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The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA.

Liu L, Wang Y, Yu Q - Int. J. Mol. Med. (2014)

Bottom Line: The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression.Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS).These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

View Article: PubMed Central - PubMed

Affiliation: College of Basic Medicine, Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.

ABSTRACT
The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uterus of mice on day 5 of pregnancy (D5) and in pseudopregnant mice was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis. A functional analysis of these genes was also performed by the intrauterine injection with the PI3K inhibitor, LY294002, on day 2 of pregnancy (D2). The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression. The expression levels of the above genes in the pseudopregnant group and in the group injected with the PI3K/Akt inhibitor, LY294002, were markedly lower than those in the pregnant group. Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS). These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

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Western blot analysis of the expression of PI3K, Akt, p-Akt, PTEN and RhoA in the inhibitor group injected with LY294002 (L) and the control group (Con; not injected). β-actin was used as the internal control. (A) Western blot analysis electrophoresis graph. (B) Relative densitometric analysis of these proteins between the inhibitor group and the control group. The experiment was performed 3 times. Three mice were used in each experiment. *P<0.05 indicates statistical significance between the inhibitor group and the control group at pregnancy day 5.
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f7-ijmm-33-05-1089: Western blot analysis of the expression of PI3K, Akt, p-Akt, PTEN and RhoA in the inhibitor group injected with LY294002 (L) and the control group (Con; not injected). β-actin was used as the internal control. (A) Western blot analysis electrophoresis graph. (B) Relative densitometric analysis of these proteins between the inhibitor group and the control group. The experiment was performed 3 times. Three mice were used in each experiment. *P<0.05 indicates statistical significance between the inhibitor group and the control group at pregnancy day 5.

Mentions: The expression of Akt showed no significant alteration; the expression of PI3K and p-Akt was decreased in the group injected with the PI3K inhibitor, LY294002, compared with the control group. The expression of PTEN was significantly increased, and RhoA expression was significantly decreased (Fig. 7) following the intrauterine injection of the PI3K inhibitor, LY294002.


The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA.

Liu L, Wang Y, Yu Q - Int. J. Mol. Med. (2014)

Western blot analysis of the expression of PI3K, Akt, p-Akt, PTEN and RhoA in the inhibitor group injected with LY294002 (L) and the control group (Con; not injected). β-actin was used as the internal control. (A) Western blot analysis electrophoresis graph. (B) Relative densitometric analysis of these proteins between the inhibitor group and the control group. The experiment was performed 3 times. Three mice were used in each experiment. *P<0.05 indicates statistical significance between the inhibitor group and the control group at pregnancy day 5.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4020477&req=5

f7-ijmm-33-05-1089: Western blot analysis of the expression of PI3K, Akt, p-Akt, PTEN and RhoA in the inhibitor group injected with LY294002 (L) and the control group (Con; not injected). β-actin was used as the internal control. (A) Western blot analysis electrophoresis graph. (B) Relative densitometric analysis of these proteins between the inhibitor group and the control group. The experiment was performed 3 times. Three mice were used in each experiment. *P<0.05 indicates statistical significance between the inhibitor group and the control group at pregnancy day 5.
Mentions: The expression of Akt showed no significant alteration; the expression of PI3K and p-Akt was decreased in the group injected with the PI3K inhibitor, LY294002, compared with the control group. The expression of PTEN was significantly increased, and RhoA expression was significantly decreased (Fig. 7) following the intrauterine injection of the PI3K inhibitor, LY294002.

Bottom Line: The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression.Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS).These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

View Article: PubMed Central - PubMed

Affiliation: College of Basic Medicine, Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.

ABSTRACT
The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uterus of mice on day 5 of pregnancy (D5) and in pseudopregnant mice was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis. A functional analysis of these genes was also performed by the intrauterine injection with the PI3K inhibitor, LY294002, on day 2 of pregnancy (D2). The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression. The expression levels of the above genes in the pseudopregnant group and in the group injected with the PI3K/Akt inhibitor, LY294002, were markedly lower than those in the pregnant group. Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS). These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

Show MeSH