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Nuclear distribution of RNA polymerase II and mRNA processing machinery in early mammalian embryos.

Bogolyubova IO, Bogolyubov DS - Biomed Res Int (2014)

Bottom Line: Spatial distribution of components of nuclear metabolism provides a significant impact on regulation of the processes of gene expression.However, the period of cleavage is characterized by the most drastic and dynamic nuclear reorganizations accompanying zygotic gene activation.In this minireview, we try to summarize the results of studies concerning distribution of major factors involved in RNA polymerase II-dependent transcription, pre-mRNA splicing mRNA export that have been carried out on early embryos of mammals.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cell Morphology, Institute of Cytology RAS, 4 Tikhoretsky Avenue, St. Petersburg 194064, Russia.

ABSTRACT
Spatial distribution of components of nuclear metabolism provides a significant impact on regulation of the processes of gene expression. While distribution of the key nuclear antigens and their association with the defined nuclear domains were thoroughly traced in mammalian somatic cells, similar data for the preimplantation embryos are scanty and fragmental. However, the period of cleavage is characterized by the most drastic and dynamic nuclear reorganizations accompanying zygotic gene activation. In this minireview, we try to summarize the results of studies concerning distribution of major factors involved in RNA polymerase II-dependent transcription, pre-mRNA splicing mRNA export that have been carried out on early embryos of mammals.

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Double immunolocalization of SC35 (a), (b) and transcription factor TFIID (a′), (b′) in mouse embryos. TFIID is revealed in the nuclei at all studied stages. Colocalization of SC35 and TFIID is intensified during ZGA. Bar is 10 μm, according to [24], open access.
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fig3: Double immunolocalization of SC35 (a), (b) and transcription factor TFIID (a′), (b′) in mouse embryos. TFIID is revealed in the nuclei at all studied stages. Colocalization of SC35 and TFIID is intensified during ZGA. Bar is 10 μm, according to [24], open access.

Mentions: In mouse embryo pronuclei, basal transcription factor TFIID has been revealed before the onset of ZGA in association with nuclear speckles already at the zygote stage [24]. During realization of ZGA, the presence of TFIID in the speckles became more evident (Figure 3). Localization data of RNAP II and TFIID in the IGCs of mouse embryos are in accordance with the results that came from somatic cell studies [58, 59] including IGC proteome analysis [60].


Nuclear distribution of RNA polymerase II and mRNA processing machinery in early mammalian embryos.

Bogolyubova IO, Bogolyubov DS - Biomed Res Int (2014)

Double immunolocalization of SC35 (a), (b) and transcription factor TFIID (a′), (b′) in mouse embryos. TFIID is revealed in the nuclei at all studied stages. Colocalization of SC35 and TFIID is intensified during ZGA. Bar is 10 μm, according to [24], open access.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4020508&req=5

fig3: Double immunolocalization of SC35 (a), (b) and transcription factor TFIID (a′), (b′) in mouse embryos. TFIID is revealed in the nuclei at all studied stages. Colocalization of SC35 and TFIID is intensified during ZGA. Bar is 10 μm, according to [24], open access.
Mentions: In mouse embryo pronuclei, basal transcription factor TFIID has been revealed before the onset of ZGA in association with nuclear speckles already at the zygote stage [24]. During realization of ZGA, the presence of TFIID in the speckles became more evident (Figure 3). Localization data of RNAP II and TFIID in the IGCs of mouse embryos are in accordance with the results that came from somatic cell studies [58, 59] including IGC proteome analysis [60].

Bottom Line: Spatial distribution of components of nuclear metabolism provides a significant impact on regulation of the processes of gene expression.However, the period of cleavage is characterized by the most drastic and dynamic nuclear reorganizations accompanying zygotic gene activation.In this minireview, we try to summarize the results of studies concerning distribution of major factors involved in RNA polymerase II-dependent transcription, pre-mRNA splicing mRNA export that have been carried out on early embryos of mammals.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cell Morphology, Institute of Cytology RAS, 4 Tikhoretsky Avenue, St. Petersburg 194064, Russia.

ABSTRACT
Spatial distribution of components of nuclear metabolism provides a significant impact on regulation of the processes of gene expression. While distribution of the key nuclear antigens and their association with the defined nuclear domains were thoroughly traced in mammalian somatic cells, similar data for the preimplantation embryos are scanty and fragmental. However, the period of cleavage is characterized by the most drastic and dynamic nuclear reorganizations accompanying zygotic gene activation. In this minireview, we try to summarize the results of studies concerning distribution of major factors involved in RNA polymerase II-dependent transcription, pre-mRNA splicing mRNA export that have been carried out on early embryos of mammals.

Show MeSH