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The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA.

Liu L, Wang Y, Yu Q - Int. J. Mol. Med. (2014)

Bottom Line: The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression.Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS).These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

View Article: PubMed Central - PubMed

Affiliation: College of Basic Medicine, Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.

ABSTRACT
The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uterus of mice on day 5 of pregnancy (D5) and in pseudopregnant mice was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis. A functional analysis of these genes was also performed by the intrauterine injection with the PI3K inhibitor, LY294002, on day 2 of pregnancy (D2). The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression. The expression levels of the above genes in the pseudopregnant group and in the group injected with the PI3K/Akt inhibitor, LY294002, were markedly lower than those in the pregnant group. Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS). These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

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Real-time polymerase chain reaction (PCR) of the mRNA expression levels of PI3K, Akt, PTEN and RhoA at the implantation site and the inter-implantation site in the endometrium. β-actin was used as an internal control. The experiment was performed 3 times. Three mice were used in each experiment. The analysis was performed on day 5. **P<0.01 indicates statistical significance between the implantation site and inter-implantation site in the endometrium. IS, implantation site; inter-IS, inter-implantation site.
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f1-ijmm-33-05-1089: Real-time polymerase chain reaction (PCR) of the mRNA expression levels of PI3K, Akt, PTEN and RhoA at the implantation site and the inter-implantation site in the endometrium. β-actin was used as an internal control. The experiment was performed 3 times. Three mice were used in each experiment. The analysis was performed on day 5. **P<0.01 indicates statistical significance between the implantation site and inter-implantation site in the endometrium. IS, implantation site; inter-IS, inter-implantation site.

Mentions: qRT-PCR (Fig. 1) revealed that the mRNA expression levels of PI3K, Akt and RhoA at the implantation site in the endometrium on day 5 of pregnancy were significantly higher than those at the inter-implantation site (P<0.01). However, the mRNA expression level of PTEN at the implantation site was significantly lower than that at the inter-implantation site (P<0.01).


The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA.

Liu L, Wang Y, Yu Q - Int. J. Mol. Med. (2014)

Real-time polymerase chain reaction (PCR) of the mRNA expression levels of PI3K, Akt, PTEN and RhoA at the implantation site and the inter-implantation site in the endometrium. β-actin was used as an internal control. The experiment was performed 3 times. Three mice were used in each experiment. The analysis was performed on day 5. **P<0.01 indicates statistical significance between the implantation site and inter-implantation site in the endometrium. IS, implantation site; inter-IS, inter-implantation site.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4020477&req=5

f1-ijmm-33-05-1089: Real-time polymerase chain reaction (PCR) of the mRNA expression levels of PI3K, Akt, PTEN and RhoA at the implantation site and the inter-implantation site in the endometrium. β-actin was used as an internal control. The experiment was performed 3 times. Three mice were used in each experiment. The analysis was performed on day 5. **P<0.01 indicates statistical significance between the implantation site and inter-implantation site in the endometrium. IS, implantation site; inter-IS, inter-implantation site.
Mentions: qRT-PCR (Fig. 1) revealed that the mRNA expression levels of PI3K, Akt and RhoA at the implantation site in the endometrium on day 5 of pregnancy were significantly higher than those at the inter-implantation site (P<0.01). However, the mRNA expression level of PTEN at the implantation site was significantly lower than that at the inter-implantation site (P<0.01).

Bottom Line: The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression.Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS).These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

View Article: PubMed Central - PubMed

Affiliation: College of Basic Medicine, Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.

ABSTRACT
The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uterus of mice on day 5 of pregnancy (D5) and in pseudopregnant mice was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis. A functional analysis of these genes was also performed by the intrauterine injection with the PI3K inhibitor, LY294002, on day 2 of pregnancy (D2). The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression. The expression levels of the above genes in the pseudopregnant group and in the group injected with the PI3K/Akt inhibitor, LY294002, were markedly lower than those in the pregnant group. Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS). These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.

Show MeSH