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Protective Effects of Bu-Shen-Huo-Xue Formula against 5/6 Nephrectomy-Induced Chronic Renal Failure in Rats.

Lu JR, Han HY, Chen J, Xiong CX, Wang XH, Hu J, Chen XF, Ma L - Evid Based Complement Alternat Med (2014)

Bottom Line: Bu-Shen-Huo-Xue formula (BSHX), one of traditional herbal formulations, has been clinically employed to treat CRF for decades, but the mechanisms involved have not been investigated.After eight weeks of treatment, BSHX significantly decreased the levels of Scr and BUN, downregulated the mRNA expression levels of TGF-β 1, CTGF, NF-κB, TNF-α, and OPN, upregulated the mRNA expression of PPARγ, and reduced in situ expression of fibronectin and laminins.Histological findings also showed significant amelioration of the damaged renal tissue.

View Article: PubMed Central - PubMed

Affiliation: Division of Nephrology, Shanghai Seventh People's Hospital, Shanghai 200137, China.

ABSTRACT
Chronic renal failure (CRF) is a serious disease related to increasing incidence and prevalence as well as decline in quality of life. Bu-Shen-Huo-Xue formula (BSHX), one of traditional herbal formulations, has been clinically employed to treat CRF for decades, but the mechanisms involved have not been investigated. In the present study, we investigated the effects of BSHX on some closely related parameters in 5/6 nephrectomy CRF rats. Rats with CRF were divided into five groups, namely, one control group, one enalapril group, and three BSHX treatment groups (0.25, 0.5, and 1 g/kg·d). The rats subjected to sham operation were used as a normal control. After eight weeks of treatment, BSHX significantly decreased the levels of Scr and BUN, downregulated the mRNA expression levels of TGF-β 1, CTGF, NF-κB, TNF-α, and OPN, upregulated the mRNA expression of PPARγ, and reduced in situ expression of fibronectin and laminins. Histological findings also showed significant amelioration of the damaged renal tissue. BSHX protects 5/6 nephrectomy rats against chronic renal failure probably via regulating the expression of TNF-α, NF-κB, TGF-β 1, CTGF, PPARγ, OPN, fibronectin, and laminins and is useful for therapy of CRF.

No MeSH data available.


Related in: MedlinePlus

Effects of BSHX on mRNA expression of PPARγ, NF-κB, and TNF-α in renal tissue of 5/6 nephrectomy rats. After treatment with BSHX at the doses of 0.25, 0.5, and 1.0 g/kg·d for eight successive weeks, the mRNA expression of PPARγ (a), NF-κB (b), and TNF-α (c) in the renal tissue was analyzed by FQ-RT-PCR. ΔΔP < 0.01 compared with the sham group. **P < 0.01 compared with the control group. Data are expressed as the mean ± SD. n = 6.
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fig4: Effects of BSHX on mRNA expression of PPARγ, NF-κB, and TNF-α in renal tissue of 5/6 nephrectomy rats. After treatment with BSHX at the doses of 0.25, 0.5, and 1.0 g/kg·d for eight successive weeks, the mRNA expression of PPARγ (a), NF-κB (b), and TNF-α (c) in the renal tissue was analyzed by FQ-RT-PCR. ΔΔP < 0.01 compared with the sham group. **P < 0.01 compared with the control group. Data are expressed as the mean ± SD. n = 6.

Mentions: Figure 4 shows that there was an obvious decrease in PPARγ but a significant increase in NF-κB and TNF-α in the control group of rats when compared with the sham group of rats. However, PPARγ was elevated and TNF-α was decreased evidently and dose-dependently by eight consecutive weeks of BSHX treatment. The mRNA expression of NF-κB was also suppressed markedly but not dose-dependently.


Protective Effects of Bu-Shen-Huo-Xue Formula against 5/6 Nephrectomy-Induced Chronic Renal Failure in Rats.

Lu JR, Han HY, Chen J, Xiong CX, Wang XH, Hu J, Chen XF, Ma L - Evid Based Complement Alternat Med (2014)

Effects of BSHX on mRNA expression of PPARγ, NF-κB, and TNF-α in renal tissue of 5/6 nephrectomy rats. After treatment with BSHX at the doses of 0.25, 0.5, and 1.0 g/kg·d for eight successive weeks, the mRNA expression of PPARγ (a), NF-κB (b), and TNF-α (c) in the renal tissue was analyzed by FQ-RT-PCR. ΔΔP < 0.01 compared with the sham group. **P < 0.01 compared with the control group. Data are expressed as the mean ± SD. n = 6.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4020566&req=5

fig4: Effects of BSHX on mRNA expression of PPARγ, NF-κB, and TNF-α in renal tissue of 5/6 nephrectomy rats. After treatment with BSHX at the doses of 0.25, 0.5, and 1.0 g/kg·d for eight successive weeks, the mRNA expression of PPARγ (a), NF-κB (b), and TNF-α (c) in the renal tissue was analyzed by FQ-RT-PCR. ΔΔP < 0.01 compared with the sham group. **P < 0.01 compared with the control group. Data are expressed as the mean ± SD. n = 6.
Mentions: Figure 4 shows that there was an obvious decrease in PPARγ but a significant increase in NF-κB and TNF-α in the control group of rats when compared with the sham group of rats. However, PPARγ was elevated and TNF-α was decreased evidently and dose-dependently by eight consecutive weeks of BSHX treatment. The mRNA expression of NF-κB was also suppressed markedly but not dose-dependently.

Bottom Line: Bu-Shen-Huo-Xue formula (BSHX), one of traditional herbal formulations, has been clinically employed to treat CRF for decades, but the mechanisms involved have not been investigated.After eight weeks of treatment, BSHX significantly decreased the levels of Scr and BUN, downregulated the mRNA expression levels of TGF-β 1, CTGF, NF-κB, TNF-α, and OPN, upregulated the mRNA expression of PPARγ, and reduced in situ expression of fibronectin and laminins.Histological findings also showed significant amelioration of the damaged renal tissue.

View Article: PubMed Central - PubMed

Affiliation: Division of Nephrology, Shanghai Seventh People's Hospital, Shanghai 200137, China.

ABSTRACT
Chronic renal failure (CRF) is a serious disease related to increasing incidence and prevalence as well as decline in quality of life. Bu-Shen-Huo-Xue formula (BSHX), one of traditional herbal formulations, has been clinically employed to treat CRF for decades, but the mechanisms involved have not been investigated. In the present study, we investigated the effects of BSHX on some closely related parameters in 5/6 nephrectomy CRF rats. Rats with CRF were divided into five groups, namely, one control group, one enalapril group, and three BSHX treatment groups (0.25, 0.5, and 1 g/kg·d). The rats subjected to sham operation were used as a normal control. After eight weeks of treatment, BSHX significantly decreased the levels of Scr and BUN, downregulated the mRNA expression levels of TGF-β 1, CTGF, NF-κB, TNF-α, and OPN, upregulated the mRNA expression of PPARγ, and reduced in situ expression of fibronectin and laminins. Histological findings also showed significant amelioration of the damaged renal tissue. BSHX protects 5/6 nephrectomy rats against chronic renal failure probably via regulating the expression of TNF-α, NF-κB, TGF-β 1, CTGF, PPARγ, OPN, fibronectin, and laminins and is useful for therapy of CRF.

No MeSH data available.


Related in: MedlinePlus