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Blood flow restricted resistance training attenuates myostatin gene expression in a patient with inclusion body myositis.

Santos AR, Neves MT, Gualano B, Laurentino GC, Lancha AH, Ugrinowitsch C, Lima FR, Aoki MS - Biol Sport (2014)

Bottom Line: After 12 weeks of training, a decrease (25%) in MSTN mRNA level was observed, whereas follistatin and follistatin-like 3 gene expression increased by 40% and 70%, respectively.FOXO-3 and MURF-2 gene expression increased by 40% and 20%, respectively.No change was observed in activin II B receptor or MyoD gene expression.

View Article: PubMed Central - PubMed

Affiliation: School of Physical Education and Sport, University of São Paulo, Brazil ; Both authors contributed equally to this paper.

ABSTRACT

Unlabelled: Inclusion body myositis is a rare idiopathic inflammatory myopathy that produces extreme muscle weakness. Blood flow restricted resistance training has been shown to improve muscle strength and muscle hypertrophy in inclusion body myositis.

Objective: The aim of this study was to evaluate the effects of a resistance training programme on the expression of genes related to myostatin (MSTN) signalling in one inclusion body myositis patient.

Methods: A 65-year-old man with inclusion body myositis underwent blood flow restricted resistance training for 12 weeks. The gene expression of MSTN, follistatin, follistatin-like 3, activin II B receptor, SMAD-7, MyoD, FOXO-3, and MURF-2 was quantified.

Results: After 12 weeks of training, a decrease (25%) in MSTN mRNA level was observed, whereas follistatin and follistatin-like 3 gene expression increased by 40% and 70%, respectively. SMAD-7 mRNA level was augmented (20%). FOXO-3 and MURF-2 gene expression increased by 40% and 20%, respectively. No change was observed in activin II B receptor or MyoD gene expression.

Conclusions: Blood flow restricted resistance training attenuated MSTN gene expression and also increased expression of myostatin endogenous inhibitors. Blood flow restricted resistance training evoked changes in the expression of genes related to MSTN signalling pathway that could in part explain the muscle hypertrophy previously observed in a patient with inclusion body myositis.

No MeSH data available.


Related in: MedlinePlus

THE EFFECTS OF 12-WEEK BFRRT ON MRNA EXPRESSION LEVELS OF MSTN, FLST, FL-3, AND ACTIIB IN A PATIENT WITH IBM.Note: The post-training values are reported as fold-change relative to baseline expression at the start of the study.
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Figure 0001: THE EFFECTS OF 12-WEEK BFRRT ON MRNA EXPRESSION LEVELS OF MSTN, FLST, FL-3, AND ACTIIB IN A PATIENT WITH IBM.Note: The post-training values are reported as fold-change relative to baseline expression at the start of the study.

Mentions: After 12 weeks of training, a moderate decrease in MSTN mRNA expression (25%) was observed, whereas FLST and FL-3 mRNAs expression increased (FLST: 40% and FL-3: 70%) (Figure 1). SMAD-7 mRNA level was slightly enhanced (20%) (Figure 2). FOXO-3 and MURF-2 gene expression increased by 40% and 20%, respectively (Figure 2). No change was observed in ActIIB and MyoD gene expression (Figures 1 and 2 respectively).


Blood flow restricted resistance training attenuates myostatin gene expression in a patient with inclusion body myositis.

Santos AR, Neves MT, Gualano B, Laurentino GC, Lancha AH, Ugrinowitsch C, Lima FR, Aoki MS - Biol Sport (2014)

THE EFFECTS OF 12-WEEK BFRRT ON MRNA EXPRESSION LEVELS OF MSTN, FLST, FL-3, AND ACTIIB IN A PATIENT WITH IBM.Note: The post-training values are reported as fold-change relative to baseline expression at the start of the study.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4042658&req=5

Figure 0001: THE EFFECTS OF 12-WEEK BFRRT ON MRNA EXPRESSION LEVELS OF MSTN, FLST, FL-3, AND ACTIIB IN A PATIENT WITH IBM.Note: The post-training values are reported as fold-change relative to baseline expression at the start of the study.
Mentions: After 12 weeks of training, a moderate decrease in MSTN mRNA expression (25%) was observed, whereas FLST and FL-3 mRNAs expression increased (FLST: 40% and FL-3: 70%) (Figure 1). SMAD-7 mRNA level was slightly enhanced (20%) (Figure 2). FOXO-3 and MURF-2 gene expression increased by 40% and 20%, respectively (Figure 2). No change was observed in ActIIB and MyoD gene expression (Figures 1 and 2 respectively).

Bottom Line: After 12 weeks of training, a decrease (25%) in MSTN mRNA level was observed, whereas follistatin and follistatin-like 3 gene expression increased by 40% and 70%, respectively.FOXO-3 and MURF-2 gene expression increased by 40% and 20%, respectively.No change was observed in activin II B receptor or MyoD gene expression.

View Article: PubMed Central - PubMed

Affiliation: School of Physical Education and Sport, University of São Paulo, Brazil ; Both authors contributed equally to this paper.

ABSTRACT

Unlabelled: Inclusion body myositis is a rare idiopathic inflammatory myopathy that produces extreme muscle weakness. Blood flow restricted resistance training has been shown to improve muscle strength and muscle hypertrophy in inclusion body myositis.

Objective: The aim of this study was to evaluate the effects of a resistance training programme on the expression of genes related to myostatin (MSTN) signalling in one inclusion body myositis patient.

Methods: A 65-year-old man with inclusion body myositis underwent blood flow restricted resistance training for 12 weeks. The gene expression of MSTN, follistatin, follistatin-like 3, activin II B receptor, SMAD-7, MyoD, FOXO-3, and MURF-2 was quantified.

Results: After 12 weeks of training, a decrease (25%) in MSTN mRNA level was observed, whereas follistatin and follistatin-like 3 gene expression increased by 40% and 70%, respectively. SMAD-7 mRNA level was augmented (20%). FOXO-3 and MURF-2 gene expression increased by 40% and 20%, respectively. No change was observed in activin II B receptor or MyoD gene expression.

Conclusions: Blood flow restricted resistance training attenuated MSTN gene expression and also increased expression of myostatin endogenous inhibitors. Blood flow restricted resistance training evoked changes in the expression of genes related to MSTN signalling pathway that could in part explain the muscle hypertrophy previously observed in a patient with inclusion body myositis.

No MeSH data available.


Related in: MedlinePlus