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KLF8 knockdown suppresses proliferation and invasion in human osteosarcoma cells.

Lin F, Shen Z, Tang LN, Zheng SE, Sun YJ, Min DL, Yao Y - Mol Med Rep (2014)

Bottom Line: Krüppel-like factor 8 (KLF8) is a transcription factor that is important in the regulation of the cell cycle and has a critical role in oncogenic transformation and epithelial to mesenchymal transition (EMT).EMT is a key process in tumor metastasis.KLF8 gene expression was knocked down in vitro using a lentivirus-mediated small interfering (si)RNA method.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Medicine, Soochow University, Suzhou, Jiangsu 215006, P.R. China.

ABSTRACT
Krüppel-like factor 8 (KLF8) is a transcription factor that is important in the regulation of the cell cycle and has a critical role in oncogenic transformation and epithelial to mesenchymal transition (EMT). EMT is a key process in tumor metastasis. Although overexpression of KLF8 has been observed in a variety of human tumor types, the role of KLF8 in human osteosarcoma is yet to be elucidated. The present study aimed to investigate the biological impact of KLF8 on Saos-2 osteosarcoma cells. KLF8 gene expression was knocked down in vitro using a lentivirus-mediated small interfering (si)RNA method. Cell proliferation and cell cycle distribution were evaluated using 3-(4,5)-dimethylthiahiazo(-z-yl)-3,5-di-phenytetrazoliumromide and colony formation assays, and flow cytometry, respectively. Cell invasion was analyzed using a Transwell® invasion assay. Knockdown of KLF8 was found to significantly inhibit proliferation and invasion in osteosarcoma cells. These data suggest that KLF8 may exhibit an important role in osteosarcoma tumorigenesis and that KLF8 may be a potential therapeutic target for the treatment of osteosarcoma.

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Related in: MedlinePlus

Effect of KLF8 siRNA-expressing lentivirus treatment on Saos-2 cell invasion. (A) Crystal violet staining of cells which invaded through the polycarbonate membranes in the invasion assay. (B) The average number of migrated cells observed in each group. Saos-2 cell migration was found to be significantly decreased in the KLF8 siRNA treatment group. (C) Quantitative measurement of cell invasion using a spectrophotometer. Saos-2 cell invasion was found to be significantly reduced in the KLF8 siRNA treatment group. Values are presented as the mean ± standard deviation of three independent experiments. siRNA, small interfering RNA; KLF8, Krüppel-like factor 8.
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f4-mmr-09-05-1613: Effect of KLF8 siRNA-expressing lentivirus treatment on Saos-2 cell invasion. (A) Crystal violet staining of cells which invaded through the polycarbonate membranes in the invasion assay. (B) The average number of migrated cells observed in each group. Saos-2 cell migration was found to be significantly decreased in the KLF8 siRNA treatment group. (C) Quantitative measurement of cell invasion using a spectrophotometer. Saos-2 cell invasion was found to be significantly reduced in the KLF8 siRNA treatment group. Values are presented as the mean ± standard deviation of three independent experiments. siRNA, small interfering RNA; KLF8, Krüppel-like factor 8.

Mentions: To investigate the effect of KLF8 knockdown on osteosarcoma cell invasion, Saos-2 cells were analyzed using a Transwell assay and crystal violet staining. As shown in Fig. 4, the invasive potential of Saos-2 cells was significantly reduced with lentivirus-mediated KLF8 siRNA treatment compared with the control group (P<0.05), indicating that KLF8 may have a role in promoting osteosarcoma cell invasion.


KLF8 knockdown suppresses proliferation and invasion in human osteosarcoma cells.

Lin F, Shen Z, Tang LN, Zheng SE, Sun YJ, Min DL, Yao Y - Mol Med Rep (2014)

Effect of KLF8 siRNA-expressing lentivirus treatment on Saos-2 cell invasion. (A) Crystal violet staining of cells which invaded through the polycarbonate membranes in the invasion assay. (B) The average number of migrated cells observed in each group. Saos-2 cell migration was found to be significantly decreased in the KLF8 siRNA treatment group. (C) Quantitative measurement of cell invasion using a spectrophotometer. Saos-2 cell invasion was found to be significantly reduced in the KLF8 siRNA treatment group. Values are presented as the mean ± standard deviation of three independent experiments. siRNA, small interfering RNA; KLF8, Krüppel-like factor 8.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4020472&req=5

f4-mmr-09-05-1613: Effect of KLF8 siRNA-expressing lentivirus treatment on Saos-2 cell invasion. (A) Crystal violet staining of cells which invaded through the polycarbonate membranes in the invasion assay. (B) The average number of migrated cells observed in each group. Saos-2 cell migration was found to be significantly decreased in the KLF8 siRNA treatment group. (C) Quantitative measurement of cell invasion using a spectrophotometer. Saos-2 cell invasion was found to be significantly reduced in the KLF8 siRNA treatment group. Values are presented as the mean ± standard deviation of three independent experiments. siRNA, small interfering RNA; KLF8, Krüppel-like factor 8.
Mentions: To investigate the effect of KLF8 knockdown on osteosarcoma cell invasion, Saos-2 cells were analyzed using a Transwell assay and crystal violet staining. As shown in Fig. 4, the invasive potential of Saos-2 cells was significantly reduced with lentivirus-mediated KLF8 siRNA treatment compared with the control group (P<0.05), indicating that KLF8 may have a role in promoting osteosarcoma cell invasion.

Bottom Line: Krüppel-like factor 8 (KLF8) is a transcription factor that is important in the regulation of the cell cycle and has a critical role in oncogenic transformation and epithelial to mesenchymal transition (EMT).EMT is a key process in tumor metastasis.KLF8 gene expression was knocked down in vitro using a lentivirus-mediated small interfering (si)RNA method.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Medicine, Soochow University, Suzhou, Jiangsu 215006, P.R. China.

ABSTRACT
Krüppel-like factor 8 (KLF8) is a transcription factor that is important in the regulation of the cell cycle and has a critical role in oncogenic transformation and epithelial to mesenchymal transition (EMT). EMT is a key process in tumor metastasis. Although overexpression of KLF8 has been observed in a variety of human tumor types, the role of KLF8 in human osteosarcoma is yet to be elucidated. The present study aimed to investigate the biological impact of KLF8 on Saos-2 osteosarcoma cells. KLF8 gene expression was knocked down in vitro using a lentivirus-mediated small interfering (si)RNA method. Cell proliferation and cell cycle distribution were evaluated using 3-(4,5)-dimethylthiahiazo(-z-yl)-3,5-di-phenytetrazoliumromide and colony formation assays, and flow cytometry, respectively. Cell invasion was analyzed using a Transwell® invasion assay. Knockdown of KLF8 was found to significantly inhibit proliferation and invasion in osteosarcoma cells. These data suggest that KLF8 may exhibit an important role in osteosarcoma tumorigenesis and that KLF8 may be a potential therapeutic target for the treatment of osteosarcoma.

Show MeSH
Related in: MedlinePlus