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Mesoporous magnesium silicate-incorporated poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) bioactive composite beneficial to osteoblast behaviors.

Niu Y, Dong W, Guo H, Deng Y, Guo L, An X, He D, Wei J, Li M - Int J Nanomedicine (2014)

Bottom Line: The results suggest that the mechanical properties of compressive strength and elastic modulus, as well as hydrophilicity, of the m-MPC increased with increase of m-MS content in the composites.In cell cultural experiments, the results showed that the attachment and viability ratio of MG63 cells on m-MPC increased significantly with the increase of m-MS content, showing that the addition of m-MS into PCL-PEG-PCL could promote cell attachment and proliferation.The results suggest that the incorporation of m-MS into PCL-PEG-PCL could produce bioactive composites with improved hydrophilicity, degradability, bioactivity, and cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.

ABSTRACT
Mesoporous magnesium silicate (m-MS) and poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL) composite (m-MPC) was synthesized by solvent casting method. The results suggest that the mechanical properties of compressive strength and elastic modulus, as well as hydrophilicity, of the m-MPC increased with increase of m-MS content in the composites. In addition, the weight loss of the m-MPC improved significantly with the increase of m-MS content during composite soaking in phosphate-buffered saline for 10 weeks, indicating that incorporation of m-MS into PCL-PEG-PCL could enhance the degradability of the m-MPC. Moreover, the m-MPC with 40 w% m-MS could induce a dense and continuous apatite layer on its surface after soaking in simulated body fluid for 5 days, which was better than m-MPC 20 w% m-MS, exhibiting excellent in vitro bioactivity. In cell cultural experiments, the results showed that the attachment and viability ratio of MG63 cells on m-MPC increased significantly with the increase of m-MS content, showing that the addition of m-MS into PCL-PEG-PCL could promote cell attachment and proliferation. The results suggest that the incorporation of m-MS into PCL-PEG-PCL could produce bioactive composites with improved hydrophilicity, degradability, bioactivity, and cytocompatibility.

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MG63 cell adhesion on m-MPC with 20 w% and 40 w% m-MS, and on PCL-PEG-PCL and TCP (controls).Note: *Significant difference (P<0.05).Abbreviations: m-MPC, m-MS and PCL-PEG-PCL composite; m-MS, mesoporous magnesium silicate; PCL-PEG-PCL, poly(ε-caprolactone)-poly(ethylene glycol)-poly (ε-caprolactone); TCP, tissue culture plate.
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f8-ijn-9-2665: MG63 cell adhesion on m-MPC with 20 w% and 40 w% m-MS, and on PCL-PEG-PCL and TCP (controls).Note: *Significant difference (P<0.05).Abbreviations: m-MPC, m-MS and PCL-PEG-PCL composite; m-MS, mesoporous magnesium silicate; PCL-PEG-PCL, poly(ε-caprolactone)-poly(ethylene glycol)-poly (ε-caprolactone); TCP, tissue culture plate.

Mentions: Cell attachment was assessed using MG63 cells cultured on m-MPC with 0 w%, 20 w%, and 40 w% m-MS, and TCP was used as a control. The results of cell attachment efficiency are profiled in Figure 8. In a period of 4 hours, it was found that the cell attachment ratio of the m-MPC with 40 w% m-MS (131%) was significantly higher than that of m-MPC with 20 w% m-MS (114%). Moreover, the cell attachment ratio of the PCL-PEG-PCL was only 91%, which was lower than that of the TCP control (100%). The results show improved cell attachment relative to the m-MPC with higher m-MS content, suggesting that the addition of m-MS into PCL-PEG-PCL facilitated cell adhesion.


Mesoporous magnesium silicate-incorporated poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) bioactive composite beneficial to osteoblast behaviors.

Niu Y, Dong W, Guo H, Deng Y, Guo L, An X, He D, Wei J, Li M - Int J Nanomedicine (2014)

MG63 cell adhesion on m-MPC with 20 w% and 40 w% m-MS, and on PCL-PEG-PCL and TCP (controls).Note: *Significant difference (P<0.05).Abbreviations: m-MPC, m-MS and PCL-PEG-PCL composite; m-MS, mesoporous magnesium silicate; PCL-PEG-PCL, poly(ε-caprolactone)-poly(ethylene glycol)-poly (ε-caprolactone); TCP, tissue culture plate.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4043715&req=5

f8-ijn-9-2665: MG63 cell adhesion on m-MPC with 20 w% and 40 w% m-MS, and on PCL-PEG-PCL and TCP (controls).Note: *Significant difference (P<0.05).Abbreviations: m-MPC, m-MS and PCL-PEG-PCL composite; m-MS, mesoporous magnesium silicate; PCL-PEG-PCL, poly(ε-caprolactone)-poly(ethylene glycol)-poly (ε-caprolactone); TCP, tissue culture plate.
Mentions: Cell attachment was assessed using MG63 cells cultured on m-MPC with 0 w%, 20 w%, and 40 w% m-MS, and TCP was used as a control. The results of cell attachment efficiency are profiled in Figure 8. In a period of 4 hours, it was found that the cell attachment ratio of the m-MPC with 40 w% m-MS (131%) was significantly higher than that of m-MPC with 20 w% m-MS (114%). Moreover, the cell attachment ratio of the PCL-PEG-PCL was only 91%, which was lower than that of the TCP control (100%). The results show improved cell attachment relative to the m-MPC with higher m-MS content, suggesting that the addition of m-MS into PCL-PEG-PCL facilitated cell adhesion.

Bottom Line: The results suggest that the mechanical properties of compressive strength and elastic modulus, as well as hydrophilicity, of the m-MPC increased with increase of m-MS content in the composites.In cell cultural experiments, the results showed that the attachment and viability ratio of MG63 cells on m-MPC increased significantly with the increase of m-MS content, showing that the addition of m-MS into PCL-PEG-PCL could promote cell attachment and proliferation.The results suggest that the incorporation of m-MS into PCL-PEG-PCL could produce bioactive composites with improved hydrophilicity, degradability, bioactivity, and cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.

ABSTRACT
Mesoporous magnesium silicate (m-MS) and poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL) composite (m-MPC) was synthesized by solvent casting method. The results suggest that the mechanical properties of compressive strength and elastic modulus, as well as hydrophilicity, of the m-MPC increased with increase of m-MS content in the composites. In addition, the weight loss of the m-MPC improved significantly with the increase of m-MS content during composite soaking in phosphate-buffered saline for 10 weeks, indicating that incorporation of m-MS into PCL-PEG-PCL could enhance the degradability of the m-MPC. Moreover, the m-MPC with 40 w% m-MS could induce a dense and continuous apatite layer on its surface after soaking in simulated body fluid for 5 days, which was better than m-MPC 20 w% m-MS, exhibiting excellent in vitro bioactivity. In cell cultural experiments, the results showed that the attachment and viability ratio of MG63 cells on m-MPC increased significantly with the increase of m-MS content, showing that the addition of m-MS into PCL-PEG-PCL could promote cell attachment and proliferation. The results suggest that the incorporation of m-MS into PCL-PEG-PCL could produce bioactive composites with improved hydrophilicity, degradability, bioactivity, and cytocompatibility.

Show MeSH
Related in: MedlinePlus