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Vagus nerve controls resolution and pro-resolving mediators of inflammation.

Mirakaj V, Dalli J, Granja T, Rosenberger P, Serhan CN - J. Exp. Med. (2014)

Bottom Line: In netrin-1(+/-) mice, resolvin D1 (RvD1) was less effective in reducing neutrophil influx promoting resolution of peritonitis compared with Ntn1(+/+).Human monocytes incubated with netrin-1 produced proresolving mediators, including resolvins and lipoxins.Netrin-1 and RvD1 displayed bidirectional activation in that they stimulated each other's expression and enhanced efferocytosis.

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Affiliation: Center for Experimental Therapeutics and Reperfusion Injury, Harvard Institutes of Medicine, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115 Clinic of Anesthesiology and Intensive Care Medicine, University Hospital Tübingen, Eberhard-Karls University, 72076 Tübingen, Germany.

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Vagotomy reduces netrin-1 expression. Animals were subjected to unilateral vagotomy or a comparable sham surgical procedure and netrin-1 and neurofilament expression in lungs was assessed by immunofluorescence. (A) Colocalization (green) of netrin-1 with the neuronal marker neurofilament (red) and yellow in the merged image. Bars, 100 µm. Results are representative of two separate experiments (n = 3 mice per group). (B) Leukocytes isolated from human peripheral blood were incubated with an antibody against netrin-1, CD15, CD14, CD68, CD3, CD19, and the expression of indicated marker was measured by flow cytometry. Results are representative of two separate experiments (from n = 3 healthy donors).
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fig5: Vagotomy reduces netrin-1 expression. Animals were subjected to unilateral vagotomy or a comparable sham surgical procedure and netrin-1 and neurofilament expression in lungs was assessed by immunofluorescence. (A) Colocalization (green) of netrin-1 with the neuronal marker neurofilament (red) and yellow in the merged image. Bars, 100 µm. Results are representative of two separate experiments (n = 3 mice per group). (B) Leukocytes isolated from human peripheral blood were incubated with an antibody against netrin-1, CD15, CD14, CD68, CD3, CD19, and the expression of indicated marker was measured by flow cytometry. Results are representative of two separate experiments (from n = 3 healthy donors).

Mentions: As we considered that the netrin-1 expression is likely regulated neuronally, we performed immunochemistry staining of lungs from vagotomized or sham-operated mice to determine netrin-1 expression. We identified reduced expression of netrin-1 within lungs from vagotomized mice, especially on neurofilament structures (Fig. 5 A).


Vagus nerve controls resolution and pro-resolving mediators of inflammation.

Mirakaj V, Dalli J, Granja T, Rosenberger P, Serhan CN - J. Exp. Med. (2014)

Vagotomy reduces netrin-1 expression. Animals were subjected to unilateral vagotomy or a comparable sham surgical procedure and netrin-1 and neurofilament expression in lungs was assessed by immunofluorescence. (A) Colocalization (green) of netrin-1 with the neuronal marker neurofilament (red) and yellow in the merged image. Bars, 100 µm. Results are representative of two separate experiments (n = 3 mice per group). (B) Leukocytes isolated from human peripheral blood were incubated with an antibody against netrin-1, CD15, CD14, CD68, CD3, CD19, and the expression of indicated marker was measured by flow cytometry. Results are representative of two separate experiments (from n = 3 healthy donors).
© Copyright Policy - openaccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4042652&req=5

fig5: Vagotomy reduces netrin-1 expression. Animals were subjected to unilateral vagotomy or a comparable sham surgical procedure and netrin-1 and neurofilament expression in lungs was assessed by immunofluorescence. (A) Colocalization (green) of netrin-1 with the neuronal marker neurofilament (red) and yellow in the merged image. Bars, 100 µm. Results are representative of two separate experiments (n = 3 mice per group). (B) Leukocytes isolated from human peripheral blood were incubated with an antibody against netrin-1, CD15, CD14, CD68, CD3, CD19, and the expression of indicated marker was measured by flow cytometry. Results are representative of two separate experiments (from n = 3 healthy donors).
Mentions: As we considered that the netrin-1 expression is likely regulated neuronally, we performed immunochemistry staining of lungs from vagotomized or sham-operated mice to determine netrin-1 expression. We identified reduced expression of netrin-1 within lungs from vagotomized mice, especially on neurofilament structures (Fig. 5 A).

Bottom Line: In netrin-1(+/-) mice, resolvin D1 (RvD1) was less effective in reducing neutrophil influx promoting resolution of peritonitis compared with Ntn1(+/+).Human monocytes incubated with netrin-1 produced proresolving mediators, including resolvins and lipoxins.Netrin-1 and RvD1 displayed bidirectional activation in that they stimulated each other's expression and enhanced efferocytosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Experimental Therapeutics and Reperfusion Injury, Harvard Institutes of Medicine, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115 Clinic of Anesthesiology and Intensive Care Medicine, University Hospital Tübingen, Eberhard-Karls University, 72076 Tübingen, Germany.

Show MeSH
Related in: MedlinePlus