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B7-H3 overexpression in pancreatic cancer promotes tumor progression.

Zhao X, Li DC, Zhu XG, Gan WJ, Li Z, Xiong F, Zhang ZX, Zhang GB, Zhang XG, Zhao H - Int. J. Mol. Med. (2012)

Bottom Line: In the present study, we compared B7-H3 expression in normal pancreas and pancreatic cancer tissue specimens, and determined the effects of low B7-H3 expression on the human pancreatic cancer cell line Patu8988 using lentivirus-mediated RNA interference.This demonstrated that inhibition of B7-H3 expression reduced pancreatic cancer metastasis in vivo.In conclusion, B7-H3 is aberrantly expressed in pancreatic cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, P.R. China.

ABSTRACT
B7-H3, a member of the B7-family molecules, plays an important role in adaptive immune responses. In addition, B7-H3 is also expressed in several types of human cancers and is correlated with the poor outcome of cancer patients. However, its exact role in cancer is not known. In the present study, we compared B7-H3 expression in normal pancreas and pancreatic cancer tissue specimens, and determined the effects of low B7-H3 expression on the human pancreatic cancer cell line Patu8988 using lentivirus-mediated RNA interference. B7-H3 expression in pancreatic specimens was determined by enzyme-linked immunosorbent assay (ELISA). A Patu8988 cell line with low B7-H3 expression was established by lentivirus-mediated RNA interference to investigate the effect of B7-H3 on cell proliferation, migration and invasion in vitro. By establishing subcutaneous transplantation tumor and orthotopic transplantation pancreatic cancer mouse models, the effect of B7-H3 on cell proliferation, migration and invasion was studied in vivo. B7-H3 in tissue samples was significantly higher in the pancreatic cancer group than in the normal pancreas group (mean ± SD, 193.6±9.352 vs. 87.74±7.433 ng/g; P<0.0001). B7-H3 knockdown by RNA interference decreased cell migration and Transwell invasion up to 50% in vitro. No apparent impact was observed on cell proliferation in vitro. In the subcutaneous transplantation tumor mouse model, the tumor growth rate was reduced by the knockdown of B7-H3. In the orthotopic transplantation pancreatic cancer mouse model, the effect of inhibiting metastasis by knocking down B7-H3 was assessed in terms of the average postmortem abdominal visceral metastatic tumor weight. This demonstrated that inhibition of B7-H3 expression reduced pancreatic cancer metastasis in vivo. In conclusion, B7-H3 is aberrantly expressed in pancreatic cancer. In addition to modulating tumor immunity, B7-H3 may have a novel role in regulating pancreatic tumor progression.

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Immunohistochemical staining for B7-H3 in subcutaneous xenotransplantation tumors.Xenografts from the LV-NC and the control group showed distinct membranous andcytoplasmic immunoreactivity for B7-H3, while xenografts from the LV-B7-H3 group showedweak B7-H3 expression. (Magnification, ×400).
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f9-ijmm-31-02-0283: Immunohistochemical staining for B7-H3 in subcutaneous xenotransplantation tumors.Xenografts from the LV-NC and the control group showed distinct membranous andcytoplasmic immunoreactivity for B7-H3, while xenografts from the LV-B7-H3 group showedweak B7-H3 expression. (Magnification, ×400).

Mentions: The knockdown of B7-H3 in the xenografts was confirmed by immunohistochemical staining.While the level of B7-H3 expression retaimed low in the LV-B7-H3 group tumors, the LV-NCand control tumors showed strong staining (Fig. 9).


B7-H3 overexpression in pancreatic cancer promotes tumor progression.

Zhao X, Li DC, Zhu XG, Gan WJ, Li Z, Xiong F, Zhang ZX, Zhang GB, Zhang XG, Zhao H - Int. J. Mol. Med. (2012)

Immunohistochemical staining for B7-H3 in subcutaneous xenotransplantation tumors.Xenografts from the LV-NC and the control group showed distinct membranous andcytoplasmic immunoreactivity for B7-H3, while xenografts from the LV-B7-H3 group showedweak B7-H3 expression. (Magnification, ×400).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4042878&req=5

f9-ijmm-31-02-0283: Immunohistochemical staining for B7-H3 in subcutaneous xenotransplantation tumors.Xenografts from the LV-NC and the control group showed distinct membranous andcytoplasmic immunoreactivity for B7-H3, while xenografts from the LV-B7-H3 group showedweak B7-H3 expression. (Magnification, ×400).
Mentions: The knockdown of B7-H3 in the xenografts was confirmed by immunohistochemical staining.While the level of B7-H3 expression retaimed low in the LV-B7-H3 group tumors, the LV-NCand control tumors showed strong staining (Fig. 9).

Bottom Line: In the present study, we compared B7-H3 expression in normal pancreas and pancreatic cancer tissue specimens, and determined the effects of low B7-H3 expression on the human pancreatic cancer cell line Patu8988 using lentivirus-mediated RNA interference.This demonstrated that inhibition of B7-H3 expression reduced pancreatic cancer metastasis in vivo.In conclusion, B7-H3 is aberrantly expressed in pancreatic cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, P.R. China.

ABSTRACT
B7-H3, a member of the B7-family molecules, plays an important role in adaptive immune responses. In addition, B7-H3 is also expressed in several types of human cancers and is correlated with the poor outcome of cancer patients. However, its exact role in cancer is not known. In the present study, we compared B7-H3 expression in normal pancreas and pancreatic cancer tissue specimens, and determined the effects of low B7-H3 expression on the human pancreatic cancer cell line Patu8988 using lentivirus-mediated RNA interference. B7-H3 expression in pancreatic specimens was determined by enzyme-linked immunosorbent assay (ELISA). A Patu8988 cell line with low B7-H3 expression was established by lentivirus-mediated RNA interference to investigate the effect of B7-H3 on cell proliferation, migration and invasion in vitro. By establishing subcutaneous transplantation tumor and orthotopic transplantation pancreatic cancer mouse models, the effect of B7-H3 on cell proliferation, migration and invasion was studied in vivo. B7-H3 in tissue samples was significantly higher in the pancreatic cancer group than in the normal pancreas group (mean ± SD, 193.6±9.352 vs. 87.74±7.433 ng/g; P<0.0001). B7-H3 knockdown by RNA interference decreased cell migration and Transwell invasion up to 50% in vitro. No apparent impact was observed on cell proliferation in vitro. In the subcutaneous transplantation tumor mouse model, the tumor growth rate was reduced by the knockdown of B7-H3. In the orthotopic transplantation pancreatic cancer mouse model, the effect of inhibiting metastasis by knocking down B7-H3 was assessed in terms of the average postmortem abdominal visceral metastatic tumor weight. This demonstrated that inhibition of B7-H3 expression reduced pancreatic cancer metastasis in vivo. In conclusion, B7-H3 is aberrantly expressed in pancreatic cancer. In addition to modulating tumor immunity, B7-H3 may have a novel role in regulating pancreatic tumor progression.

Show MeSH
Related in: MedlinePlus