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The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA.

Liu L, Wang Y, Yu Q - Int. J. Mol. Med. (2014)

Bottom Line: The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression.Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS).These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

View Article: PubMed Central - PubMed

Affiliation: College of Basic Medicine, Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.

ABSTRACT
The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uterus of mice on day 5 of pregnancy (D5) and in pseudopregnant mice was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis. A functional analysis of these genes was also performed by the intrauterine injection with the PI3K inhibitor, LY294002, on day 2 of pregnancy (D2). The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression. The expression levels of the above genes in the pseudopregnant group and in the group injected with the PI3K/Akt inhibitor, LY294002, were markedly lower than those in the pregnant group. Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS). These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

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Immunohistochemical analysis of the expression of PI3K, Akt, p-Akt, PTEN and RhoA at the implantation site in the group injected with the PI3K inhibitor in the uterus of pregnancy mice on day 5. Yellow-brown color represents positive staining. (A) Representative image of PI3K; (B) representative image of Akt; (C) representative image of p-Akt; (D) representative image of PTEN; (E) representative image of RhoA. The experiment was performed 3 times. Three mice were used in each experiment. Le, luminal epithelium; ge, glandular epithelium; s, stromal cells; bar, 125 μm.
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f8-ijmm-33-05-1089: Immunohistochemical analysis of the expression of PI3K, Akt, p-Akt, PTEN and RhoA at the implantation site in the group injected with the PI3K inhibitor in the uterus of pregnancy mice on day 5. Yellow-brown color represents positive staining. (A) Representative image of PI3K; (B) representative image of Akt; (C) representative image of p-Akt; (D) representative image of PTEN; (E) representative image of RhoA. The experiment was performed 3 times. Three mice were used in each experiment. Le, luminal epithelium; ge, glandular epithelium; s, stromal cells; bar, 125 μm.

Mentions: The expression of PI3K, Akt and p-Akt was significantly decreased in the group injected with the inhibitor compared with that in the non-injected control group. PTEN expression in the inhibitor group was significantly increased (Fig. 8) following the intrauterine injection of the PI3K inhibitor, LY294002. The expression of RhoA was decreased. In addition, the locations of the expression of the above genes had not changed, and the genes were mainly expressed in the stromal cells.


The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA.

Liu L, Wang Y, Yu Q - Int. J. Mol. Med. (2014)

Immunohistochemical analysis of the expression of PI3K, Akt, p-Akt, PTEN and RhoA at the implantation site in the group injected with the PI3K inhibitor in the uterus of pregnancy mice on day 5. Yellow-brown color represents positive staining. (A) Representative image of PI3K; (B) representative image of Akt; (C) representative image of p-Akt; (D) representative image of PTEN; (E) representative image of RhoA. The experiment was performed 3 times. Three mice were used in each experiment. Le, luminal epithelium; ge, glandular epithelium; s, stromal cells; bar, 125 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4020477&req=5

f8-ijmm-33-05-1089: Immunohistochemical analysis of the expression of PI3K, Akt, p-Akt, PTEN and RhoA at the implantation site in the group injected with the PI3K inhibitor in the uterus of pregnancy mice on day 5. Yellow-brown color represents positive staining. (A) Representative image of PI3K; (B) representative image of Akt; (C) representative image of p-Akt; (D) representative image of PTEN; (E) representative image of RhoA. The experiment was performed 3 times. Three mice were used in each experiment. Le, luminal epithelium; ge, glandular epithelium; s, stromal cells; bar, 125 μm.
Mentions: The expression of PI3K, Akt and p-Akt was significantly decreased in the group injected with the inhibitor compared with that in the non-injected control group. PTEN expression in the inhibitor group was significantly increased (Fig. 8) following the intrauterine injection of the PI3K inhibitor, LY294002. The expression of RhoA was decreased. In addition, the locations of the expression of the above genes had not changed, and the genes were mainly expressed in the stromal cells.

Bottom Line: The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression.Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS).These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

View Article: PubMed Central - PubMed

Affiliation: College of Basic Medicine, Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.

ABSTRACT
The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uterus of mice on day 5 of pregnancy (D5) and in pseudopregnant mice was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis. A functional analysis of these genes was also performed by the intrauterine injection with the PI3K inhibitor, LY294002, on day 2 of pregnancy (D2). The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression. The expression levels of the above genes in the pseudopregnant group and in the group injected with the PI3K/Akt inhibitor, LY294002, were markedly lower than those in the pregnant group. Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS). These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

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