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Evaluation of bread crumbs as a potential carbon source for the growth of thraustochytrid species for oil and omega-3 production.

Thyagarajan T, Puri M, Vongsvivut J, Barrow CJ - Nutrients (2014)

Bottom Line: Static fermentation with 0.5% and 1% breadcrumbs resulted in 2.5 and 4.7 g/L of biomass, and 42.4 and 33.6 mg/g of saturated fatty acids, respectively.Scanning electron microscopic (SEM) studies confirmed the growth of both strains on breadcrumbs.The total lipid yield for static fermentation with bread crumbs was marginally lower than that of fermentation with glucose media, while the yield of unsaturated fatty acids was considerably lower, indicating that static fermentation may be more appropriate for the production of biodiesel than for the production of omega-3 rich oils in these strains.

View Article: PubMed Central - PubMed

Affiliation: Centre for Chemistry and Biotechnology, Deakin University, Pigdons Road, Waurn Ponds, Victoria 3217, Australia. tthyagar@deakin.edu.au.

ABSTRACT
The utilization of food waste by microorganisms to produce omega-3 fatty acids or biofuel is a potentially low cost method with positive environmental benefits. In the present study, the marine microorganisms Thraustochytrium sp. AH-2 and Schizochytrium sp. SR21 were used to evaluate the potential of breadcrumbs as an alternate carbon source for the production of lipids under static fermentation conditions. For the Thraustochytrium sp. AH-2, submerged liquid fermentation with 3% glucose produced 4.3 g/L of biomass and 44.16 mg/g of saturated fatty acids after seven days. Static fermentation with 0.5% and 1% breadcrumbs resulted in 2.5 and 4.7 g/L of biomass, and 42.4 and 33.6 mg/g of saturated fatty acids, respectively. Scanning electron microscopic (SEM) studies confirmed the growth of both strains on breadcrumbs. Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy for both strains were consistent with the utilization of breadcrumbs for the production of unsaturated lipids, albeit at relatively low levels. The total lipid yield for static fermentation with bread crumbs was marginally lower than that of fermentation with glucose media, while the yield of unsaturated fatty acids was considerably lower, indicating that static fermentation may be more appropriate for the production of biodiesel than for the production of omega-3 rich oils in these strains.

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SEM images of freeze-dried cells for (a) freeze-dried cells of Schizochytrium sp. SR21 grown under submerged liquid fermentation; (b) Schizochytrium sp. SR21 cells grown with 1% BC as alternate carbon source; (c) Thraustochytrium sp. AH-2 cells grown with 1% BC as alternate carbon source; (d) Thraustochytrium sp. AH-2 grown under submerged liquid fermentation.
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nutrients-06-02104-f002: SEM images of freeze-dried cells for (a) freeze-dried cells of Schizochytrium sp. SR21 grown under submerged liquid fermentation; (b) Schizochytrium sp. SR21 cells grown with 1% BC as alternate carbon source; (c) Thraustochytrium sp. AH-2 cells grown with 1% BC as alternate carbon source; (d) Thraustochytrium sp. AH-2 grown under submerged liquid fermentation.

Mentions: The fermentation growth for Thraustochytrium sp. AH-2 and Schizochytrium sp. SR21 were compared for BC and glucose as the carbon source using SEM. The morphology of freeze-dried unfermented BC was observed using SEM as a control material. The SEM images of cells grown using submerged liquid fermentation with glucose show spherical cells that are clumped together (Figure 2a). When grown in the presence of BC, cell clusters are attached to the BCs, confirming that cells do grow on this complex carbon source (Figure 2b,c).


Evaluation of bread crumbs as a potential carbon source for the growth of thraustochytrid species for oil and omega-3 production.

Thyagarajan T, Puri M, Vongsvivut J, Barrow CJ - Nutrients (2014)

SEM images of freeze-dried cells for (a) freeze-dried cells of Schizochytrium sp. SR21 grown under submerged liquid fermentation; (b) Schizochytrium sp. SR21 cells grown with 1% BC as alternate carbon source; (c) Thraustochytrium sp. AH-2 cells grown with 1% BC as alternate carbon source; (d) Thraustochytrium sp. AH-2 grown under submerged liquid fermentation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4042580&req=5

nutrients-06-02104-f002: SEM images of freeze-dried cells for (a) freeze-dried cells of Schizochytrium sp. SR21 grown under submerged liquid fermentation; (b) Schizochytrium sp. SR21 cells grown with 1% BC as alternate carbon source; (c) Thraustochytrium sp. AH-2 cells grown with 1% BC as alternate carbon source; (d) Thraustochytrium sp. AH-2 grown under submerged liquid fermentation.
Mentions: The fermentation growth for Thraustochytrium sp. AH-2 and Schizochytrium sp. SR21 were compared for BC and glucose as the carbon source using SEM. The morphology of freeze-dried unfermented BC was observed using SEM as a control material. The SEM images of cells grown using submerged liquid fermentation with glucose show spherical cells that are clumped together (Figure 2a). When grown in the presence of BC, cell clusters are attached to the BCs, confirming that cells do grow on this complex carbon source (Figure 2b,c).

Bottom Line: Static fermentation with 0.5% and 1% breadcrumbs resulted in 2.5 and 4.7 g/L of biomass, and 42.4 and 33.6 mg/g of saturated fatty acids, respectively.Scanning electron microscopic (SEM) studies confirmed the growth of both strains on breadcrumbs.The total lipid yield for static fermentation with bread crumbs was marginally lower than that of fermentation with glucose media, while the yield of unsaturated fatty acids was considerably lower, indicating that static fermentation may be more appropriate for the production of biodiesel than for the production of omega-3 rich oils in these strains.

View Article: PubMed Central - PubMed

Affiliation: Centre for Chemistry and Biotechnology, Deakin University, Pigdons Road, Waurn Ponds, Victoria 3217, Australia. tthyagar@deakin.edu.au.

ABSTRACT
The utilization of food waste by microorganisms to produce omega-3 fatty acids or biofuel is a potentially low cost method with positive environmental benefits. In the present study, the marine microorganisms Thraustochytrium sp. AH-2 and Schizochytrium sp. SR21 were used to evaluate the potential of breadcrumbs as an alternate carbon source for the production of lipids under static fermentation conditions. For the Thraustochytrium sp. AH-2, submerged liquid fermentation with 3% glucose produced 4.3 g/L of biomass and 44.16 mg/g of saturated fatty acids after seven days. Static fermentation with 0.5% and 1% breadcrumbs resulted in 2.5 and 4.7 g/L of biomass, and 42.4 and 33.6 mg/g of saturated fatty acids, respectively. Scanning electron microscopic (SEM) studies confirmed the growth of both strains on breadcrumbs. Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy for both strains were consistent with the utilization of breadcrumbs for the production of unsaturated lipids, albeit at relatively low levels. The total lipid yield for static fermentation with bread crumbs was marginally lower than that of fermentation with glucose media, while the yield of unsaturated fatty acids was considerably lower, indicating that static fermentation may be more appropriate for the production of biodiesel than for the production of omega-3 rich oils in these strains.

Show MeSH
Related in: MedlinePlus