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Phylogenetic relationships of Malaysia's long-tailed macaques, Macaca fascicularis, based on cytochrome b sequences.

Abdul-Latiff MA, Ruslin F, Fui VV, Abu MH, Rovie-Ryan JJ, Abdul-Patah P, Lakim M, Roos C, Yaakop S, Md-Zain BM - Zookeys (2014)

Bottom Line: The results confirm the previous morphological assignment of 2 subspecies, M. f. fascicularis and M. f. argentimembris, in the Malay Peninsula.These populations should be treated as separate genetic entities in order to conserve the genetic diversity of Malaysia's M. fascicularis.These findings are crucial in aiding the conservation management and translocation process of M. fascicularis populations in Malaysia.

View Article: PubMed Central - PubMed

Affiliation: School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia.

ABSTRACT
Phylogenetic relationships among Malaysia's long-tailed macaques have yet to be established, despite abundant genetic studies of the species worldwide. The aims of this study are to examine the phylogenetic relationships of Macaca fascicularis in Malaysia and to test its classification as a morphological subspecies. A total of 25 genetic samples of M. fascicularis yielding 383 bp of Cytochrome b (Cyt b) sequences were used in phylogenetic analysis along with one sample each of M. nemestrina and M. arctoides used as outgroups. Sequence character analysis reveals that Cyt b locus is a highly conserved region with only 23% parsimony informative character detected among ingroups. Further analysis indicates a clear separation between populations originating from different regions; the Malay Peninsula versus Borneo Insular, the East Coast versus West Coast of the Malay Peninsula, and the island versus mainland Malay Peninsula populations. Phylogenetic trees (NJ, MP and Bayesian) portray a consistent clustering paradigm as Borneo's population was distinguished from Peninsula's population (99% and 100% bootstrap value in NJ and MP respectively and 1.00 posterior probability in Bayesian trees). The East coast population was separated from other Peninsula populations (64% in NJ, 66% in MP and 0.53 posterior probability in Bayesian). West coast populations were divided into 2 clades: the North-South (47%/54% in NJ, 26/26% in MP and 1.00/0.80 posterior probability in Bayesian) and Island-Mainland (93% in NJ, 90% in MP and 1.00 posterior probability in Bayesian). The results confirm the previous morphological assignment of 2 subspecies, M. f. fascicularis and M. f. argentimembris, in the Malay Peninsula. These populations should be treated as separate genetic entities in order to conserve the genetic diversity of Malaysia's M. fascicularis. These findings are crucial in aiding the conservation management and translocation process of M. fascicularis populations in Malaysia.

No MeSH data available.


Related in: MedlinePlus

Sampling location of Macaca fascicularis throughout Peninsula Malaysia and Borneo.
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Figure 2: Sampling location of Macaca fascicularis throughout Peninsula Malaysia and Borneo.

Mentions: Altogether, 27 genetic samples (Table 1 and Figure 2) were used in this research. These samples were provided by the Department of Wildlife and National Parks (PERHILITAN) and Sabah Parks. The samples derive mainly from feces collected in the original habitats of Macaca fascicularis. In addition, blood and tissue samples collected from a roadkill specimen of Macaca fascicularis were also used in this study. Mitochondrial DNA (mtDNA) was extracted from each genetic sample using QIAGEN DNeasy Blood and Tissue Kit, following the manufacturer’s protocol. A mtDNA genome from FTA (fast technology for analysis of nucleic acids) sample was extracted using the WHATMAN® GenSolve Recovery Kit, also following the manufacturer’s protocol. DNA was extracted from 0.5 g – 1.0 g of fecal sample using innuPREP Stool DNA kit (Analytik Jena) following the manufacturer’s protocol.


Phylogenetic relationships of Malaysia's long-tailed macaques, Macaca fascicularis, based on cytochrome b sequences.

Abdul-Latiff MA, Ruslin F, Fui VV, Abu MH, Rovie-Ryan JJ, Abdul-Patah P, Lakim M, Roos C, Yaakop S, Md-Zain BM - Zookeys (2014)

Sampling location of Macaca fascicularis throughout Peninsula Malaysia and Borneo.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4042704&req=5

Figure 2: Sampling location of Macaca fascicularis throughout Peninsula Malaysia and Borneo.
Mentions: Altogether, 27 genetic samples (Table 1 and Figure 2) were used in this research. These samples were provided by the Department of Wildlife and National Parks (PERHILITAN) and Sabah Parks. The samples derive mainly from feces collected in the original habitats of Macaca fascicularis. In addition, blood and tissue samples collected from a roadkill specimen of Macaca fascicularis were also used in this study. Mitochondrial DNA (mtDNA) was extracted from each genetic sample using QIAGEN DNeasy Blood and Tissue Kit, following the manufacturer’s protocol. A mtDNA genome from FTA (fast technology for analysis of nucleic acids) sample was extracted using the WHATMAN® GenSolve Recovery Kit, also following the manufacturer’s protocol. DNA was extracted from 0.5 g – 1.0 g of fecal sample using innuPREP Stool DNA kit (Analytik Jena) following the manufacturer’s protocol.

Bottom Line: The results confirm the previous morphological assignment of 2 subspecies, M. f. fascicularis and M. f. argentimembris, in the Malay Peninsula.These populations should be treated as separate genetic entities in order to conserve the genetic diversity of Malaysia's M. fascicularis.These findings are crucial in aiding the conservation management and translocation process of M. fascicularis populations in Malaysia.

View Article: PubMed Central - PubMed

Affiliation: School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia.

ABSTRACT
Phylogenetic relationships among Malaysia's long-tailed macaques have yet to be established, despite abundant genetic studies of the species worldwide. The aims of this study are to examine the phylogenetic relationships of Macaca fascicularis in Malaysia and to test its classification as a morphological subspecies. A total of 25 genetic samples of M. fascicularis yielding 383 bp of Cytochrome b (Cyt b) sequences were used in phylogenetic analysis along with one sample each of M. nemestrina and M. arctoides used as outgroups. Sequence character analysis reveals that Cyt b locus is a highly conserved region with only 23% parsimony informative character detected among ingroups. Further analysis indicates a clear separation between populations originating from different regions; the Malay Peninsula versus Borneo Insular, the East Coast versus West Coast of the Malay Peninsula, and the island versus mainland Malay Peninsula populations. Phylogenetic trees (NJ, MP and Bayesian) portray a consistent clustering paradigm as Borneo's population was distinguished from Peninsula's population (99% and 100% bootstrap value in NJ and MP respectively and 1.00 posterior probability in Bayesian trees). The East coast population was separated from other Peninsula populations (64% in NJ, 66% in MP and 0.53 posterior probability in Bayesian). West coast populations were divided into 2 clades: the North-South (47%/54% in NJ, 26/26% in MP and 1.00/0.80 posterior probability in Bayesian) and Island-Mainland (93% in NJ, 90% in MP and 1.00 posterior probability in Bayesian). The results confirm the previous morphological assignment of 2 subspecies, M. f. fascicularis and M. f. argentimembris, in the Malay Peninsula. These populations should be treated as separate genetic entities in order to conserve the genetic diversity of Malaysia's M. fascicularis. These findings are crucial in aiding the conservation management and translocation process of M. fascicularis populations in Malaysia.

No MeSH data available.


Related in: MedlinePlus