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Streptococcus pneumoniae detects and responds to foreign bacterial peptide fragments in its environment.

Hathaway LJ, Bättig P, Reber S, Rotzetter JU, Aebi S, Hauser C, Heller M, Kadioglu A, Mühlemann K - Open Biol (2014)

Bottom Line: AliB-like ORF 1 binds specifically peptide SETTFGRDFN, matching 50S ribosomal subunit protein L4 of Enterobacteriaceae, and facilitates upregulation of competence for genetic transformation.We found that AliB-like ORF 2 mediates the early phase of nasopharyngeal colonization in vivo.These findings reveal a completely new concept of pneumococcal interspecies communication which may have implications for communication between other bacterial species and for future interventional therapeutics.

View Article: PubMed Central - PubMed

Affiliation: Institute for Infectious Diseases, University of Bern, Bern, Switzerland.

ABSTRACT
Streptococcus pneumoniae is an important cause of bacterial meningitis and pneumonia but usually colonizes the human nasopharynx harmlessly. As this niche is simultaneously populated by other bacterial species, we looked for a role and pathway of communication between pneumococci and other species. This paper shows that two proteins of non-encapsulated S. pneumoniae, AliB-like ORF 1 and ORF 2, bind specifically to peptides matching other species resulting in changes in the pneumococci. AliB-like ORF 1 binds specifically peptide SETTFGRDFN, matching 50S ribosomal subunit protein L4 of Enterobacteriaceae, and facilitates upregulation of competence for genetic transformation. AliB-like ORF 2 binds specifically peptides containing sequence FPPQS, matching proteins of Prevotella species common in healthy human nasopharyngeal microbiota. We found that AliB-like ORF 2 mediates the early phase of nasopharyngeal colonization in vivo. The ability of S. pneumoniae to bind and respond to peptides of other bacterial species occupying the same host niche may play a key role in adaptation to its environment and in interspecies communication. These findings reveal a completely new concept of pneumococcal interspecies communication which may have implications for communication between other bacterial species and for future interventional therapeutics.

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Effect of incubation with E. coli on pneumococcal growth. The wild-type strain 110.58 and its mutants, as well as the control laboratory strain R6, were assessed for growth in the presence and absence of E. coli bacteria by quantifying colony forming units (cfu). Data represent the mean of three independent experiments. Error bars show s.e.m. *p < 0.05.
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RSOB130224F4: Effect of incubation with E. coli on pneumococcal growth. The wild-type strain 110.58 and its mutants, as well as the control laboratory strain R6, were assessed for growth in the presence and absence of E. coli bacteria by quantifying colony forming units (cfu). Data represent the mean of three independent experiments. Error bars show s.e.m. *p < 0.05.

Mentions: The effect of the ligands on growth of the wild-type strain and its mutants was also tested by exposing the bacteria to the free peptides, but no effect was observed (electronic supplementary material, figure S4). We also tested whether ORF 1 ligand had any effect on expression of aliB-like ORF 1 or 2 at different stages of the growth curve, but found it had none (electronic supplementary material, figure S5). We therefore went on to test whether culture with E. coli bacteria (in which the ORF 1 ligand is found within the 50S ribosomal subunit protein L4) affected growth of the pneumococci. Figure 4 shows that E. coli did indeed have a significant inhibitory effect on growth which depended on the presence of AliB-like ORF 1. In the mutants lacking AliB-like ORF 1 E. coli increased growth, an effect which was also seen, to a lesser extent, in the laboratory pneumococci strain R6 which also lacks AliB-like ORF 1.Figure 4.


Streptococcus pneumoniae detects and responds to foreign bacterial peptide fragments in its environment.

Hathaway LJ, Bättig P, Reber S, Rotzetter JU, Aebi S, Hauser C, Heller M, Kadioglu A, Mühlemann K - Open Biol (2014)

Effect of incubation with E. coli on pneumococcal growth. The wild-type strain 110.58 and its mutants, as well as the control laboratory strain R6, were assessed for growth in the presence and absence of E. coli bacteria by quantifying colony forming units (cfu). Data represent the mean of three independent experiments. Error bars show s.e.m. *p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4043112&req=5

RSOB130224F4: Effect of incubation with E. coli on pneumococcal growth. The wild-type strain 110.58 and its mutants, as well as the control laboratory strain R6, were assessed for growth in the presence and absence of E. coli bacteria by quantifying colony forming units (cfu). Data represent the mean of three independent experiments. Error bars show s.e.m. *p < 0.05.
Mentions: The effect of the ligands on growth of the wild-type strain and its mutants was also tested by exposing the bacteria to the free peptides, but no effect was observed (electronic supplementary material, figure S4). We also tested whether ORF 1 ligand had any effect on expression of aliB-like ORF 1 or 2 at different stages of the growth curve, but found it had none (electronic supplementary material, figure S5). We therefore went on to test whether culture with E. coli bacteria (in which the ORF 1 ligand is found within the 50S ribosomal subunit protein L4) affected growth of the pneumococci. Figure 4 shows that E. coli did indeed have a significant inhibitory effect on growth which depended on the presence of AliB-like ORF 1. In the mutants lacking AliB-like ORF 1 E. coli increased growth, an effect which was also seen, to a lesser extent, in the laboratory pneumococci strain R6 which also lacks AliB-like ORF 1.Figure 4.

Bottom Line: AliB-like ORF 1 binds specifically peptide SETTFGRDFN, matching 50S ribosomal subunit protein L4 of Enterobacteriaceae, and facilitates upregulation of competence for genetic transformation.We found that AliB-like ORF 2 mediates the early phase of nasopharyngeal colonization in vivo.These findings reveal a completely new concept of pneumococcal interspecies communication which may have implications for communication between other bacterial species and for future interventional therapeutics.

View Article: PubMed Central - PubMed

Affiliation: Institute for Infectious Diseases, University of Bern, Bern, Switzerland.

ABSTRACT
Streptococcus pneumoniae is an important cause of bacterial meningitis and pneumonia but usually colonizes the human nasopharynx harmlessly. As this niche is simultaneously populated by other bacterial species, we looked for a role and pathway of communication between pneumococci and other species. This paper shows that two proteins of non-encapsulated S. pneumoniae, AliB-like ORF 1 and ORF 2, bind specifically to peptides matching other species resulting in changes in the pneumococci. AliB-like ORF 1 binds specifically peptide SETTFGRDFN, matching 50S ribosomal subunit protein L4 of Enterobacteriaceae, and facilitates upregulation of competence for genetic transformation. AliB-like ORF 2 binds specifically peptides containing sequence FPPQS, matching proteins of Prevotella species common in healthy human nasopharyngeal microbiota. We found that AliB-like ORF 2 mediates the early phase of nasopharyngeal colonization in vivo. The ability of S. pneumoniae to bind and respond to peptides of other bacterial species occupying the same host niche may play a key role in adaptation to its environment and in interspecies communication. These findings reveal a completely new concept of pneumococcal interspecies communication which may have implications for communication between other bacterial species and for future interventional therapeutics.

Show MeSH
Related in: MedlinePlus