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Mesoporous magnesium silicate-incorporated poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) bioactive composite beneficial to osteoblast behaviors.

Niu Y, Dong W, Guo H, Deng Y, Guo L, An X, He D, Wei J, Li M - Int J Nanomedicine (2014)

Bottom Line: The results suggest that the mechanical properties of compressive strength and elastic modulus, as well as hydrophilicity, of the m-MPC increased with increase of m-MS content in the composites.In cell cultural experiments, the results showed that the attachment and viability ratio of MG63 cells on m-MPC increased significantly with the increase of m-MS content, showing that the addition of m-MS into PCL-PEG-PCL could promote cell attachment and proliferation.The results suggest that the incorporation of m-MS into PCL-PEG-PCL could produce bioactive composites with improved hydrophilicity, degradability, bioactivity, and cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.

ABSTRACT
Mesoporous magnesium silicate (m-MS) and poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL) composite (m-MPC) was synthesized by solvent casting method. The results suggest that the mechanical properties of compressive strength and elastic modulus, as well as hydrophilicity, of the m-MPC increased with increase of m-MS content in the composites. In addition, the weight loss of the m-MPC improved significantly with the increase of m-MS content during composite soaking in phosphate-buffered saline for 10 weeks, indicating that incorporation of m-MS into PCL-PEG-PCL could enhance the degradability of the m-MPC. Moreover, the m-MPC with 40 w% m-MS could induce a dense and continuous apatite layer on its surface after soaking in simulated body fluid for 5 days, which was better than m-MPC 20 w% m-MS, exhibiting excellent in vitro bioactivity. In cell cultural experiments, the results showed that the attachment and viability ratio of MG63 cells on m-MPC increased significantly with the increase of m-MS content, showing that the addition of m-MS into PCL-PEG-PCL could promote cell attachment and proliferation. The results suggest that the incorporation of m-MS into PCL-PEG-PCL could produce bioactive composites with improved hydrophilicity, degradability, bioactivity, and cytocompatibility.

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Scanning electron microscope images of PCL-PEG-PCL (A) and m-MPC with 20 w% (B) and 40 w% (C) m-MS after being soaked in simulated body fluid for 5 days, and EDS of m-MPC with 40 w% m-MS after being soaked in simulated body fluid for 5 days (D).Abbreviations: au, arbitrary units; m-MPC, m-MS and PCL-PEG-PCL composite; m-MS, mesoporous magnesium silicate; PCL-PEG-PCL, poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone).
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f6-ijn-9-2665: Scanning electron microscope images of PCL-PEG-PCL (A) and m-MPC with 20 w% (B) and 40 w% (C) m-MS after being soaked in simulated body fluid for 5 days, and EDS of m-MPC with 40 w% m-MS after being soaked in simulated body fluid for 5 days (D).Abbreviations: au, arbitrary units; m-MPC, m-MS and PCL-PEG-PCL composite; m-MS, mesoporous magnesium silicate; PCL-PEG-PCL, poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone).

Mentions: After soaking in SBF for 5 days, it was found that spherical apatite granules were formed on the surfaces of m-MPC with 20 w% and 40 w% m-MS. No apatite formation was found on the PCL-PEG-PCL surface (Figure 6A). Only a few spherical apatite granules were found on the m-MPC surface with 20 w% m-MS (Figure 6B), while spherical granules were found in a densely packed apatite layer on m-MPC with 40 w% m-MS (Figure 6C). The EDS spectra were used to examine the apatite formation on the surface of m-MPC with 20 w% and 40 w% m-MS after soaking in SBF for 5 days (Figure 6D). Calcium and phosphorous peaks were detected and the calcium/phosphorous ratios of the apatites on the m-MPC with 20 w% and 40 w% m-MS were found to be 1.5 and 1.52, respectively, which were both less than the calcium/phosphorous ratio of 1.67 in hydroxyapatite. This indicates that the apatite formation on m-MPC was calcium deficient.


Mesoporous magnesium silicate-incorporated poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) bioactive composite beneficial to osteoblast behaviors.

Niu Y, Dong W, Guo H, Deng Y, Guo L, An X, He D, Wei J, Li M - Int J Nanomedicine (2014)

Scanning electron microscope images of PCL-PEG-PCL (A) and m-MPC with 20 w% (B) and 40 w% (C) m-MS after being soaked in simulated body fluid for 5 days, and EDS of m-MPC with 40 w% m-MS after being soaked in simulated body fluid for 5 days (D).Abbreviations: au, arbitrary units; m-MPC, m-MS and PCL-PEG-PCL composite; m-MS, mesoporous magnesium silicate; PCL-PEG-PCL, poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4043715&req=5

f6-ijn-9-2665: Scanning electron microscope images of PCL-PEG-PCL (A) and m-MPC with 20 w% (B) and 40 w% (C) m-MS after being soaked in simulated body fluid for 5 days, and EDS of m-MPC with 40 w% m-MS after being soaked in simulated body fluid for 5 days (D).Abbreviations: au, arbitrary units; m-MPC, m-MS and PCL-PEG-PCL composite; m-MS, mesoporous magnesium silicate; PCL-PEG-PCL, poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone).
Mentions: After soaking in SBF for 5 days, it was found that spherical apatite granules were formed on the surfaces of m-MPC with 20 w% and 40 w% m-MS. No apatite formation was found on the PCL-PEG-PCL surface (Figure 6A). Only a few spherical apatite granules were found on the m-MPC surface with 20 w% m-MS (Figure 6B), while spherical granules were found in a densely packed apatite layer on m-MPC with 40 w% m-MS (Figure 6C). The EDS spectra were used to examine the apatite formation on the surface of m-MPC with 20 w% and 40 w% m-MS after soaking in SBF for 5 days (Figure 6D). Calcium and phosphorous peaks were detected and the calcium/phosphorous ratios of the apatites on the m-MPC with 20 w% and 40 w% m-MS were found to be 1.5 and 1.52, respectively, which were both less than the calcium/phosphorous ratio of 1.67 in hydroxyapatite. This indicates that the apatite formation on m-MPC was calcium deficient.

Bottom Line: The results suggest that the mechanical properties of compressive strength and elastic modulus, as well as hydrophilicity, of the m-MPC increased with increase of m-MS content in the composites.In cell cultural experiments, the results showed that the attachment and viability ratio of MG63 cells on m-MPC increased significantly with the increase of m-MS content, showing that the addition of m-MS into PCL-PEG-PCL could promote cell attachment and proliferation.The results suggest that the incorporation of m-MS into PCL-PEG-PCL could produce bioactive composites with improved hydrophilicity, degradability, bioactivity, and cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.

ABSTRACT
Mesoporous magnesium silicate (m-MS) and poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL) composite (m-MPC) was synthesized by solvent casting method. The results suggest that the mechanical properties of compressive strength and elastic modulus, as well as hydrophilicity, of the m-MPC increased with increase of m-MS content in the composites. In addition, the weight loss of the m-MPC improved significantly with the increase of m-MS content during composite soaking in phosphate-buffered saline for 10 weeks, indicating that incorporation of m-MS into PCL-PEG-PCL could enhance the degradability of the m-MPC. Moreover, the m-MPC with 40 w% m-MS could induce a dense and continuous apatite layer on its surface after soaking in simulated body fluid for 5 days, which was better than m-MPC 20 w% m-MS, exhibiting excellent in vitro bioactivity. In cell cultural experiments, the results showed that the attachment and viability ratio of MG63 cells on m-MPC increased significantly with the increase of m-MS content, showing that the addition of m-MS into PCL-PEG-PCL could promote cell attachment and proliferation. The results suggest that the incorporation of m-MS into PCL-PEG-PCL could produce bioactive composites with improved hydrophilicity, degradability, bioactivity, and cytocompatibility.

Show MeSH
Related in: MedlinePlus