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Rapid efficient synthesis and characterization of silver, gold, and bimetallic nanoparticles from the medicinal plant Plumbago zeylanica and their application in biofilm control.

Salunke GR, Ghosh S, Santosh Kumar RJ, Khade S, Vashisth P, Kale T, Chopade S, Pruthi V, Kundu G, Bellare JR, Chopade BA - Int J Nanomedicine (2014)

Bottom Line: The synthesis of AgAuNPs, which completed within 90 minutes with 0.7 mM AgNO₃ and HAuCl₄, was found to be the fastest.Fourier-transform infrared spectroscopy confirmed bioreduction, while EDS and XRD patterns confirmed purity and the crystalline nature of the NPs, respectively.This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms.

View Article: PubMed Central - PubMed

Affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, India.

ABSTRACT

Background: Nanoparticles (NPs) have gained significance in medical fields due to their high surface-area-to-volume ratio. In this study, we synthesized NPs from a medicinally important plant - Plumbago zeylanica.

Materials and methods: Aqueous root extract of P. zeylanica (PZRE) was analyzed for the presence of flavonoids, sugars, and organic acids using high-performance thin-layer chromatography (HPTLC), gas chromatography-time of flight-mass spectrometry (GC-TOF-MS), and biochemical methods. The silver NPs (AgNPs), gold NPs (AuNPs), and bimetallic NPs (AgAuNPs) were synthesized from root extract and characterized using ultraviolet-visible spectra, X-ray diffraction (XRD), energy-dispersive spectrometry (EDS), transmission electron microscopy (TEM), and dynamic light scattering (DLS). The effects of these NPs on Acinetobacter baumannii, Staphylococcus aureus, and Escherichia coli biofilms were studied using quantitative biofilm inhibition and disruption assays, as well as using fluorescence, scanning electron microscopy, and atomic force microscopy.

Results: PZRE showed the presence of phenolics, such as plumbagin, and flavonoids, in addition to citric acid, sucrose, glucose, fructose, and starch, using HPTLC, GC-TOF-MS, and quantitative analysis. Bioreduction of silver nitrate (AgNO₃) and chloroauric acid (HAuCl₄) were confirmed at absorbances of 440 nm (AgNPs), 570 nm (AuNPs), and 540 nm (AgAuNPs), respectively. The maximum rate of synthesis at 50°C was achieved with 5 mM AgNO₃ within 4.5 hours for AgNPs; and with 0.7 mM HAuCl4 within 5 hours for AuNPs. The synthesis of AgAuNPs, which completed within 90 minutes with 0.7 mM AgNO₃ and HAuCl₄, was found to be the fastest. Fourier-transform infrared spectroscopy confirmed bioreduction, while EDS and XRD patterns confirmed purity and the crystalline nature of the NPs, respectively. TEM micrographs and DLS showed about 60 nm monodispersed Ag nanospheres, 20-30 nm Au nanospheres adhering to form Au nanotriangles, and about 90 nm hexagonal blunt-ended AgAuNPs. These NPs also showed antimicrobial and antibiofilm activity against E. coli, A. baumannii, S. aureus, and a mixed culture of A. baumannii and S. aureus. AgNPs inhibited biofilm in the range of 96%-99% and AgAuNPs from 93% to 98% in single-culture biofilms. AuNPs also showed biofilm inhibition, with the highest of 98% in S. aureus. AgNPs also showed good biofilm disruption, with the highest of 88% in A. baumannii.

Conclusion: This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms.

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Inhibition and disruption of bacterial biofilms by biologically and chemically synthesized nanoparticles at a concentration of 1,024 μg/well at 24 hours. (A) Biofilm inhibition; (B) biofilm disruption.Abbreviations:A. baumannii, Acinetobacter baumannii; E. coli, Escherichia coli; S. aureus, Staphylococcus aureus; AgNPs, silver nanoparticles; AuNPs, gold NPs; AgAuNPs, bimetallic NPs; chem, chemically synthesized.
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f7-ijn-9-2635: Inhibition and disruption of bacterial biofilms by biologically and chemically synthesized nanoparticles at a concentration of 1,024 μg/well at 24 hours. (A) Biofilm inhibition; (B) biofilm disruption.Abbreviations:A. baumannii, Acinetobacter baumannii; E. coli, Escherichia coli; S. aureus, Staphylococcus aureus; AgNPs, silver nanoparticles; AuNPs, gold NPs; AgAuNPs, bimetallic NPs; chem, chemically synthesized.

Mentions: As all three NP types – AgNPs, AuNPs, and AgAuNPs – showed superior antibacterial activity, these NPs were further studied for biofilm inhibition and biofilm disruption with chemically synthesized NPs as a control. Biofilm inhibition with NP concentration of 1,024 μg/well after 24 hours of coincubation is represented by the graph in Figure 7A. It was observed that AgNPs and AgAuNPs inhibited the biofilm in all the test-microorganism single cultures around 96%–99% and 93%–98%, respectively. This was interesting compared to the control AgNPs and AgAuNPs, which showed inhibition of 67%–91% and 0%–82%, respectively. In the case of mixed culture, chemically synthesized AgNPs did not inhibit biofilm formation, whereas AgNPs from PZRE showed 91% biofilm inhibition. AgAuNPs showed A. baumannii biofilm inhibition up to 94% and E. coli biofilm inhibition up to 98%, with the highest inhibition of S. aureus biofilm of 99%. Similar to control AgNPs, control AgAuNPs showed poor biofilm inhibition compared to biological AgAuNPs, whereas AuNPs from both biological and chemical sources showed poor biofilm inhibition and disruption, except AuNPs from PZRE, which showed 97% S. aureus biofilm inhibition.


Rapid efficient synthesis and characterization of silver, gold, and bimetallic nanoparticles from the medicinal plant Plumbago zeylanica and their application in biofilm control.

Salunke GR, Ghosh S, Santosh Kumar RJ, Khade S, Vashisth P, Kale T, Chopade S, Pruthi V, Kundu G, Bellare JR, Chopade BA - Int J Nanomedicine (2014)

Inhibition and disruption of bacterial biofilms by biologically and chemically synthesized nanoparticles at a concentration of 1,024 μg/well at 24 hours. (A) Biofilm inhibition; (B) biofilm disruption.Abbreviations:A. baumannii, Acinetobacter baumannii; E. coli, Escherichia coli; S. aureus, Staphylococcus aureus; AgNPs, silver nanoparticles; AuNPs, gold NPs; AgAuNPs, bimetallic NPs; chem, chemically synthesized.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4043712&req=5

f7-ijn-9-2635: Inhibition and disruption of bacterial biofilms by biologically and chemically synthesized nanoparticles at a concentration of 1,024 μg/well at 24 hours. (A) Biofilm inhibition; (B) biofilm disruption.Abbreviations:A. baumannii, Acinetobacter baumannii; E. coli, Escherichia coli; S. aureus, Staphylococcus aureus; AgNPs, silver nanoparticles; AuNPs, gold NPs; AgAuNPs, bimetallic NPs; chem, chemically synthesized.
Mentions: As all three NP types – AgNPs, AuNPs, and AgAuNPs – showed superior antibacterial activity, these NPs were further studied for biofilm inhibition and biofilm disruption with chemically synthesized NPs as a control. Biofilm inhibition with NP concentration of 1,024 μg/well after 24 hours of coincubation is represented by the graph in Figure 7A. It was observed that AgNPs and AgAuNPs inhibited the biofilm in all the test-microorganism single cultures around 96%–99% and 93%–98%, respectively. This was interesting compared to the control AgNPs and AgAuNPs, which showed inhibition of 67%–91% and 0%–82%, respectively. In the case of mixed culture, chemically synthesized AgNPs did not inhibit biofilm formation, whereas AgNPs from PZRE showed 91% biofilm inhibition. AgAuNPs showed A. baumannii biofilm inhibition up to 94% and E. coli biofilm inhibition up to 98%, with the highest inhibition of S. aureus biofilm of 99%. Similar to control AgNPs, control AgAuNPs showed poor biofilm inhibition compared to biological AgAuNPs, whereas AuNPs from both biological and chemical sources showed poor biofilm inhibition and disruption, except AuNPs from PZRE, which showed 97% S. aureus biofilm inhibition.

Bottom Line: The synthesis of AgAuNPs, which completed within 90 minutes with 0.7 mM AgNO₃ and HAuCl₄, was found to be the fastest.Fourier-transform infrared spectroscopy confirmed bioreduction, while EDS and XRD patterns confirmed purity and the crystalline nature of the NPs, respectively.This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms.

View Article: PubMed Central - PubMed

Affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, India.

ABSTRACT

Background: Nanoparticles (NPs) have gained significance in medical fields due to their high surface-area-to-volume ratio. In this study, we synthesized NPs from a medicinally important plant - Plumbago zeylanica.

Materials and methods: Aqueous root extract of P. zeylanica (PZRE) was analyzed for the presence of flavonoids, sugars, and organic acids using high-performance thin-layer chromatography (HPTLC), gas chromatography-time of flight-mass spectrometry (GC-TOF-MS), and biochemical methods. The silver NPs (AgNPs), gold NPs (AuNPs), and bimetallic NPs (AgAuNPs) were synthesized from root extract and characterized using ultraviolet-visible spectra, X-ray diffraction (XRD), energy-dispersive spectrometry (EDS), transmission electron microscopy (TEM), and dynamic light scattering (DLS). The effects of these NPs on Acinetobacter baumannii, Staphylococcus aureus, and Escherichia coli biofilms were studied using quantitative biofilm inhibition and disruption assays, as well as using fluorescence, scanning electron microscopy, and atomic force microscopy.

Results: PZRE showed the presence of phenolics, such as plumbagin, and flavonoids, in addition to citric acid, sucrose, glucose, fructose, and starch, using HPTLC, GC-TOF-MS, and quantitative analysis. Bioreduction of silver nitrate (AgNO₃) and chloroauric acid (HAuCl₄) were confirmed at absorbances of 440 nm (AgNPs), 570 nm (AuNPs), and 540 nm (AgAuNPs), respectively. The maximum rate of synthesis at 50°C was achieved with 5 mM AgNO₃ within 4.5 hours for AgNPs; and with 0.7 mM HAuCl4 within 5 hours for AuNPs. The synthesis of AgAuNPs, which completed within 90 minutes with 0.7 mM AgNO₃ and HAuCl₄, was found to be the fastest. Fourier-transform infrared spectroscopy confirmed bioreduction, while EDS and XRD patterns confirmed purity and the crystalline nature of the NPs, respectively. TEM micrographs and DLS showed about 60 nm monodispersed Ag nanospheres, 20-30 nm Au nanospheres adhering to form Au nanotriangles, and about 90 nm hexagonal blunt-ended AgAuNPs. These NPs also showed antimicrobial and antibiofilm activity against E. coli, A. baumannii, S. aureus, and a mixed culture of A. baumannii and S. aureus. AgNPs inhibited biofilm in the range of 96%-99% and AgAuNPs from 93% to 98% in single-culture biofilms. AuNPs also showed biofilm inhibition, with the highest of 98% in S. aureus. AgNPs also showed good biofilm disruption, with the highest of 88% in A. baumannii.

Conclusion: This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms.

Show MeSH
Related in: MedlinePlus