Limits...
Human periodontal ligament derived progenitor cells: effect of STRO-1 cell sorting and Wnt3a treatment on cell behavior.

Yan XZ, Both SK, Yang PS, Jansen JA, van den Beucken JJ, Yang F - Biomed Res Int (2014)

Bottom Line: Additionally, Wnt3a is involved in cell proliferation thus may benefit in vitro PDLC expansion.STRO-1 positive hPDLCs were sorted and the sorted cells were expanded and compared with their unsorted parental cells.Expanded STRO-1-sorted hPDLCs showed no superiority compared to their unsorted parental cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology and Biomaterials, Radboud University Medical Center, 309 Dentistry, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands ; Department of Periodontology, Shandong University, Jinan, Shandong 250012, China.

ABSTRACT

Objectives: STRO-1 positive periodontal ligament cells (PDLCs) and unsorted PDLCs have demonstrated potential for periodontal regeneration, but the comparison between unsorted cells and the expanded STRO-1 sorted cells has never been reported. Additionally, Wnt3a is involved in cell proliferation thus may benefit in vitro PDLC expansion. The aim was to evaluate the effect of STRO-1 cell sorting and Wnt3a treatment on cell behavior of human PDLCs (hPDLCs).

Materials and methods: STRO-1 positive hPDLCs were sorted and the sorted cells were expanded and compared with their unsorted parental cells. Thereafter, hPDLCs were treated with or without Wnt3a and the cell proliferation, self-renewal, and osteogenic differentiation were evaluated.

Results: No differences were measured between the expanded STRO-1-sorted cells and unsorted parental cells in terms of proliferation, CFU, and mineralization capacity. Wnt3a enhanced the proliferation and self-renewal ability of hPDLCs significantly as displayed by higher DNA content values, a shorter cell population doubling time, and higher expression of the self-renewal gene Oct4. Moreover, Wnt3a promoted the expansion of hPDLCs for 5 passages without affecting cell proliferation, CFU, and osteogenic capacity.

Conclusions: Expanded STRO-1-sorted hPDLCs showed no superiority compared to their unsorted parental cells. On the other hand, Wnt3a promotes the efficient hPDLC expansion and retains the self-renewal and osteogenic differentiation capacity.

Show MeSH
Representative results of STRO-1 cell sorting. (a) The percentage of sorted STRO-1+ cells decreased to 2.3% after 4 passages of in vitro expansion. (b) No difference in proliferation was observed between the expanded STRO-1-sorted cells and the unsorted parental cells. (c) Expanded STRO-1-sorted cells displayed no difference compared to the unsorted parental cells in colony forming number. (d) No difference in calcium content was observed between expanded STRO-1-sorted cells and parental hPDLCs. Error bars represent standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4020471&req=5

fig2: Representative results of STRO-1 cell sorting. (a) The percentage of sorted STRO-1+ cells decreased to 2.3% after 4 passages of in vitro expansion. (b) No difference in proliferation was observed between the expanded STRO-1-sorted cells and the unsorted parental cells. (c) Expanded STRO-1-sorted cells displayed no difference compared to the unsorted parental cells in colony forming number. (d) No difference in calcium content was observed between expanded STRO-1-sorted cells and parental hPDLCs. Error bars represent standard deviation.

Mentions: The efficiency of FACS sorting was proved by fluorescent microscopy observation and flow cytometry. The STRO-1+ cells were expanded for 4 passages to achieve 10 × 106 cells, the same number as their unsorted parental cells. During this expansion, the percentage of STRO-1+ cells decreased significantly from 95.3% to 2.3% (Figure 2(a)). Additionally, after 4 passages of in vitro expansion, no significant difference was observed between the expanded STRO-1-sorted cells and unsorted parental cells in terms of DNA content, CFU number, and calcium content (Figures 2(b)–2(d)).


Human periodontal ligament derived progenitor cells: effect of STRO-1 cell sorting and Wnt3a treatment on cell behavior.

Yan XZ, Both SK, Yang PS, Jansen JA, van den Beucken JJ, Yang F - Biomed Res Int (2014)

Representative results of STRO-1 cell sorting. (a) The percentage of sorted STRO-1+ cells decreased to 2.3% after 4 passages of in vitro expansion. (b) No difference in proliferation was observed between the expanded STRO-1-sorted cells and the unsorted parental cells. (c) Expanded STRO-1-sorted cells displayed no difference compared to the unsorted parental cells in colony forming number. (d) No difference in calcium content was observed between expanded STRO-1-sorted cells and parental hPDLCs. Error bars represent standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4020471&req=5

fig2: Representative results of STRO-1 cell sorting. (a) The percentage of sorted STRO-1+ cells decreased to 2.3% after 4 passages of in vitro expansion. (b) No difference in proliferation was observed between the expanded STRO-1-sorted cells and the unsorted parental cells. (c) Expanded STRO-1-sorted cells displayed no difference compared to the unsorted parental cells in colony forming number. (d) No difference in calcium content was observed between expanded STRO-1-sorted cells and parental hPDLCs. Error bars represent standard deviation.
Mentions: The efficiency of FACS sorting was proved by fluorescent microscopy observation and flow cytometry. The STRO-1+ cells were expanded for 4 passages to achieve 10 × 106 cells, the same number as their unsorted parental cells. During this expansion, the percentage of STRO-1+ cells decreased significantly from 95.3% to 2.3% (Figure 2(a)). Additionally, after 4 passages of in vitro expansion, no significant difference was observed between the expanded STRO-1-sorted cells and unsorted parental cells in terms of DNA content, CFU number, and calcium content (Figures 2(b)–2(d)).

Bottom Line: Additionally, Wnt3a is involved in cell proliferation thus may benefit in vitro PDLC expansion.STRO-1 positive hPDLCs were sorted and the sorted cells were expanded and compared with their unsorted parental cells.Expanded STRO-1-sorted hPDLCs showed no superiority compared to their unsorted parental cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology and Biomaterials, Radboud University Medical Center, 309 Dentistry, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands ; Department of Periodontology, Shandong University, Jinan, Shandong 250012, China.

ABSTRACT

Objectives: STRO-1 positive periodontal ligament cells (PDLCs) and unsorted PDLCs have demonstrated potential for periodontal regeneration, but the comparison between unsorted cells and the expanded STRO-1 sorted cells has never been reported. Additionally, Wnt3a is involved in cell proliferation thus may benefit in vitro PDLC expansion. The aim was to evaluate the effect of STRO-1 cell sorting and Wnt3a treatment on cell behavior of human PDLCs (hPDLCs).

Materials and methods: STRO-1 positive hPDLCs were sorted and the sorted cells were expanded and compared with their unsorted parental cells. Thereafter, hPDLCs were treated with or without Wnt3a and the cell proliferation, self-renewal, and osteogenic differentiation were evaluated.

Results: No differences were measured between the expanded STRO-1-sorted cells and unsorted parental cells in terms of proliferation, CFU, and mineralization capacity. Wnt3a enhanced the proliferation and self-renewal ability of hPDLCs significantly as displayed by higher DNA content values, a shorter cell population doubling time, and higher expression of the self-renewal gene Oct4. Moreover, Wnt3a promoted the expansion of hPDLCs for 5 passages without affecting cell proliferation, CFU, and osteogenic capacity.

Conclusions: Expanded STRO-1-sorted hPDLCs showed no superiority compared to their unsorted parental cells. On the other hand, Wnt3a promotes the efficient hPDLC expansion and retains the self-renewal and osteogenic differentiation capacity.

Show MeSH