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The osteogenesis of bone marrow stem cells on mPEG-PCL-mPEG/hydroxyapatite composite scaffold via solid freeform fabrication.

Liao HT, Chen YY, Lai YT, Hsieh MF, Jiang CP - Biomed Res Int (2014)

Bottom Line: However, the in vivo bone forming efficacy between PBMSCs seeded PCL and PCL/HA scaffold was different from the in vitro results.The outcome indicated that the PCL/HA scaffold which had bone-mimetic environment due to the addition of HA resulted in better bone regeneration and mechanical strength than those of PCL scaffold.Therefore, providing a bone-mimetic scaffold is another crucial factor for bone tissue engineering in addition to the biocompatibility, 3D architecture with high porosity, and interpored connection.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Reconstructive Surgery, Craniofacial Research Center, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kweishan, Taoyuan 333, Taiwan ; Department of Chemical and Materials Engineering, Chang Gung University, Kweishan, Taoyuan 333, Taiwan.

ABSTRACT
The study described a novel bone tissue scaffold fabricated by computer-aided, air pressure-aided deposition system to control the macro- and microstructure precisely. The porcine bone marrow stem cells (PBMSCs) seeded on either mPEG-PCL-mPEG (PCL) or mPEG-PCL-mPEG/hydroxyapatite (PCL/HA) composite scaffold were cultured under osteogenic medium to test the ability of osteogenesis in vitro. The experimental outcomes indicated that both scaffolds possessed adequate pore size, porosity, and hydrophilicity for the attachment and proliferation of PBMSCs and the PBMSCs expressed upregulated genes of osteogensis and angiogenesis in similar manner on both scaffolds. The major differences between these two types of the scaffolds were the addition of HA leading to higher hardness of PCL/HA scaffold, cell proliferation, and VEGF gene expression in PCL/HA scaffold. However, the in vivo bone forming efficacy between PBMSCs seeded PCL and PCL/HA scaffold was different from the in vitro results. The outcome indicated that the PCL/HA scaffold which had bone-mimetic environment due to the addition of HA resulted in better bone regeneration and mechanical strength than those of PCL scaffold. Therefore, providing a bone-mimetic scaffold is another crucial factor for bone tissue engineering in addition to the biocompatibility, 3D architecture with high porosity, and interpored connection.

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Related in: MedlinePlus

The histological examination of specimens post-op 6 months for PCL/PBMSCs: (a) H&E (40X), (c) H&E 100X, (e) Masson's trichrome stain (40X), and (g) Masson's trichrome stain (100X) and PCL/HA/PBMSCs group: (b) H&E (40X), (d) H&E 100X, (f) Masson's trichrome stain (40X), and (h) Masson's trichrome stain (100X). S: scaffold; B: bone formation area (deep blue area); F: fibrotic tissue area (light blue area). Black scale bar at 40X is 200 μm and black scale bar at 100X is 100 μm.
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fig10: The histological examination of specimens post-op 6 months for PCL/PBMSCs: (a) H&E (40X), (c) H&E 100X, (e) Masson's trichrome stain (40X), and (g) Masson's trichrome stain (100X) and PCL/HA/PBMSCs group: (b) H&E (40X), (d) H&E 100X, (f) Masson's trichrome stain (40X), and (h) Masson's trichrome stain (100X). S: scaffold; B: bone formation area (deep blue area); F: fibrotic tissue area (light blue area). Black scale bar at 40X is 200 μm and black scale bar at 100X is 100 μm.

Mentions: The pigs were sacrificed at post-op 6 mo and the implant tissue-engineered bone construct was harvested for the mechanical strength and histological analysis. The Vickers diamond microhardness test revealed that the PCL/HA group had significantly higher mechanical strength than PCL group (Figure 9). The histology also showed more bone formation (deep blue area in Masson's trichrome stain) within the pore of scaffold at PCL/HA group (Figures 10(b), 10(d), 10(f), and 10(h)) than PCL group (Figures 10(a), 10(c), 10(e), and 10(g)) by both H&E and Masson's trichrome stains.


The osteogenesis of bone marrow stem cells on mPEG-PCL-mPEG/hydroxyapatite composite scaffold via solid freeform fabrication.

Liao HT, Chen YY, Lai YT, Hsieh MF, Jiang CP - Biomed Res Int (2014)

The histological examination of specimens post-op 6 months for PCL/PBMSCs: (a) H&E (40X), (c) H&E 100X, (e) Masson's trichrome stain (40X), and (g) Masson's trichrome stain (100X) and PCL/HA/PBMSCs group: (b) H&E (40X), (d) H&E 100X, (f) Masson's trichrome stain (40X), and (h) Masson's trichrome stain (100X). S: scaffold; B: bone formation area (deep blue area); F: fibrotic tissue area (light blue area). Black scale bar at 40X is 200 μm and black scale bar at 100X is 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4020560&req=5

fig10: The histological examination of specimens post-op 6 months for PCL/PBMSCs: (a) H&E (40X), (c) H&E 100X, (e) Masson's trichrome stain (40X), and (g) Masson's trichrome stain (100X) and PCL/HA/PBMSCs group: (b) H&E (40X), (d) H&E 100X, (f) Masson's trichrome stain (40X), and (h) Masson's trichrome stain (100X). S: scaffold; B: bone formation area (deep blue area); F: fibrotic tissue area (light blue area). Black scale bar at 40X is 200 μm and black scale bar at 100X is 100 μm.
Mentions: The pigs were sacrificed at post-op 6 mo and the implant tissue-engineered bone construct was harvested for the mechanical strength and histological analysis. The Vickers diamond microhardness test revealed that the PCL/HA group had significantly higher mechanical strength than PCL group (Figure 9). The histology also showed more bone formation (deep blue area in Masson's trichrome stain) within the pore of scaffold at PCL/HA group (Figures 10(b), 10(d), 10(f), and 10(h)) than PCL group (Figures 10(a), 10(c), 10(e), and 10(g)) by both H&E and Masson's trichrome stains.

Bottom Line: However, the in vivo bone forming efficacy between PBMSCs seeded PCL and PCL/HA scaffold was different from the in vitro results.The outcome indicated that the PCL/HA scaffold which had bone-mimetic environment due to the addition of HA resulted in better bone regeneration and mechanical strength than those of PCL scaffold.Therefore, providing a bone-mimetic scaffold is another crucial factor for bone tissue engineering in addition to the biocompatibility, 3D architecture with high porosity, and interpored connection.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Reconstructive Surgery, Craniofacial Research Center, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kweishan, Taoyuan 333, Taiwan ; Department of Chemical and Materials Engineering, Chang Gung University, Kweishan, Taoyuan 333, Taiwan.

ABSTRACT
The study described a novel bone tissue scaffold fabricated by computer-aided, air pressure-aided deposition system to control the macro- and microstructure precisely. The porcine bone marrow stem cells (PBMSCs) seeded on either mPEG-PCL-mPEG (PCL) or mPEG-PCL-mPEG/hydroxyapatite (PCL/HA) composite scaffold were cultured under osteogenic medium to test the ability of osteogenesis in vitro. The experimental outcomes indicated that both scaffolds possessed adequate pore size, porosity, and hydrophilicity for the attachment and proliferation of PBMSCs and the PBMSCs expressed upregulated genes of osteogensis and angiogenesis in similar manner on both scaffolds. The major differences between these two types of the scaffolds were the addition of HA leading to higher hardness of PCL/HA scaffold, cell proliferation, and VEGF gene expression in PCL/HA scaffold. However, the in vivo bone forming efficacy between PBMSCs seeded PCL and PCL/HA scaffold was different from the in vitro results. The outcome indicated that the PCL/HA scaffold which had bone-mimetic environment due to the addition of HA resulted in better bone regeneration and mechanical strength than those of PCL scaffold. Therefore, providing a bone-mimetic scaffold is another crucial factor for bone tissue engineering in addition to the biocompatibility, 3D architecture with high porosity, and interpored connection.

Show MeSH
Related in: MedlinePlus