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The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA.

Liu L, Wang Y, Yu Q - Int. J. Mol. Med. (2014)

Bottom Line: The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression.Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS).These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

View Article: PubMed Central - PubMed

Affiliation: College of Basic Medicine, Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.

ABSTRACT
The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uterus of mice on day 5 of pregnancy (D5) and in pseudopregnant mice was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis. A functional analysis of these genes was also performed by the intrauterine injection with the PI3K inhibitor, LY294002, on day 2 of pregnancy (D2). The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression. The expression levels of the above genes in the pseudopregnant group and in the group injected with the PI3K/Akt inhibitor, LY294002, were markedly lower than those in the pregnant group. Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS). These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

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qRT-PCR of the mRNA expression of PI3K, Akt, PTEN and RhoA at the implantation site in the inhibitor group injected with LY294002 (L) and the control group (Con; not injected). β-actin was used as the internal control. The experiment was performed 3 times. Three mice were used in each experiment. *P<0.05 and **P<0.01 indicates statistical significance between the pregnant group and the inhibitor group.
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f6-ijmm-33-05-1089: qRT-PCR of the mRNA expression of PI3K, Akt, PTEN and RhoA at the implantation site in the inhibitor group injected with LY294002 (L) and the control group (Con; not injected). β-actin was used as the internal control. The experiment was performed 3 times. Three mice were used in each experiment. *P<0.05 and **P<0.01 indicates statistical significance between the pregnant group and the inhibitor group.

Mentions: The expression of PI3K and Akt was significantly decreased (P<0.05) when comparing the inhibitor group with the control group (Fig. 6) following the intrauterine injection of the PI3K inhibitor, LY294002, and PTEN expression was significantly increased (P<0.01); RhoA expression was significantly decreased (P<0.05).


The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA.

Liu L, Wang Y, Yu Q - Int. J. Mol. Med. (2014)

qRT-PCR of the mRNA expression of PI3K, Akt, PTEN and RhoA at the implantation site in the inhibitor group injected with LY294002 (L) and the control group (Con; not injected). β-actin was used as the internal control. The experiment was performed 3 times. Three mice were used in each experiment. *P<0.05 and **P<0.01 indicates statistical significance between the pregnant group and the inhibitor group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4020477&req=5

f6-ijmm-33-05-1089: qRT-PCR of the mRNA expression of PI3K, Akt, PTEN and RhoA at the implantation site in the inhibitor group injected with LY294002 (L) and the control group (Con; not injected). β-actin was used as the internal control. The experiment was performed 3 times. Three mice were used in each experiment. *P<0.05 and **P<0.01 indicates statistical significance between the pregnant group and the inhibitor group.
Mentions: The expression of PI3K and Akt was significantly decreased (P<0.05) when comparing the inhibitor group with the control group (Fig. 6) following the intrauterine injection of the PI3K inhibitor, LY294002, and PTEN expression was significantly increased (P<0.01); RhoA expression was significantly decreased (P<0.05).

Bottom Line: The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression.Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS).These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

View Article: PubMed Central - PubMed

Affiliation: College of Basic Medicine, Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.

ABSTRACT
The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uterus of mice on day 5 of pregnancy (D5) and in pseudopregnant mice was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis. A functional analysis of these genes was also performed by the intrauterine injection with the PI3K inhibitor, LY294002, on day 2 of pregnancy (D2). The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression. The expression levels of the above genes in the pseudopregnant group and in the group injected with the PI3K/Akt inhibitor, LY294002, were markedly lower than those in the pregnant group. Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS). These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

Show MeSH