Limits...
The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA.

Liu L, Wang Y, Yu Q - Int. J. Mol. Med. (2014)

Bottom Line: The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression.Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS).These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

View Article: PubMed Central - PubMed

Affiliation: College of Basic Medicine, Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.

ABSTRACT
The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uterus of mice on day 5 of pregnancy (D5) and in pseudopregnant mice was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis. A functional analysis of these genes was also performed by the intrauterine injection with the PI3K inhibitor, LY294002, on day 2 of pregnancy (D2). The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression. The expression levels of the above genes in the pseudopregnant group and in the group injected with the PI3K/Akt inhibitor, LY294002, were markedly lower than those in the pregnant group. Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS). These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

Show MeSH
Immunohistochemical analysis of the expression of PI3K/Akt signaling pathway-related genes and RhoA at the implantation site and the inter-implantation site on day 5 of pregnant (D5) mice. The experiment was performed 3 times. Three mice were used in each experiment. (A, C, E, G and I) Representative images of implantation site; (B, D, F, H and J) representative images of inter-implantation site; (K) negative control. (A and B) PI3K, (C and D) Akt, (E and F) p-Akt, (G and H) PTEN, (I and J) RhoA. Yellow-brown color represents positive staining. IS, implantation site; inter-IS, inter-implantation site; le, luminal epithelium; ge, glandular epithelium; s, stromal cells; bar, 125 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4020477&req=5

f3-ijmm-33-05-1089: Immunohistochemical analysis of the expression of PI3K/Akt signaling pathway-related genes and RhoA at the implantation site and the inter-implantation site on day 5 of pregnant (D5) mice. The experiment was performed 3 times. Three mice were used in each experiment. (A, C, E, G and I) Representative images of implantation site; (B, D, F, H and J) representative images of inter-implantation site; (K) negative control. (A and B) PI3K, (C and D) Akt, (E and F) p-Akt, (G and H) PTEN, (I and J) RhoA. Yellow-brown color represents positive staining. IS, implantation site; inter-IS, inter-implantation site; le, luminal epithelium; ge, glandular epithelium; s, stromal cells; bar, 125 μm.

Mentions: Immunohistochemistry (Fig. 3) revealed that the gene expression of PI3K, Akt, p-Akt and RhoA at the implantation site in the uterus of pregnant mice on day 5 was significantly higher than that at the inter-implantation site; however, the opposite effect was observed for PTEN. In the endometrium of pregnant mice on day 5, PI3K expression was strongly positive in the glandular epithelium and stromal cells at the implantation site, while at the inter-implantation site, PI3K protein was significantly expressed in the glandular epithelium and weakly expressed in the stromal cells. Akt protein was significantly expressed in the stromal cells and luminal epithelium of the uterine implantation site, while it was weakly expressed in the luminal epithelium of the inter-implantation site. p-Akt protein was strongly expressed in the stromal cells of the uterine implantation site, and weakly expressed in the luminal epithelium of the uterine inter-implantation site. PTEN protein was expressed in the stromal cells of the implantation site in the endometrium of the pregnant mice on day 5, and weakly expressed in the stromal cells and luminal epithelium of the mouse endometrium inter-implantation site. RhoA was highly expressed in the stromal cells and glandular epithelium at the implantation site in the endometrium of mice, and significantly expressed in the luminal epithelium at the inter-implantation.


The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA.

Liu L, Wang Y, Yu Q - Int. J. Mol. Med. (2014)

Immunohistochemical analysis of the expression of PI3K/Akt signaling pathway-related genes and RhoA at the implantation site and the inter-implantation site on day 5 of pregnant (D5) mice. The experiment was performed 3 times. Three mice were used in each experiment. (A, C, E, G and I) Representative images of implantation site; (B, D, F, H and J) representative images of inter-implantation site; (K) negative control. (A and B) PI3K, (C and D) Akt, (E and F) p-Akt, (G and H) PTEN, (I and J) RhoA. Yellow-brown color represents positive staining. IS, implantation site; inter-IS, inter-implantation site; le, luminal epithelium; ge, glandular epithelium; s, stromal cells; bar, 125 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4020477&req=5

f3-ijmm-33-05-1089: Immunohistochemical analysis of the expression of PI3K/Akt signaling pathway-related genes and RhoA at the implantation site and the inter-implantation site on day 5 of pregnant (D5) mice. The experiment was performed 3 times. Three mice were used in each experiment. (A, C, E, G and I) Representative images of implantation site; (B, D, F, H and J) representative images of inter-implantation site; (K) negative control. (A and B) PI3K, (C and D) Akt, (E and F) p-Akt, (G and H) PTEN, (I and J) RhoA. Yellow-brown color represents positive staining. IS, implantation site; inter-IS, inter-implantation site; le, luminal epithelium; ge, glandular epithelium; s, stromal cells; bar, 125 μm.
Mentions: Immunohistochemistry (Fig. 3) revealed that the gene expression of PI3K, Akt, p-Akt and RhoA at the implantation site in the uterus of pregnant mice on day 5 was significantly higher than that at the inter-implantation site; however, the opposite effect was observed for PTEN. In the endometrium of pregnant mice on day 5, PI3K expression was strongly positive in the glandular epithelium and stromal cells at the implantation site, while at the inter-implantation site, PI3K protein was significantly expressed in the glandular epithelium and weakly expressed in the stromal cells. Akt protein was significantly expressed in the stromal cells and luminal epithelium of the uterine implantation site, while it was weakly expressed in the luminal epithelium of the inter-implantation site. p-Akt protein was strongly expressed in the stromal cells of the uterine implantation site, and weakly expressed in the luminal epithelium of the uterine inter-implantation site. PTEN protein was expressed in the stromal cells of the implantation site in the endometrium of the pregnant mice on day 5, and weakly expressed in the stromal cells and luminal epithelium of the mouse endometrium inter-implantation site. RhoA was highly expressed in the stromal cells and glandular epithelium at the implantation site in the endometrium of mice, and significantly expressed in the luminal epithelium at the inter-implantation.

Bottom Line: The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression.Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS).These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

View Article: PubMed Central - PubMed

Affiliation: College of Basic Medicine, Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.

ABSTRACT
The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uterus of mice on day 5 of pregnancy (D5) and in pseudopregnant mice was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis. A functional analysis of these genes was also performed by the intrauterine injection with the PI3K inhibitor, LY294002, on day 2 of pregnancy (D2). The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression. The expression levels of the above genes in the pseudopregnant group and in the group injected with the PI3K/Akt inhibitor, LY294002, were markedly lower than those in the pregnant group. Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS). These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

Show MeSH