Limits...
Impaired hematopoiesis and delayed thrombopoietic recovery following sublethal irradiation in SRC‑3 knockout mice.

Jin J, Wang Y, Wang J, Xu Y, Chen SL, Wang JP, Su YP - Mol Med Rep (2014)

Bottom Line: Furthermore, peripheral blood cell counts, BM cellularity and colony-forming unit (CFU) assays were performed following irradiation.The results showed that peripheral blood cells were significantly lower in number and recovered less rapidly in irradiated SRC-3-/‑ mice as compared with control animals.In conclusion, the present study demonstrated that the hematopoietic ability in SRC-3 knockout mice is severely impaired following a sublethal dose of irradiation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, P.R. China.

ABSTRACT
The objective of the present study was to investigate the role of the steroid receptor coactivator-3 (SRC-3) in hematopoiesis of mouse bone marrow (BM) following total body irradiation (TBI). SRC-3-/‑ mice and wild-type (WT) mice were exposed to 4.5 Gy γ rays. Immunoblotting analysis revealed that the SRC-3 protein (p160) levels in normal BM-nucleated cells in WT were higher than in SRC-3-/‑ mice. Furthermore, peripheral blood cell counts, BM cellularity and colony-forming unit (CFU) assays were performed following irradiation. The results showed that peripheral blood cells were significantly lower in number and recovered less rapidly in irradiated SRC-3-/‑ mice as compared with control animals. BM-nucleated cell and CFU counts were significantly decreased in SRC-3-/‑ mice on the 7th and 14th day. Of note, the recovery of platelet (PLT) and megakaryocytic lineage were more depressed than the granulocytic and erythroid lineage in SRC-3-/‑ mice. In conclusion, the present study demonstrated that the hematopoietic ability in SRC-3 knockout mice is severely impaired following a sublethal dose of irradiation.

Show MeSH

Related in: MedlinePlus

The cellularity of BM-nucleated cells in WT and SRC-3−/− mice on the 7th and 14th day after TBI shown as mean ± standard deviation (n =3). The nucleated cell numbers in SRC-3−/− mice were significantly lower than in WT mice on days 7 and 14. *P<0.05, **P<0.01. BM, bone marrow; WT, wild-type; SRC-3, steroid receptor coactivator-3; TBI, total body irradiation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4020484&req=5

f3-mmr-09-05-1629: The cellularity of BM-nucleated cells in WT and SRC-3−/− mice on the 7th and 14th day after TBI shown as mean ± standard deviation (n =3). The nucleated cell numbers in SRC-3−/− mice were significantly lower than in WT mice on days 7 and 14. *P<0.05, **P<0.01. BM, bone marrow; WT, wild-type; SRC-3, steroid receptor coactivator-3; TBI, total body irradiation.

Mentions: To determine whether the decreased peripheral blood cell counts changed with the reduced cellularity of BM following TBI, the BM-nucleated cells were counted in WT and SRC-3−/− mice on the 7th and 14th day following irradiation. The BM cellularity was reduced in the WT and SRC-3−/− mice. However, the number of BM-nucleated cells in SRC-3−/− mice was significantly lower on the 7th and 14th day following irradiation (P<0.05) (Fig. 3). Histological examination also showed a greater reduction in the number of hematopoietic cells, particularly of megakaryocytes in the BM of SRC-3−/− mice (Fig. 4).


Impaired hematopoiesis and delayed thrombopoietic recovery following sublethal irradiation in SRC‑3 knockout mice.

Jin J, Wang Y, Wang J, Xu Y, Chen SL, Wang JP, Su YP - Mol Med Rep (2014)

The cellularity of BM-nucleated cells in WT and SRC-3−/− mice on the 7th and 14th day after TBI shown as mean ± standard deviation (n =3). The nucleated cell numbers in SRC-3−/− mice were significantly lower than in WT mice on days 7 and 14. *P<0.05, **P<0.01. BM, bone marrow; WT, wild-type; SRC-3, steroid receptor coactivator-3; TBI, total body irradiation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4020484&req=5

f3-mmr-09-05-1629: The cellularity of BM-nucleated cells in WT and SRC-3−/− mice on the 7th and 14th day after TBI shown as mean ± standard deviation (n =3). The nucleated cell numbers in SRC-3−/− mice were significantly lower than in WT mice on days 7 and 14. *P<0.05, **P<0.01. BM, bone marrow; WT, wild-type; SRC-3, steroid receptor coactivator-3; TBI, total body irradiation.
Mentions: To determine whether the decreased peripheral blood cell counts changed with the reduced cellularity of BM following TBI, the BM-nucleated cells were counted in WT and SRC-3−/− mice on the 7th and 14th day following irradiation. The BM cellularity was reduced in the WT and SRC-3−/− mice. However, the number of BM-nucleated cells in SRC-3−/− mice was significantly lower on the 7th and 14th day following irradiation (P<0.05) (Fig. 3). Histological examination also showed a greater reduction in the number of hematopoietic cells, particularly of megakaryocytes in the BM of SRC-3−/− mice (Fig. 4).

Bottom Line: Furthermore, peripheral blood cell counts, BM cellularity and colony-forming unit (CFU) assays were performed following irradiation.The results showed that peripheral blood cells were significantly lower in number and recovered less rapidly in irradiated SRC-3-/‑ mice as compared with control animals.In conclusion, the present study demonstrated that the hematopoietic ability in SRC-3 knockout mice is severely impaired following a sublethal dose of irradiation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, P.R. China.

ABSTRACT
The objective of the present study was to investigate the role of the steroid receptor coactivator-3 (SRC-3) in hematopoiesis of mouse bone marrow (BM) following total body irradiation (TBI). SRC-3-/‑ mice and wild-type (WT) mice were exposed to 4.5 Gy γ rays. Immunoblotting analysis revealed that the SRC-3 protein (p160) levels in normal BM-nucleated cells in WT were higher than in SRC-3-/‑ mice. Furthermore, peripheral blood cell counts, BM cellularity and colony-forming unit (CFU) assays were performed following irradiation. The results showed that peripheral blood cells were significantly lower in number and recovered less rapidly in irradiated SRC-3-/‑ mice as compared with control animals. BM-nucleated cell and CFU counts were significantly decreased in SRC-3-/‑ mice on the 7th and 14th day. Of note, the recovery of platelet (PLT) and megakaryocytic lineage were more depressed than the granulocytic and erythroid lineage in SRC-3-/‑ mice. In conclusion, the present study demonstrated that the hematopoietic ability in SRC-3 knockout mice is severely impaired following a sublethal dose of irradiation.

Show MeSH
Related in: MedlinePlus