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Tertiary-amine functionalized polyplexes enhanced cellular uptake and prolonged gene expression.

Lo CW, Chang Y, Lee JL, Tsai WB, Chen WS - PLoS ONE (2014)

Bottom Line: Its in-vitro and in-vivo effects on the transfection efficiency and the expression duration were evaluated.However, effective transfection only occurred in the US groups in vivo.Lower weight ratios, e.g., 0.25, exhibited better in-vivo expression for at least 45 days.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, ROC.

ABSTRACT
Ultrasound (US) has been found to facilitate the transport of DNA across cell membranes. However, the transfection efficiency is generally low, and the expression duration of the transfected gene is brief. In this study, a tertiary polycation, Poly(2-(dimethylamino) ethyl methacrylate) (PDMAEMA), was used as a carrier for US-mediated gene transfection. Its in-vitro and in-vivo effects on the transfection efficiency and the expression duration were evaluated. A mixture of pCI-neo-luc and PDMAEMA was transfected to cultured cells or mouse muscle by exposure to 1-MHz pulse US. A strong expression of luciferase was found 10 days after the transfection in vitro regardless of US exposure. However, effective transfection only occurred in the US groups in vivo. The transfection ability depended on the weight ratio of PDMAEMA to DNA, and was different for the in-vitro and in-vivo conditions. Lower weight ratios, e.g., 0.25, exhibited better in-vivo expression for at least 45 days.

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Related in: MedlinePlus

Schematic of in-vivo and in-vitro experimental setup.
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pone-0097627-g001: Schematic of in-vivo and in-vitro experimental setup.

Mentions: Schematic diagrams of in-vivo and in-vitro experimental setup are shown in Figure 1.


Tertiary-amine functionalized polyplexes enhanced cellular uptake and prolonged gene expression.

Lo CW, Chang Y, Lee JL, Tsai WB, Chen WS - PLoS ONE (2014)

Schematic of in-vivo and in-vitro experimental setup.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4020921&req=5

pone-0097627-g001: Schematic of in-vivo and in-vitro experimental setup.
Mentions: Schematic diagrams of in-vivo and in-vitro experimental setup are shown in Figure 1.

Bottom Line: Its in-vitro and in-vivo effects on the transfection efficiency and the expression duration were evaluated.However, effective transfection only occurred in the US groups in vivo.Lower weight ratios, e.g., 0.25, exhibited better in-vivo expression for at least 45 days.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, ROC.

ABSTRACT
Ultrasound (US) has been found to facilitate the transport of DNA across cell membranes. However, the transfection efficiency is generally low, and the expression duration of the transfected gene is brief. In this study, a tertiary polycation, Poly(2-(dimethylamino) ethyl methacrylate) (PDMAEMA), was used as a carrier for US-mediated gene transfection. Its in-vitro and in-vivo effects on the transfection efficiency and the expression duration were evaluated. A mixture of pCI-neo-luc and PDMAEMA was transfected to cultured cells or mouse muscle by exposure to 1-MHz pulse US. A strong expression of luciferase was found 10 days after the transfection in vitro regardless of US exposure. However, effective transfection only occurred in the US groups in vivo. The transfection ability depended on the weight ratio of PDMAEMA to DNA, and was different for the in-vitro and in-vivo conditions. Lower weight ratios, e.g., 0.25, exhibited better in-vivo expression for at least 45 days.

Show MeSH
Related in: MedlinePlus