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Rapid efficient synthesis and characterization of silver, gold, and bimetallic nanoparticles from the medicinal plant Plumbago zeylanica and their application in biofilm control.

Salunke GR, Ghosh S, Santosh Kumar RJ, Khade S, Vashisth P, Kale T, Chopade S, Pruthi V, Kundu G, Bellare JR, Chopade BA - Int J Nanomedicine (2014)

Bottom Line: The synthesis of AgAuNPs, which completed within 90 minutes with 0.7 mM AgNO₃ and HAuCl₄, was found to be the fastest.Fourier-transform infrared spectroscopy confirmed bioreduction, while EDS and XRD patterns confirmed purity and the crystalline nature of the NPs, respectively.This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms.

View Article: PubMed Central - PubMed

Affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, India.

ABSTRACT

Background: Nanoparticles (NPs) have gained significance in medical fields due to their high surface-area-to-volume ratio. In this study, we synthesized NPs from a medicinally important plant - Plumbago zeylanica.

Materials and methods: Aqueous root extract of P. zeylanica (PZRE) was analyzed for the presence of flavonoids, sugars, and organic acids using high-performance thin-layer chromatography (HPTLC), gas chromatography-time of flight-mass spectrometry (GC-TOF-MS), and biochemical methods. The silver NPs (AgNPs), gold NPs (AuNPs), and bimetallic NPs (AgAuNPs) were synthesized from root extract and characterized using ultraviolet-visible spectra, X-ray diffraction (XRD), energy-dispersive spectrometry (EDS), transmission electron microscopy (TEM), and dynamic light scattering (DLS). The effects of these NPs on Acinetobacter baumannii, Staphylococcus aureus, and Escherichia coli biofilms were studied using quantitative biofilm inhibition and disruption assays, as well as using fluorescence, scanning electron microscopy, and atomic force microscopy.

Results: PZRE showed the presence of phenolics, such as plumbagin, and flavonoids, in addition to citric acid, sucrose, glucose, fructose, and starch, using HPTLC, GC-TOF-MS, and quantitative analysis. Bioreduction of silver nitrate (AgNO₃) and chloroauric acid (HAuCl₄) were confirmed at absorbances of 440 nm (AgNPs), 570 nm (AuNPs), and 540 nm (AgAuNPs), respectively. The maximum rate of synthesis at 50°C was achieved with 5 mM AgNO₃ within 4.5 hours for AgNPs; and with 0.7 mM HAuCl4 within 5 hours for AuNPs. The synthesis of AgAuNPs, which completed within 90 minutes with 0.7 mM AgNO₃ and HAuCl₄, was found to be the fastest. Fourier-transform infrared spectroscopy confirmed bioreduction, while EDS and XRD patterns confirmed purity and the crystalline nature of the NPs, respectively. TEM micrographs and DLS showed about 60 nm monodispersed Ag nanospheres, 20-30 nm Au nanospheres adhering to form Au nanotriangles, and about 90 nm hexagonal blunt-ended AgAuNPs. These NPs also showed antimicrobial and antibiofilm activity against E. coli, A. baumannii, S. aureus, and a mixed culture of A. baumannii and S. aureus. AgNPs inhibited biofilm in the range of 96%-99% and AgAuNPs from 93% to 98% in single-culture biofilms. AuNPs also showed biofilm inhibition, with the highest of 98% in S. aureus. AgNPs also showed good biofilm disruption, with the highest of 88% in A. baumannii.

Conclusion: This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms.

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Characterization of nanoparticles synthesized by PZRE using transmission electron microscopy. (A) Spherical silver nanospheres; (B) shape evolution of gold nanotriangles; (C) assembly of gold nanospheres forming a gold nanotriangle; (D) arrows indicating blunt-ended AgAu nanopolygons.Abbreviation: PZRE, Plumbago zeylanica root extract.
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f6-ijn-9-2635: Characterization of nanoparticles synthesized by PZRE using transmission electron microscopy. (A) Spherical silver nanospheres; (B) shape evolution of gold nanotriangles; (C) assembly of gold nanospheres forming a gold nanotriangle; (D) arrows indicating blunt-ended AgAu nanopolygons.Abbreviation: PZRE, Plumbago zeylanica root extract.

Mentions: The TEM micrograph showed spherically shaped AgNPs synthesized by PZRE (Figure 6A). The TEM micrographs revealed some unique features of AuNPs (Figure 6B and C). They were found to be anisotropic in nature, with spheres, triangles, and hexagons exhibiting an interesting and rare phenomenon of shape evolution. Figure 6B demonstrates an arrangement of gold nanospheres forming a gold nanotriangle. Furthermore, the shape evolution continued, with nanospheres adhering to the triangle, leading to the growth of nanotriangles with attached nanospheres (Figure 6C). In Figure 6D, the arrows indicate nanohexagons and nanospheres of AgAuNPs, but the majority of them were nanohexagons. A unique feature of AgAuNPs was their shape: blunt ended polygonal NPs. Chemically synthesized NPs were also characterized using EDS, XRD, TEM, and DLS (Figures S4–S6).


Rapid efficient synthesis and characterization of silver, gold, and bimetallic nanoparticles from the medicinal plant Plumbago zeylanica and their application in biofilm control.

Salunke GR, Ghosh S, Santosh Kumar RJ, Khade S, Vashisth P, Kale T, Chopade S, Pruthi V, Kundu G, Bellare JR, Chopade BA - Int J Nanomedicine (2014)

Characterization of nanoparticles synthesized by PZRE using transmission electron microscopy. (A) Spherical silver nanospheres; (B) shape evolution of gold nanotriangles; (C) assembly of gold nanospheres forming a gold nanotriangle; (D) arrows indicating blunt-ended AgAu nanopolygons.Abbreviation: PZRE, Plumbago zeylanica root extract.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4043712&req=5

f6-ijn-9-2635: Characterization of nanoparticles synthesized by PZRE using transmission electron microscopy. (A) Spherical silver nanospheres; (B) shape evolution of gold nanotriangles; (C) assembly of gold nanospheres forming a gold nanotriangle; (D) arrows indicating blunt-ended AgAu nanopolygons.Abbreviation: PZRE, Plumbago zeylanica root extract.
Mentions: The TEM micrograph showed spherically shaped AgNPs synthesized by PZRE (Figure 6A). The TEM micrographs revealed some unique features of AuNPs (Figure 6B and C). They were found to be anisotropic in nature, with spheres, triangles, and hexagons exhibiting an interesting and rare phenomenon of shape evolution. Figure 6B demonstrates an arrangement of gold nanospheres forming a gold nanotriangle. Furthermore, the shape evolution continued, with nanospheres adhering to the triangle, leading to the growth of nanotriangles with attached nanospheres (Figure 6C). In Figure 6D, the arrows indicate nanohexagons and nanospheres of AgAuNPs, but the majority of them were nanohexagons. A unique feature of AgAuNPs was their shape: blunt ended polygonal NPs. Chemically synthesized NPs were also characterized using EDS, XRD, TEM, and DLS (Figures S4–S6).

Bottom Line: The synthesis of AgAuNPs, which completed within 90 minutes with 0.7 mM AgNO₃ and HAuCl₄, was found to be the fastest.Fourier-transform infrared spectroscopy confirmed bioreduction, while EDS and XRD patterns confirmed purity and the crystalline nature of the NPs, respectively.This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms.

View Article: PubMed Central - PubMed

Affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, India.

ABSTRACT

Background: Nanoparticles (NPs) have gained significance in medical fields due to their high surface-area-to-volume ratio. In this study, we synthesized NPs from a medicinally important plant - Plumbago zeylanica.

Materials and methods: Aqueous root extract of P. zeylanica (PZRE) was analyzed for the presence of flavonoids, sugars, and organic acids using high-performance thin-layer chromatography (HPTLC), gas chromatography-time of flight-mass spectrometry (GC-TOF-MS), and biochemical methods. The silver NPs (AgNPs), gold NPs (AuNPs), and bimetallic NPs (AgAuNPs) were synthesized from root extract and characterized using ultraviolet-visible spectra, X-ray diffraction (XRD), energy-dispersive spectrometry (EDS), transmission electron microscopy (TEM), and dynamic light scattering (DLS). The effects of these NPs on Acinetobacter baumannii, Staphylococcus aureus, and Escherichia coli biofilms were studied using quantitative biofilm inhibition and disruption assays, as well as using fluorescence, scanning electron microscopy, and atomic force microscopy.

Results: PZRE showed the presence of phenolics, such as plumbagin, and flavonoids, in addition to citric acid, sucrose, glucose, fructose, and starch, using HPTLC, GC-TOF-MS, and quantitative analysis. Bioreduction of silver nitrate (AgNO₃) and chloroauric acid (HAuCl₄) were confirmed at absorbances of 440 nm (AgNPs), 570 nm (AuNPs), and 540 nm (AgAuNPs), respectively. The maximum rate of synthesis at 50°C was achieved with 5 mM AgNO₃ within 4.5 hours for AgNPs; and with 0.7 mM HAuCl4 within 5 hours for AuNPs. The synthesis of AgAuNPs, which completed within 90 minutes with 0.7 mM AgNO₃ and HAuCl₄, was found to be the fastest. Fourier-transform infrared spectroscopy confirmed bioreduction, while EDS and XRD patterns confirmed purity and the crystalline nature of the NPs, respectively. TEM micrographs and DLS showed about 60 nm monodispersed Ag nanospheres, 20-30 nm Au nanospheres adhering to form Au nanotriangles, and about 90 nm hexagonal blunt-ended AgAuNPs. These NPs also showed antimicrobial and antibiofilm activity against E. coli, A. baumannii, S. aureus, and a mixed culture of A. baumannii and S. aureus. AgNPs inhibited biofilm in the range of 96%-99% and AgAuNPs from 93% to 98% in single-culture biofilms. AuNPs also showed biofilm inhibition, with the highest of 98% in S. aureus. AgNPs also showed good biofilm disruption, with the highest of 88% in A. baumannii.

Conclusion: This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms.

Show MeSH
Related in: MedlinePlus