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Proanthocyanidin from grape seed extracts protects indomethacin-induced small intestinal mucosal injury.

Cheung DY, Kim JI, Park SH, Kim JK - Gastroenterol Res Pract (2014)

Bottom Line: The number of ulcer count was reduced to 0.1 ± 0.3 per rat in GSPEs treated group compared to 1.4 ± 0.5 per rat in indomethacin control group.Submucosal inflammatory cell infiltration was also reduced to 50% in GSPEs treated group.The tissue level of prostaglandin E2 was not affected by GSPEs treatment.

View Article: PubMed Central - PubMed

Affiliation: The Department of Internal Medicine, The Catholic University of Korea College of Medicine, St. Mary's Hospital, Yeongdeungpo-gu 63-ro 10, Seoul 150-713, Republic of Korea.

ABSTRACT
Proanthocyanidin (grape seed proanthocyanidin extracts, GSPEs) is an antioxidant and scavenges free radicals. Excessive oxidative stress and free radical production are major components in the pathogenesis of NSAID-induced small intestinal injury. We investigated the effect of GSPEs on indomethacin-induced intestinal mucosal injury in the rat. Rats were allocated into four groups: the control group, the indomethacin control group, the low-dose GSPEs group, and the high-dose GSPEs group. GSPEs were administered for 4 days. Then indomethacin and GSPEs were coadministered for the following 2 days by oral route. The dose of indomethacin was 200 mg/Kg. The doses of GSPEs were 100 mg/Kg for low-dose group and 300 mg/Kg for high-dose group. Luminal bleeding was solely observed in one of 5 rats from indomethacin control group. The number of ulcer count was reduced to 0.1 ± 0.3 per rat in GSPEs treated group compared to 1.4 ± 0.5 per rat in indomethacin control group. Submucosal inflammatory cell infiltration was also reduced to 50% in GSPEs treated group. The tissue level of prostaglandin E2 was not affected by GSPEs treatment. GSPEs attenuated the indomethacin-induced small intestinal injury irrespective of the tissue PGE2 depletion and glutathione consumption.

No MeSH data available.


Related in: MedlinePlus

Areas and numbers of ulcers and erosions. (a) The mean number ± SD of ulcers was 1.4 ± 0.5/rat in the indomethacin control group, zero in the GSPE low-dose group, and 0.2 ± 0.4/rat in the GSPE high-dose group (P value <0.001). The number of erosions was not significantly different among the treatment groups. (b) The mean area ± SD of mucosal defects was 11.1 ± 10.5 mm2/rat in the indomethacin control group, 3.9 ± 8.7 mm2/rat in the GSPE low-dose group, and 0.3 ± 0.4 mm2/rat in the GSPE high-dose group.
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fig5: Areas and numbers of ulcers and erosions. (a) The mean number ± SD of ulcers was 1.4 ± 0.5/rat in the indomethacin control group, zero in the GSPE low-dose group, and 0.2 ± 0.4/rat in the GSPE high-dose group (P value <0.001). The number of erosions was not significantly different among the treatment groups. (b) The mean area ± SD of mucosal defects was 11.1 ± 10.5 mm2/rat in the indomethacin control group, 3.9 ± 8.7 mm2/rat in the GSPE low-dose group, and 0.3 ± 0.4 mm2/rat in the GSPE high-dose group.

Mentions: More severe mucosal injury by indomethacin administration presented as erosions and ulcers. The number of ulcers per rat varied according to whether GSPEs administration was given (P value <0.001). The number of ulcers per rat was 1.4 ± 0.5 for the indomethacin control group and 0.2 ± 0.4 for the GSPEs high-dose group. None of the rats from the control group or the GSPEs low-dose group had ulcers. Compared to the indomethacin control group and rats treated with GSPEs, the number of ulcers per rat was significantly higher in the indomethacin group (1.4 ± 0.5 versus 0.1 ± 0.3, P value = 0.004). The number of erosions per rat was 1.6 ± 2.2 for the indomethacin control group, 0.4 ± 0.5 for the GSPEs low-dose group, and 0.8 ± 0.8 for the GSPEs high-dose group (P value = 0.35) (Figure 5(a)). There was no difference between the GSPEs high- and low-dose groups.


Proanthocyanidin from grape seed extracts protects indomethacin-induced small intestinal mucosal injury.

Cheung DY, Kim JI, Park SH, Kim JK - Gastroenterol Res Pract (2014)

Areas and numbers of ulcers and erosions. (a) The mean number ± SD of ulcers was 1.4 ± 0.5/rat in the indomethacin control group, zero in the GSPE low-dose group, and 0.2 ± 0.4/rat in the GSPE high-dose group (P value <0.001). The number of erosions was not significantly different among the treatment groups. (b) The mean area ± SD of mucosal defects was 11.1 ± 10.5 mm2/rat in the indomethacin control group, 3.9 ± 8.7 mm2/rat in the GSPE low-dose group, and 0.3 ± 0.4 mm2/rat in the GSPE high-dose group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig5: Areas and numbers of ulcers and erosions. (a) The mean number ± SD of ulcers was 1.4 ± 0.5/rat in the indomethacin control group, zero in the GSPE low-dose group, and 0.2 ± 0.4/rat in the GSPE high-dose group (P value <0.001). The number of erosions was not significantly different among the treatment groups. (b) The mean area ± SD of mucosal defects was 11.1 ± 10.5 mm2/rat in the indomethacin control group, 3.9 ± 8.7 mm2/rat in the GSPE low-dose group, and 0.3 ± 0.4 mm2/rat in the GSPE high-dose group.
Mentions: More severe mucosal injury by indomethacin administration presented as erosions and ulcers. The number of ulcers per rat varied according to whether GSPEs administration was given (P value <0.001). The number of ulcers per rat was 1.4 ± 0.5 for the indomethacin control group and 0.2 ± 0.4 for the GSPEs high-dose group. None of the rats from the control group or the GSPEs low-dose group had ulcers. Compared to the indomethacin control group and rats treated with GSPEs, the number of ulcers per rat was significantly higher in the indomethacin group (1.4 ± 0.5 versus 0.1 ± 0.3, P value = 0.004). The number of erosions per rat was 1.6 ± 2.2 for the indomethacin control group, 0.4 ± 0.5 for the GSPEs low-dose group, and 0.8 ± 0.8 for the GSPEs high-dose group (P value = 0.35) (Figure 5(a)). There was no difference between the GSPEs high- and low-dose groups.

Bottom Line: The number of ulcer count was reduced to 0.1 ± 0.3 per rat in GSPEs treated group compared to 1.4 ± 0.5 per rat in indomethacin control group.Submucosal inflammatory cell infiltration was also reduced to 50% in GSPEs treated group.The tissue level of prostaglandin E2 was not affected by GSPEs treatment.

View Article: PubMed Central - PubMed

Affiliation: The Department of Internal Medicine, The Catholic University of Korea College of Medicine, St. Mary's Hospital, Yeongdeungpo-gu 63-ro 10, Seoul 150-713, Republic of Korea.

ABSTRACT
Proanthocyanidin (grape seed proanthocyanidin extracts, GSPEs) is an antioxidant and scavenges free radicals. Excessive oxidative stress and free radical production are major components in the pathogenesis of NSAID-induced small intestinal injury. We investigated the effect of GSPEs on indomethacin-induced intestinal mucosal injury in the rat. Rats were allocated into four groups: the control group, the indomethacin control group, the low-dose GSPEs group, and the high-dose GSPEs group. GSPEs were administered for 4 days. Then indomethacin and GSPEs were coadministered for the following 2 days by oral route. The dose of indomethacin was 200 mg/Kg. The doses of GSPEs were 100 mg/Kg for low-dose group and 300 mg/Kg for high-dose group. Luminal bleeding was solely observed in one of 5 rats from indomethacin control group. The number of ulcer count was reduced to 0.1 ± 0.3 per rat in GSPEs treated group compared to 1.4 ± 0.5 per rat in indomethacin control group. Submucosal inflammatory cell infiltration was also reduced to 50% in GSPEs treated group. The tissue level of prostaglandin E2 was not affected by GSPEs treatment. GSPEs attenuated the indomethacin-induced small intestinal injury irrespective of the tissue PGE2 depletion and glutathione consumption.

No MeSH data available.


Related in: MedlinePlus