Limits...
ShaoYao decoction ameliorates colitis-associated colorectal cancer by downregulating proinflammatory cytokines and promoting epithelial-mesenchymal transition.

Lin X, Yi Z, Diao J, Shao M, Zhao L, Cai H, Fan Q, Yao X, Sun X - J Transl Med (2014)

Bottom Line: In this study, azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colitis-associated CRC (caCRC) model and CRC cell lines were used to examine the effects of SYD on CRC in vivo and in vitro.SYD significantly increased the survival rate of the mice, ameliorated the general well-being of the mice, and reduced the incidence and multiplicity of colonic neoplasms.SYD reduced the expression levels of serum interleukin 1β, interleukin-6, tumor necrosis factor α, tumor-associated macrophages, and p65.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. yjb9211@21cn.com.

ABSTRACT

Background: Shaoyao decoction (SYD) is a traditional Chinese medicine prescription formulated by Liu Wan-Su, a master of traditional Chinese medicine in Jin-Yuan Dynasty. SYD is effective in treating ulcerative colitis. Paeonol, a component of SYD, inhibits colorectal cancer (CRC) cell proliferation and induces CRC cell apoptosis. In this study, azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colitis-associated CRC (caCRC) model and CRC cell lines were used to examine the effects of SYD on CRC in vivo and in vitro.

Methods: A translational medicine strategy based on phytomics quality control was adopted. Liquid chromatography was employed for the chemical characterization and chemical fingerprinting of SYD. Protein expression and macrophage existence were determined by immunohistochemistry and western blot. Serum cytokines were quantified by Luminex assay.

Results: AOM/DSS-induced caCRC phenotypically resembled human caCRC. SYD significantly increased the survival rate of the mice, ameliorated the general well-being of the mice, and reduced the incidence and multiplicity of colonic neoplasms. SYD inhibited epithelial-mesenchymal transition (EMT), as indicated by upregulated epithelia cadherin and downregulated neuronal cadherin, fibronectin, vimentin, and transcription factor Snail. SYD reduced the expression levels of serum interleukin 1β, interleukin-6, tumor necrosis factor α, tumor-associated macrophages, and p65. These results showed that SYD can attenuate proinflammatory cytokines and inhibit EMT.

Conclusions: SYD ameliorates caCRC by suppressing inflammation and inhibiting EMT. SYD might be an alternative therapy for caCRC.

Show MeSH

Related in: MedlinePlus

Effects of SYD on TAM and cytokine profile.(A) Immunohistochemical staining of NF-κB and F4/80 in tumor tissues or normal colonic tissues .200 × for all, scale bar = 100 μm. High expression levels of NF-κB and F4/80 were detected in the model group. SYD decreased NF-κB expression and F4/80-positive cells. Semiquantitative analysis of these proteins is shown in Table 4. (B) Luminex assay of IL-1β, IL-6, and TNF-α levels in serum. Serum contents of IL-1β, IL-6, and TNF-α were reduced by SYD. Data are presented as mean ± SD. *P < 0.01 vs. model, #P < 0.05 vs. model; △P < 0.01 vs. control, ▲P < 0.0 5 vs. control. (C) A model of SYD function in caCRC.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4022058&req=5

Figure 7: Effects of SYD on TAM and cytokine profile.(A) Immunohistochemical staining of NF-κB and F4/80 in tumor tissues or normal colonic tissues .200 × for all, scale bar = 100 μm. High expression levels of NF-κB and F4/80 were detected in the model group. SYD decreased NF-κB expression and F4/80-positive cells. Semiquantitative analysis of these proteins is shown in Table 4. (B) Luminex assay of IL-1β, IL-6, and TNF-α levels in serum. Serum contents of IL-1β, IL-6, and TNF-α were reduced by SYD. Data are presented as mean ± SD. *P < 0.01 vs. model, #P < 0.05 vs. model; △P < 0.01 vs. control, ▲P < 0.0 5 vs. control. (C) A model of SYD function in caCRC.

Mentions: The number of macrophages and the expression of NF-κB were increased significantly in the model group compared with those in the control group; SYD counteracted these effects (Figure 7A). Serum IL-1β, IL-6, and TNF-α were upregulated in the model group and were significantly inhibited by SYD (Figure 7B).


ShaoYao decoction ameliorates colitis-associated colorectal cancer by downregulating proinflammatory cytokines and promoting epithelial-mesenchymal transition.

Lin X, Yi Z, Diao J, Shao M, Zhao L, Cai H, Fan Q, Yao X, Sun X - J Transl Med (2014)

Effects of SYD on TAM and cytokine profile.(A) Immunohistochemical staining of NF-κB and F4/80 in tumor tissues or normal colonic tissues .200 × for all, scale bar = 100 μm. High expression levels of NF-κB and F4/80 were detected in the model group. SYD decreased NF-κB expression and F4/80-positive cells. Semiquantitative analysis of these proteins is shown in Table 4. (B) Luminex assay of IL-1β, IL-6, and TNF-α levels in serum. Serum contents of IL-1β, IL-6, and TNF-α were reduced by SYD. Data are presented as mean ± SD. *P < 0.01 vs. model, #P < 0.05 vs. model; △P < 0.01 vs. control, ▲P < 0.0 5 vs. control. (C) A model of SYD function in caCRC.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4022058&req=5

Figure 7: Effects of SYD on TAM and cytokine profile.(A) Immunohistochemical staining of NF-κB and F4/80 in tumor tissues or normal colonic tissues .200 × for all, scale bar = 100 μm. High expression levels of NF-κB and F4/80 were detected in the model group. SYD decreased NF-κB expression and F4/80-positive cells. Semiquantitative analysis of these proteins is shown in Table 4. (B) Luminex assay of IL-1β, IL-6, and TNF-α levels in serum. Serum contents of IL-1β, IL-6, and TNF-α were reduced by SYD. Data are presented as mean ± SD. *P < 0.01 vs. model, #P < 0.05 vs. model; △P < 0.01 vs. control, ▲P < 0.0 5 vs. control. (C) A model of SYD function in caCRC.
Mentions: The number of macrophages and the expression of NF-κB were increased significantly in the model group compared with those in the control group; SYD counteracted these effects (Figure 7A). Serum IL-1β, IL-6, and TNF-α were upregulated in the model group and were significantly inhibited by SYD (Figure 7B).

Bottom Line: In this study, azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colitis-associated CRC (caCRC) model and CRC cell lines were used to examine the effects of SYD on CRC in vivo and in vitro.SYD significantly increased the survival rate of the mice, ameliorated the general well-being of the mice, and reduced the incidence and multiplicity of colonic neoplasms.SYD reduced the expression levels of serum interleukin 1β, interleukin-6, tumor necrosis factor α, tumor-associated macrophages, and p65.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. yjb9211@21cn.com.

ABSTRACT

Background: Shaoyao decoction (SYD) is a traditional Chinese medicine prescription formulated by Liu Wan-Su, a master of traditional Chinese medicine in Jin-Yuan Dynasty. SYD is effective in treating ulcerative colitis. Paeonol, a component of SYD, inhibits colorectal cancer (CRC) cell proliferation and induces CRC cell apoptosis. In this study, azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colitis-associated CRC (caCRC) model and CRC cell lines were used to examine the effects of SYD on CRC in vivo and in vitro.

Methods: A translational medicine strategy based on phytomics quality control was adopted. Liquid chromatography was employed for the chemical characterization and chemical fingerprinting of SYD. Protein expression and macrophage existence were determined by immunohistochemistry and western blot. Serum cytokines were quantified by Luminex assay.

Results: AOM/DSS-induced caCRC phenotypically resembled human caCRC. SYD significantly increased the survival rate of the mice, ameliorated the general well-being of the mice, and reduced the incidence and multiplicity of colonic neoplasms. SYD inhibited epithelial-mesenchymal transition (EMT), as indicated by upregulated epithelia cadherin and downregulated neuronal cadherin, fibronectin, vimentin, and transcription factor Snail. SYD reduced the expression levels of serum interleukin 1β, interleukin-6, tumor necrosis factor α, tumor-associated macrophages, and p65. These results showed that SYD can attenuate proinflammatory cytokines and inhibit EMT.

Conclusions: SYD ameliorates caCRC by suppressing inflammation and inhibiting EMT. SYD might be an alternative therapy for caCRC.

Show MeSH
Related in: MedlinePlus