencia, Universidad de Valencia, Valencia, Spain ; Departamento de Química, Universidad Politécnica de Valencia, Valencia, Spain ; CIBER de Bioingeniería, Biomateriales y Nanomedicina, Madrid, Spain.

ABSTRACT
pH-sensitive gated mesoporous silica nanoparticles have been synthesized. Increased extracellular pH and internalization into living yeast cells triggered molecular gate aperture and cargo release. Proper performance of the system was demonstrated with nanodevices loaded with fluorescein or with the antifungal agent tebuconazole. Interestingly, nanodevices loaded with tebuconazole significantly enhanced tebuconazole cytotoxicity. As alterations of acidic external pH are a key parameter in the onset of fungal vaginitis, this nanodevice could improve the treatment for vaginal mycoses.

Show MeSH
Related in: MedlinePlus