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B7-H3 overexpression in pancreatic cancer promotes tumor progression.

Zhao X, Li DC, Zhu XG, Gan WJ, Li Z, Xiong F, Zhang ZX, Zhang GB, Zhang XG, Zhao H - Int. J. Mol. Med. (2012)

Bottom Line: In the present study, we compared B7-H3 expression in normal pancreas and pancreatic cancer tissue specimens, and determined the effects of low B7-H3 expression on the human pancreatic cancer cell line Patu8988 using lentivirus-mediated RNA interference.This demonstrated that inhibition of B7-H3 expression reduced pancreatic cancer metastasis in vivo.In conclusion, B7-H3 is aberrantly expressed in pancreatic cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, P.R. China.

ABSTRACT
B7-H3, a member of the B7-family molecules, plays an important role in adaptive immune responses. In addition, B7-H3 is also expressed in several types of human cancers and is correlated with the poor outcome of cancer patients. However, its exact role in cancer is not known. In the present study, we compared B7-H3 expression in normal pancreas and pancreatic cancer tissue specimens, and determined the effects of low B7-H3 expression on the human pancreatic cancer cell line Patu8988 using lentivirus-mediated RNA interference. B7-H3 expression in pancreatic specimens was determined by enzyme-linked immunosorbent assay (ELISA). A Patu8988 cell line with low B7-H3 expression was established by lentivirus-mediated RNA interference to investigate the effect of B7-H3 on cell proliferation, migration and invasion in vitro. By establishing subcutaneous transplantation tumor and orthotopic transplantation pancreatic cancer mouse models, the effect of B7-H3 on cell proliferation, migration and invasion was studied in vivo. B7-H3 in tissue samples was significantly higher in the pancreatic cancer group than in the normal pancreas group (mean ± SD, 193.6±9.352 vs. 87.74±7.433 ng/g; P<0.0001). B7-H3 knockdown by RNA interference decreased cell migration and Transwell invasion up to 50% in vitro. No apparent impact was observed on cell proliferation in vitro. In the subcutaneous transplantation tumor mouse model, the tumor growth rate was reduced by the knockdown of B7-H3. In the orthotopic transplantation pancreatic cancer mouse model, the effect of inhibiting metastasis by knocking down B7-H3 was assessed in terms of the average postmortem abdominal visceral metastatic tumor weight. This demonstrated that inhibition of B7-H3 expression reduced pancreatic cancer metastasis in vivo. In conclusion, B7-H3 is aberrantly expressed in pancreatic cancer. In addition to modulating tumor immunity, B7-H3 may have a novel role in regulating pancreatic tumor progression.

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B7-H3 was significantly higher in pancreatic cancer than in normal pancreas tissuesamples. (*P<0.0001, pancreatic cancer vs. normalpancreas).
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f1-ijmm-31-02-0283: B7-H3 was significantly higher in pancreatic cancer than in normal pancreas tissuesamples. (*P<0.0001, pancreatic cancer vs. normalpancreas).

Mentions: B7-H3 levels in the pancreatic cancer group were significantly higher than that in thenormal pancreas group (mean 193.6±9.352 vs. 87.74±7.433 ng/g,P<0.0001) (Fig. 1).Immunohistochemical staining revealed significantly overexpressed B7-H3 in tumor tissue(Chi-square test 15.341; P<0.001). Positive staining for B7-H3 expression wasdetected in more than 50% of cells in 17 of the 26 pancreatic cancer specimenswhile no positive cells were detected in normal pancreas specimens (Fig. 2).


B7-H3 overexpression in pancreatic cancer promotes tumor progression.

Zhao X, Li DC, Zhu XG, Gan WJ, Li Z, Xiong F, Zhang ZX, Zhang GB, Zhang XG, Zhao H - Int. J. Mol. Med. (2012)

B7-H3 was significantly higher in pancreatic cancer than in normal pancreas tissuesamples. (*P<0.0001, pancreatic cancer vs. normalpancreas).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4042878&req=5

f1-ijmm-31-02-0283: B7-H3 was significantly higher in pancreatic cancer than in normal pancreas tissuesamples. (*P<0.0001, pancreatic cancer vs. normalpancreas).
Mentions: B7-H3 levels in the pancreatic cancer group were significantly higher than that in thenormal pancreas group (mean 193.6±9.352 vs. 87.74±7.433 ng/g,P<0.0001) (Fig. 1).Immunohistochemical staining revealed significantly overexpressed B7-H3 in tumor tissue(Chi-square test 15.341; P<0.001). Positive staining for B7-H3 expression wasdetected in more than 50% of cells in 17 of the 26 pancreatic cancer specimenswhile no positive cells were detected in normal pancreas specimens (Fig. 2).

Bottom Line: In the present study, we compared B7-H3 expression in normal pancreas and pancreatic cancer tissue specimens, and determined the effects of low B7-H3 expression on the human pancreatic cancer cell line Patu8988 using lentivirus-mediated RNA interference.This demonstrated that inhibition of B7-H3 expression reduced pancreatic cancer metastasis in vivo.In conclusion, B7-H3 is aberrantly expressed in pancreatic cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, P.R. China.

ABSTRACT
B7-H3, a member of the B7-family molecules, plays an important role in adaptive immune responses. In addition, B7-H3 is also expressed in several types of human cancers and is correlated with the poor outcome of cancer patients. However, its exact role in cancer is not known. In the present study, we compared B7-H3 expression in normal pancreas and pancreatic cancer tissue specimens, and determined the effects of low B7-H3 expression on the human pancreatic cancer cell line Patu8988 using lentivirus-mediated RNA interference. B7-H3 expression in pancreatic specimens was determined by enzyme-linked immunosorbent assay (ELISA). A Patu8988 cell line with low B7-H3 expression was established by lentivirus-mediated RNA interference to investigate the effect of B7-H3 on cell proliferation, migration and invasion in vitro. By establishing subcutaneous transplantation tumor and orthotopic transplantation pancreatic cancer mouse models, the effect of B7-H3 on cell proliferation, migration and invasion was studied in vivo. B7-H3 in tissue samples was significantly higher in the pancreatic cancer group than in the normal pancreas group (mean ± SD, 193.6±9.352 vs. 87.74±7.433 ng/g; P<0.0001). B7-H3 knockdown by RNA interference decreased cell migration and Transwell invasion up to 50% in vitro. No apparent impact was observed on cell proliferation in vitro. In the subcutaneous transplantation tumor mouse model, the tumor growth rate was reduced by the knockdown of B7-H3. In the orthotopic transplantation pancreatic cancer mouse model, the effect of inhibiting metastasis by knocking down B7-H3 was assessed in terms of the average postmortem abdominal visceral metastatic tumor weight. This demonstrated that inhibition of B7-H3 expression reduced pancreatic cancer metastasis in vivo. In conclusion, B7-H3 is aberrantly expressed in pancreatic cancer. In addition to modulating tumor immunity, B7-H3 may have a novel role in regulating pancreatic tumor progression.

Show MeSH
Related in: MedlinePlus