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Parametric Optimization of Cultural Conditions for Carboxymethyl Cellulase Production Using Pretreated Rice Straw by Bacillus sp. 313SI under Stationary and Shaking Conditions.

Goyal V, Mittal A, Bhuwal AK, Singh G, Yadav A, Aggarwal NK - Biotechnol Res Int (2014)

Bottom Line: Carboxymethyl cellulase (CMCase) provides a key opportunity for achieving tremendous benefits of utilizing rice straw as cellulosic biomass.For maximum titre of CMCase carboxymethyl cellulose was optimized as the best carbon source under both cultural conditions while ammonium sulphate and ammonium nitrate were optimized as the best nitrogen sources under stationary and shaking conditions, respectively.The present study provides the useful data about the optimized conditions for CMCase production by Bacillus sp. 313SI from pretreated rice straw.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Kurukshetra University, Kurukshetra, Haryana 136119, India.

ABSTRACT
Carboxymethyl cellulase (CMCase) provides a key opportunity for achieving tremendous benefits of utilizing rice straw as cellulosic biomass. Out of total 80 microbial isolates from different ecological niches one bacterial strain, identified as Bacillus sp. 313SI, was selected for CMCase production under stationary as well as shaking conditions of growth. During two-stage pretreatment, rice straw was first treated with 0.5 M KOH to remove lignin followed by treatment with 0.1 N H2SO4 for removal of hemicellulose. The maximum carboxymethyl cellulase activity of 3.08 U/mL was obtained using 1% (w/v) pretreated rice straw with 1% (v/v) inoculum, pH 8.0 at 35°C after 60 h of growth under stationary conditions, while the same was obtained as 4.15 U/mL using 0.75% (w/v) pretreated substrate with 0.4% (v/v) inoculum, pH 8.0 at 30°C, under shaking conditions of growth for 48 h. For maximum titre of CMCase carboxymethyl cellulose was optimized as the best carbon source under both cultural conditions while ammonium sulphate and ammonium nitrate were optimized as the best nitrogen sources under stationary and shaking conditions, respectively. The present study provides the useful data about the optimized conditions for CMCase production by Bacillus sp. 313SI from pretreated rice straw.

No MeSH data available.


Related in: MedlinePlus

Effect of different carbon sources on carboxymethyl cellulase enzyme production by Bacillus sp. 313SI under stationary and shaking conditions of growth. Values in figure are means of three replicates with standard deviation.
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fig6: Effect of different carbon sources on carboxymethyl cellulase enzyme production by Bacillus sp. 313SI under stationary and shaking conditions of growth. Values in figure are means of three replicates with standard deviation.

Mentions: Supplementation of carbon and nitrogen sources in medium showed a significant increase in carboxymethyl cellulase enzyme production by Bacillus sp. 313SI from pretreated rice straw under stationary and shaking conditions. Effect of different carbon sources (0.1% w/v) on the production of carboxymethyl cellulase enzyme was evaluated as shown in Figure 6.


Parametric Optimization of Cultural Conditions for Carboxymethyl Cellulase Production Using Pretreated Rice Straw by Bacillus sp. 313SI under Stationary and Shaking Conditions.

Goyal V, Mittal A, Bhuwal AK, Singh G, Yadav A, Aggarwal NK - Biotechnol Res Int (2014)

Effect of different carbon sources on carboxymethyl cellulase enzyme production by Bacillus sp. 313SI under stationary and shaking conditions of growth. Values in figure are means of three replicates with standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4020544&req=5

fig6: Effect of different carbon sources on carboxymethyl cellulase enzyme production by Bacillus sp. 313SI under stationary and shaking conditions of growth. Values in figure are means of three replicates with standard deviation.
Mentions: Supplementation of carbon and nitrogen sources in medium showed a significant increase in carboxymethyl cellulase enzyme production by Bacillus sp. 313SI from pretreated rice straw under stationary and shaking conditions. Effect of different carbon sources (0.1% w/v) on the production of carboxymethyl cellulase enzyme was evaluated as shown in Figure 6.

Bottom Line: Carboxymethyl cellulase (CMCase) provides a key opportunity for achieving tremendous benefits of utilizing rice straw as cellulosic biomass.For maximum titre of CMCase carboxymethyl cellulose was optimized as the best carbon source under both cultural conditions while ammonium sulphate and ammonium nitrate were optimized as the best nitrogen sources under stationary and shaking conditions, respectively.The present study provides the useful data about the optimized conditions for CMCase production by Bacillus sp. 313SI from pretreated rice straw.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Kurukshetra University, Kurukshetra, Haryana 136119, India.

ABSTRACT
Carboxymethyl cellulase (CMCase) provides a key opportunity for achieving tremendous benefits of utilizing rice straw as cellulosic biomass. Out of total 80 microbial isolates from different ecological niches one bacterial strain, identified as Bacillus sp. 313SI, was selected for CMCase production under stationary as well as shaking conditions of growth. During two-stage pretreatment, rice straw was first treated with 0.5 M KOH to remove lignin followed by treatment with 0.1 N H2SO4 for removal of hemicellulose. The maximum carboxymethyl cellulase activity of 3.08 U/mL was obtained using 1% (w/v) pretreated rice straw with 1% (v/v) inoculum, pH 8.0 at 35°C after 60 h of growth under stationary conditions, while the same was obtained as 4.15 U/mL using 0.75% (w/v) pretreated substrate with 0.4% (v/v) inoculum, pH 8.0 at 30°C, under shaking conditions of growth for 48 h. For maximum titre of CMCase carboxymethyl cellulose was optimized as the best carbon source under both cultural conditions while ammonium sulphate and ammonium nitrate were optimized as the best nitrogen sources under stationary and shaking conditions, respectively. The present study provides the useful data about the optimized conditions for CMCase production by Bacillus sp. 313SI from pretreated rice straw.

No MeSH data available.


Related in: MedlinePlus