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Human periodontal ligament derived progenitor cells: effect of STRO-1 cell sorting and Wnt3a treatment on cell behavior.

Yan XZ, Both SK, Yang PS, Jansen JA, van den Beucken JJ, Yang F - Biomed Res Int (2014)

Bottom Line: STRO-1 positive hPDLCs were sorted and the sorted cells were expanded and compared with their unsorted parental cells.Expanded STRO-1-sorted hPDLCs showed no superiority compared to their unsorted parental cells.On the other hand, Wnt3a promotes the efficient hPDLC expansion and retains the self-renewal and osteogenic differentiation capacity.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology and Biomaterials, Radboud University Medical Center, 309 Dentistry, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands ; Department of Periodontology, Shandong University, Jinan, Shandong 250012, China.

ABSTRACT

Objectives: STRO-1 positive periodontal ligament cells (PDLCs) and unsorted PDLCs have demonstrated potential for periodontal regeneration, but the comparison between unsorted cells and the expanded STRO-1 sorted cells has never been reported. Additionally, Wnt3a is involved in cell proliferation thus may benefit in vitro PDLC expansion. The aim was to evaluate the effect of STRO-1 cell sorting and Wnt3a treatment on cell behavior of human PDLCs (hPDLCs).

Materials and methods: STRO-1 positive hPDLCs were sorted and the sorted cells were expanded and compared with their unsorted parental cells. Thereafter, hPDLCs were treated with or without Wnt3a and the cell proliferation, self-renewal, and osteogenic differentiation were evaluated.

Results: No differences were measured between the expanded STRO-1-sorted cells and unsorted parental cells in terms of proliferation, CFU, and mineralization capacity. Wnt3a enhanced the proliferation and self-renewal ability of hPDLCs significantly as displayed by higher DNA content values, a shorter cell population doubling time, and higher expression of the self-renewal gene Oct4. Moreover, Wnt3a promoted the expansion of hPDLCs for 5 passages without affecting cell proliferation, CFU, and osteogenic capacity.

Conclusions: Expanded STRO-1-sorted hPDLCs showed no superiority compared to their unsorted parental cells. On the other hand, Wnt3a promotes the efficient hPDLC expansion and retains the self-renewal and osteogenic differentiation capacity.

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Related in: MedlinePlus

Representative results showed Wnt3a promoted proliferation and self-renewal ability of hPDLCs. (a) Wnt3a-treated cells displayed a significant increase in DNA content compared to untreated cells cultured in proliferation medium. (b) Wnt3a-treated cells exhibited a higher expression of Oct4, Nanog, and Sox2 after 5 days of culture. *P < 0.05; error bars represent standard deviation.
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fig3: Representative results showed Wnt3a promoted proliferation and self-renewal ability of hPDLCs. (a) Wnt3a-treated cells displayed a significant increase in DNA content compared to untreated cells cultured in proliferation medium. (b) Wnt3a-treated cells exhibited a higher expression of Oct4, Nanog, and Sox2 after 5 days of culture. *P < 0.05; error bars represent standard deviation.

Mentions: The effect of Wnt3a on hPDLC proliferation was assessed by measuring cell DNA content and calculating cell doubling time during passaging. Wnt3a treated cells exhibited a significant increase (P < 0.05; around 50% increase for day 4 and day 6 in DNA content compared to the nontreated control group; Figure 3(a)). In addition, Wnt3a-treated cells displayed significantly shorter population doubling time when compared to untreated control cells, which were 25.46 ± 3.76 versus 30.73 ± 0.9 hours (P < 0.05).


Human periodontal ligament derived progenitor cells: effect of STRO-1 cell sorting and Wnt3a treatment on cell behavior.

Yan XZ, Both SK, Yang PS, Jansen JA, van den Beucken JJ, Yang F - Biomed Res Int (2014)

Representative results showed Wnt3a promoted proliferation and self-renewal ability of hPDLCs. (a) Wnt3a-treated cells displayed a significant increase in DNA content compared to untreated cells cultured in proliferation medium. (b) Wnt3a-treated cells exhibited a higher expression of Oct4, Nanog, and Sox2 after 5 days of culture. *P < 0.05; error bars represent standard deviation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4020471&req=5

fig3: Representative results showed Wnt3a promoted proliferation and self-renewal ability of hPDLCs. (a) Wnt3a-treated cells displayed a significant increase in DNA content compared to untreated cells cultured in proliferation medium. (b) Wnt3a-treated cells exhibited a higher expression of Oct4, Nanog, and Sox2 after 5 days of culture. *P < 0.05; error bars represent standard deviation.
Mentions: The effect of Wnt3a on hPDLC proliferation was assessed by measuring cell DNA content and calculating cell doubling time during passaging. Wnt3a treated cells exhibited a significant increase (P < 0.05; around 50% increase for day 4 and day 6 in DNA content compared to the nontreated control group; Figure 3(a)). In addition, Wnt3a-treated cells displayed significantly shorter population doubling time when compared to untreated control cells, which were 25.46 ± 3.76 versus 30.73 ± 0.9 hours (P < 0.05).

Bottom Line: STRO-1 positive hPDLCs were sorted and the sorted cells were expanded and compared with their unsorted parental cells.Expanded STRO-1-sorted hPDLCs showed no superiority compared to their unsorted parental cells.On the other hand, Wnt3a promotes the efficient hPDLC expansion and retains the self-renewal and osteogenic differentiation capacity.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology and Biomaterials, Radboud University Medical Center, 309 Dentistry, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands ; Department of Periodontology, Shandong University, Jinan, Shandong 250012, China.

ABSTRACT

Objectives: STRO-1 positive periodontal ligament cells (PDLCs) and unsorted PDLCs have demonstrated potential for periodontal regeneration, but the comparison between unsorted cells and the expanded STRO-1 sorted cells has never been reported. Additionally, Wnt3a is involved in cell proliferation thus may benefit in vitro PDLC expansion. The aim was to evaluate the effect of STRO-1 cell sorting and Wnt3a treatment on cell behavior of human PDLCs (hPDLCs).

Materials and methods: STRO-1 positive hPDLCs were sorted and the sorted cells were expanded and compared with their unsorted parental cells. Thereafter, hPDLCs were treated with or without Wnt3a and the cell proliferation, self-renewal, and osteogenic differentiation were evaluated.

Results: No differences were measured between the expanded STRO-1-sorted cells and unsorted parental cells in terms of proliferation, CFU, and mineralization capacity. Wnt3a enhanced the proliferation and self-renewal ability of hPDLCs significantly as displayed by higher DNA content values, a shorter cell population doubling time, and higher expression of the self-renewal gene Oct4. Moreover, Wnt3a promoted the expansion of hPDLCs for 5 passages without affecting cell proliferation, CFU, and osteogenic capacity.

Conclusions: Expanded STRO-1-sorted hPDLCs showed no superiority compared to their unsorted parental cells. On the other hand, Wnt3a promotes the efficient hPDLC expansion and retains the self-renewal and osteogenic differentiation capacity.

Show MeSH
Related in: MedlinePlus