Limits...
B lymphocytes undergo TLR2-dependent apoptosis upon Shigella infection.

Nothelfer K, Arena ET, Pinaud L, Neunlist M, Mozeleski B, Belotserkovsky I, Parsot C, Dinadayala P, Burger-Kentischer A, Raqib R, Sansonetti PJ, Phalipon A - J. Exp. Med. (2014)

Bottom Line: The induction of a type three secretion apparatus (T3SA)-dependent B cell death is observed in the human CL-01 B cell line in vitro, as well as in mouse B lymphocytes in vivo.The presence of bacterial co-signals is required to sensitize B cells to apoptosis and to up-regulate tlr2, thus enhancing IpaD binding.Apoptotic B lymphocytes in contact with Shigella-IpaD are detected in rectal biopsies of infected individuals.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institut Pasteur, INSERM U786, Unité de Pathogénie Microbienne Moléculaire, 75015 Paris, FranceInstitut Pasteur, INSERM U786, Unité de Pathogénie Microbienne Moléculaire, 75015 Paris, France.

Show MeSH

Related in: MedlinePlus

S. flexneri interacts with B lymphocytes and induces B cell apoptosis upon natural infection. Representative fluorescence microscopy images of ILFs in colonic biopsy tissue slides from two patients presenting severe shigellosis are shown. Bacteria were stained with an antibody against IpaD (red), B cells with anti-CD20cy (green), and apoptotic cells with a TUNEL kit (gray). Orthogonal slices are shown for each confocal image. Bars, 2 µm.
© Copyright Policy - openaccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4042640&req=5

fig9: S. flexneri interacts with B lymphocytes and induces B cell apoptosis upon natural infection. Representative fluorescence microscopy images of ILFs in colonic biopsy tissue slides from two patients presenting severe shigellosis are shown. Bacteria were stained with an antibody against IpaD (red), B cells with anti-CD20cy (green), and apoptotic cells with a TUNEL kit (gray). Orthogonal slices are shown for each confocal image. Bars, 2 µm.

Mentions: To assess Shigella-induced B cell apoptosis upon natural infection, we investigated S. flexneri–B lymphocyte interactions in patients diagnosed with shigellosis. Rectal biopsies from Shigella-infected patients presenting severe symptoms within 3–4 d of the onset of disease were chosen for immunohistochemistry analysis. As shown in Fig. 9, S. flexneri detected by using an anti-IpaD antibody was found in ILFs and in contact with apoptotic B lymphocytes, identified as TUNEL-positive cells. These findings suggest that S. flexneri–mediated B cell apoptosis indeed occurs in naturally infected patients.


B lymphocytes undergo TLR2-dependent apoptosis upon Shigella infection.

Nothelfer K, Arena ET, Pinaud L, Neunlist M, Mozeleski B, Belotserkovsky I, Parsot C, Dinadayala P, Burger-Kentischer A, Raqib R, Sansonetti PJ, Phalipon A - J. Exp. Med. (2014)

S. flexneri interacts with B lymphocytes and induces B cell apoptosis upon natural infection. Representative fluorescence microscopy images of ILFs in colonic biopsy tissue slides from two patients presenting severe shigellosis are shown. Bacteria were stained with an antibody against IpaD (red), B cells with anti-CD20cy (green), and apoptotic cells with a TUNEL kit (gray). Orthogonal slices are shown for each confocal image. Bars, 2 µm.
© Copyright Policy - openaccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4042640&req=5

fig9: S. flexneri interacts with B lymphocytes and induces B cell apoptosis upon natural infection. Representative fluorescence microscopy images of ILFs in colonic biopsy tissue slides from two patients presenting severe shigellosis are shown. Bacteria were stained with an antibody against IpaD (red), B cells with anti-CD20cy (green), and apoptotic cells with a TUNEL kit (gray). Orthogonal slices are shown for each confocal image. Bars, 2 µm.
Mentions: To assess Shigella-induced B cell apoptosis upon natural infection, we investigated S. flexneri–B lymphocyte interactions in patients diagnosed with shigellosis. Rectal biopsies from Shigella-infected patients presenting severe symptoms within 3–4 d of the onset of disease were chosen for immunohistochemistry analysis. As shown in Fig. 9, S. flexneri detected by using an anti-IpaD antibody was found in ILFs and in contact with apoptotic B lymphocytes, identified as TUNEL-positive cells. These findings suggest that S. flexneri–mediated B cell apoptosis indeed occurs in naturally infected patients.

Bottom Line: The induction of a type three secretion apparatus (T3SA)-dependent B cell death is observed in the human CL-01 B cell line in vitro, as well as in mouse B lymphocytes in vivo.The presence of bacterial co-signals is required to sensitize B cells to apoptosis and to up-regulate tlr2, thus enhancing IpaD binding.Apoptotic B lymphocytes in contact with Shigella-IpaD are detected in rectal biopsies of infected individuals.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institut Pasteur, INSERM U786, Unité de Pathogénie Microbienne Moléculaire, 75015 Paris, FranceInstitut Pasteur, INSERM U786, Unité de Pathogénie Microbienne Moléculaire, 75015 Paris, France.

Show MeSH
Related in: MedlinePlus