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Mesoporous magnesium silicate-incorporated poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) bioactive composite beneficial to osteoblast behaviors.

Niu Y, Dong W, Guo H, Deng Y, Guo L, An X, He D, Wei J, Li M - Int J Nanomedicine (2014)

Bottom Line: The results suggest that the mechanical properties of compressive strength and elastic modulus, as well as hydrophilicity, of the m-MPC increased with increase of m-MS content in the composites.In cell cultural experiments, the results showed that the attachment and viability ratio of MG63 cells on m-MPC increased significantly with the increase of m-MS content, showing that the addition of m-MS into PCL-PEG-PCL could promote cell attachment and proliferation.The results suggest that the incorporation of m-MS into PCL-PEG-PCL could produce bioactive composites with improved hydrophilicity, degradability, bioactivity, and cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.

ABSTRACT
Mesoporous magnesium silicate (m-MS) and poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL) composite (m-MPC) was synthesized by solvent casting method. The results suggest that the mechanical properties of compressive strength and elastic modulus, as well as hydrophilicity, of the m-MPC increased with increase of m-MS content in the composites. In addition, the weight loss of the m-MPC improved significantly with the increase of m-MS content during composite soaking in phosphate-buffered saline for 10 weeks, indicating that incorporation of m-MS into PCL-PEG-PCL could enhance the degradability of the m-MPC. Moreover, the m-MPC with 40 w% m-MS could induce a dense and continuous apatite layer on its surface after soaking in simulated body fluid for 5 days, which was better than m-MPC 20 w% m-MS, exhibiting excellent in vitro bioactivity. In cell cultural experiments, the results showed that the attachment and viability ratio of MG63 cells on m-MPC increased significantly with the increase of m-MS content, showing that the addition of m-MS into PCL-PEG-PCL could promote cell attachment and proliferation. The results suggest that the incorporation of m-MS into PCL-PEG-PCL could produce bioactive composites with improved hydrophilicity, degradability, bioactivity, and cytocompatibility.

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Proliferation of MG63 cells on m-MPC with 20 w% and 40 w% m-MS and on PCL-PEG-PCL (control).Note: *Significant difference (P<0.05).Abbreviations: m-MPC, m-MS and PCL-PEG-PCL composite; m-MS, mesoporous magnesium silicate; OD, optical density; PCL-PEG-PCL, poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone).
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f9-ijn-9-2665: Proliferation of MG63 cells on m-MPC with 20 w% and 40 w% m-MS and on PCL-PEG-PCL (control).Note: *Significant difference (P<0.05).Abbreviations: m-MPC, m-MS and PCL-PEG-PCL composite; m-MS, mesoporous magnesium silicate; OD, optical density; PCL-PEG-PCL, poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone).

Mentions: Proliferation of MG63 cells cultured on the m-MPC was assessed using the MTT assay because OD values could provide an indication of cell growth. Figure 9 reveals that the OD value for all the samples increased with time, and no significant differences were found among the samples at day 1. The OD values for m-MPC with 20 w% and 40 w% m-MS were significantly higher than that of PCL-PEG-PCL at 4 and 7 days (P<0.05); no significant differences appeared between m-MPC with 20 w% and 40 w% m-MS at 4 and 7 days. The results indicate that cell growth and viability in m-MPC were superior to those in PCL-PEG-PCL, suggesting that m-MPC facilitates cell growth and can promote cell viability.


Mesoporous magnesium silicate-incorporated poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) bioactive composite beneficial to osteoblast behaviors.

Niu Y, Dong W, Guo H, Deng Y, Guo L, An X, He D, Wei J, Li M - Int J Nanomedicine (2014)

Proliferation of MG63 cells on m-MPC with 20 w% and 40 w% m-MS and on PCL-PEG-PCL (control).Note: *Significant difference (P<0.05).Abbreviations: m-MPC, m-MS and PCL-PEG-PCL composite; m-MS, mesoporous magnesium silicate; OD, optical density; PCL-PEG-PCL, poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4043715&req=5

f9-ijn-9-2665: Proliferation of MG63 cells on m-MPC with 20 w% and 40 w% m-MS and on PCL-PEG-PCL (control).Note: *Significant difference (P<0.05).Abbreviations: m-MPC, m-MS and PCL-PEG-PCL composite; m-MS, mesoporous magnesium silicate; OD, optical density; PCL-PEG-PCL, poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone).
Mentions: Proliferation of MG63 cells cultured on the m-MPC was assessed using the MTT assay because OD values could provide an indication of cell growth. Figure 9 reveals that the OD value for all the samples increased with time, and no significant differences were found among the samples at day 1. The OD values for m-MPC with 20 w% and 40 w% m-MS were significantly higher than that of PCL-PEG-PCL at 4 and 7 days (P<0.05); no significant differences appeared between m-MPC with 20 w% and 40 w% m-MS at 4 and 7 days. The results indicate that cell growth and viability in m-MPC were superior to those in PCL-PEG-PCL, suggesting that m-MPC facilitates cell growth and can promote cell viability.

Bottom Line: The results suggest that the mechanical properties of compressive strength and elastic modulus, as well as hydrophilicity, of the m-MPC increased with increase of m-MS content in the composites.In cell cultural experiments, the results showed that the attachment and viability ratio of MG63 cells on m-MPC increased significantly with the increase of m-MS content, showing that the addition of m-MS into PCL-PEG-PCL could promote cell attachment and proliferation.The results suggest that the incorporation of m-MS into PCL-PEG-PCL could produce bioactive composites with improved hydrophilicity, degradability, bioactivity, and cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.

ABSTRACT
Mesoporous magnesium silicate (m-MS) and poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL) composite (m-MPC) was synthesized by solvent casting method. The results suggest that the mechanical properties of compressive strength and elastic modulus, as well as hydrophilicity, of the m-MPC increased with increase of m-MS content in the composites. In addition, the weight loss of the m-MPC improved significantly with the increase of m-MS content during composite soaking in phosphate-buffered saline for 10 weeks, indicating that incorporation of m-MS into PCL-PEG-PCL could enhance the degradability of the m-MPC. Moreover, the m-MPC with 40 w% m-MS could induce a dense and continuous apatite layer on its surface after soaking in simulated body fluid for 5 days, which was better than m-MPC 20 w% m-MS, exhibiting excellent in vitro bioactivity. In cell cultural experiments, the results showed that the attachment and viability ratio of MG63 cells on m-MPC increased significantly with the increase of m-MS content, showing that the addition of m-MS into PCL-PEG-PCL could promote cell attachment and proliferation. The results suggest that the incorporation of m-MS into PCL-PEG-PCL could produce bioactive composites with improved hydrophilicity, degradability, bioactivity, and cytocompatibility.

Show MeSH
Related in: MedlinePlus