Limits...
Calcaridorylaimus castaneae sp. n. (Nematoda, Dorylaimidae) from Bulgaria with an identification key to the species of the genus.

Nedelchev S, Elshishka M, Lazarova S, Radoslavov G, Hristov P, Peneva V - Zookeys (2014)

Bottom Line: Phylogenetic analyses were performed on 18S and D2-D3 expansion domains of 28S rRNA genes by Neighbor-Joining, Maximum Likelihood and Bayesian Inference methods.The phylograms inferred from 18S sequences showed closest relationships of the new species with some species belonging to the genus Mesodorylaimus.However, insufficient molecular data for members of both genera do not allow the phylogenetic relationships of Calcaridorylaimus and the new species described herein to be elucidated.

View Article: PubMed Central - HTML - PubMed

Affiliation: Faculty of Biology, University of Sofia, Bd. "Dragan Tzankov" 8, 1421 Sofia, Bulgaria.

ABSTRACT
An unknown species belonging to the genusCalcaridorylaimus Andrássy, 1986 was collected from the litter of broadleaf forests dominated by Castanea sativa Mill. and mixed with Quercus daleshampii Ten. and Fagus sylvatica L. on Belasitsa Mountain, south-western Bulgaria. Calcaridorylaimus castaneae sp. n. is characterised by its long body (1.4-2.1 mm), lip region practically not offset, vulva transverse, short odontostyle (14.5-16 μm) and tail (75.5-110.5 μm, c=14.7-23.6; c'=2.9-4.4) in females and 38-46 μm long spicules with small spur before their distant end in males. It is most similar to C. andrassyi Ahmad & Shaheen, 2004, but differs in having transverse vs pore-like vulva and shorter spicules (38-46 μm vs 52-57 μm). An identification key to the species of the genus Calcaridorylaimus is proposed. Phylogenetic analyses were performed on 18S and D2-D3 expansion domains of 28S rRNA genes by Neighbor-Joining, Maximum Likelihood and Bayesian Inference methods. The phylograms inferred from 18S sequences showed closest relationships of the new species with some species belonging to the genus Mesodorylaimus. However, insufficient molecular data for members of both genera do not allow the phylogenetic relationships of Calcaridorylaimus and the new species described herein to be elucidated.

No MeSH data available.


Phylogenetic relationships of Calcaridorylaimus castaneae sp. n. and its closest species for 18S rRNA gene. Bayesian Inference strict consensus tree acquired under GTR+G model. Posterior probabilities higher than 0.8 are presented.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4042707&req=5

Figure 7: Phylogenetic relationships of Calcaridorylaimus castaneae sp. n. and its closest species for 18S rRNA gene. Bayesian Inference strict consensus tree acquired under GTR+G model. Posterior probabilities higher than 0.8 are presented.

Mentions: The phylogenetic analyses based on 18S rDNA and D2-D3 of 28S rDNA sequences from various dorylaimid species with the highest matches of the BLAST search (up to 97% and 85%, respectively) were aligned along with our sequence. The phylograms obtained by NJ, ML and BI methods showed similar topology and differed only in the positions of poorly supported clades. The BI trees (Figs 7 and 8) with posterior probabilities higher than 0.8 and NJ-ML trees with bootstrap values above 70% are presented (Figs 9 and 10). The new species has clustered in a well-supported group with several Mesodorylaimus spp. (Mesodorylaimus bastiani (Bütschli, 1873), Mesodorylaimus japonicus (Cobb in Thorne and Swanger, 1936) and Mesodorylaimus cf. nigritulus (Schneider, 1937)) and two unidentified nematodes from environmental samples in the 18S rDNA phylogenetic reconstructions (Figs 7 and 9); and to more distantly related species from various dorylaimid genera and families (Prodorylaimus, Labronema, Nevadanema, Paractinolaimus) in the partial D2-D3 LSU reconstructions (Figs 8 and 10).


Calcaridorylaimus castaneae sp. n. (Nematoda, Dorylaimidae) from Bulgaria with an identification key to the species of the genus.

Nedelchev S, Elshishka M, Lazarova S, Radoslavov G, Hristov P, Peneva V - Zookeys (2014)

Phylogenetic relationships of Calcaridorylaimus castaneae sp. n. and its closest species for 18S rRNA gene. Bayesian Inference strict consensus tree acquired under GTR+G model. Posterior probabilities higher than 0.8 are presented.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4042707&req=5

Figure 7: Phylogenetic relationships of Calcaridorylaimus castaneae sp. n. and its closest species for 18S rRNA gene. Bayesian Inference strict consensus tree acquired under GTR+G model. Posterior probabilities higher than 0.8 are presented.
Mentions: The phylogenetic analyses based on 18S rDNA and D2-D3 of 28S rDNA sequences from various dorylaimid species with the highest matches of the BLAST search (up to 97% and 85%, respectively) were aligned along with our sequence. The phylograms obtained by NJ, ML and BI methods showed similar topology and differed only in the positions of poorly supported clades. The BI trees (Figs 7 and 8) with posterior probabilities higher than 0.8 and NJ-ML trees with bootstrap values above 70% are presented (Figs 9 and 10). The new species has clustered in a well-supported group with several Mesodorylaimus spp. (Mesodorylaimus bastiani (Bütschli, 1873), Mesodorylaimus japonicus (Cobb in Thorne and Swanger, 1936) and Mesodorylaimus cf. nigritulus (Schneider, 1937)) and two unidentified nematodes from environmental samples in the 18S rDNA phylogenetic reconstructions (Figs 7 and 9); and to more distantly related species from various dorylaimid genera and families (Prodorylaimus, Labronema, Nevadanema, Paractinolaimus) in the partial D2-D3 LSU reconstructions (Figs 8 and 10).

Bottom Line: Phylogenetic analyses were performed on 18S and D2-D3 expansion domains of 28S rRNA genes by Neighbor-Joining, Maximum Likelihood and Bayesian Inference methods.The phylograms inferred from 18S sequences showed closest relationships of the new species with some species belonging to the genus Mesodorylaimus.However, insufficient molecular data for members of both genera do not allow the phylogenetic relationships of Calcaridorylaimus and the new species described herein to be elucidated.

View Article: PubMed Central - HTML - PubMed

Affiliation: Faculty of Biology, University of Sofia, Bd. "Dragan Tzankov" 8, 1421 Sofia, Bulgaria.

ABSTRACT
An unknown species belonging to the genusCalcaridorylaimus Andrássy, 1986 was collected from the litter of broadleaf forests dominated by Castanea sativa Mill. and mixed with Quercus daleshampii Ten. and Fagus sylvatica L. on Belasitsa Mountain, south-western Bulgaria. Calcaridorylaimus castaneae sp. n. is characterised by its long body (1.4-2.1 mm), lip region practically not offset, vulva transverse, short odontostyle (14.5-16 μm) and tail (75.5-110.5 μm, c=14.7-23.6; c'=2.9-4.4) in females and 38-46 μm long spicules with small spur before their distant end in males. It is most similar to C. andrassyi Ahmad & Shaheen, 2004, but differs in having transverse vs pore-like vulva and shorter spicules (38-46 μm vs 52-57 μm). An identification key to the species of the genus Calcaridorylaimus is proposed. Phylogenetic analyses were performed on 18S and D2-D3 expansion domains of 28S rRNA genes by Neighbor-Joining, Maximum Likelihood and Bayesian Inference methods. The phylograms inferred from 18S sequences showed closest relationships of the new species with some species belonging to the genus Mesodorylaimus. However, insufficient molecular data for members of both genera do not allow the phylogenetic relationships of Calcaridorylaimus and the new species described herein to be elucidated.

No MeSH data available.