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Myrcia bella Leaf Extract Presents Hypoglycemic Activity via PI3k/Akt Insulin Signaling Pathway.

Vareda PM, Saldanha LL, Camaforte NA, Violato NM, Dokkedal AL, Bosqueiro JR - Evid Based Complement Alternat Med (2014)

Bottom Line: At the end of the treatment, blood insulin, triglycerides, total cholesterol, and protein were measured.Glycogen content and expression of proteins of the insulin signaling pathway were measured in liver.The results indicate that the extract of the leaves of Myrcia bella has hypoglycemic properties and possibly acts to regulate glucose uptake by the liver.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biosciences, São Paulo State University (UNESP), 18618-970 Botucatu, SP, Brazil.

ABSTRACT
Species of Myrcia are used by indigenous people and in traditional communities in Brazil for the treatment of Diabetes mellitus. We investigated the hypoglycemic effect of the extract of leaves of Myrcia bella in diabetic mice. The chemical fingerprinting of the 70% EtOH extract characterized as main constituents flavonoid aglycones, flavonoid-O-glycosides, and acylated flavonoid-O-glycosides derivatives of quercetin and myricetin. Mice were treated with saline or extract of M. bella (300 or 600 mg/Kg b.w.) for 14 days. Body weight and water and food intake were measured every day. Fasting blood glucose was measured weekly. At the end of the treatment, blood insulin, triglycerides, total cholesterol, and protein were measured. Glycogen content and expression of proteins of the insulin signaling pathway were measured in liver. The treatment with 600 mg/Kg reduced the fasting blood glucose in diabetic mice of the 7th day as water and food intake and increased hepatic glycogen. Total cholesterol and triglycerides were reduced in diabetic treated mice. The treatment increased the expression of IRS-1, PI3-K, and AKT in the livers of diabetic treated mice. The results indicate that the extract of the leaves of Myrcia bella has hypoglycemic properties and possibly acts to regulate glucose uptake by the liver.

No MeSH data available.


Related in: MedlinePlus

Intraperitoneal glucose tolerance test. Glycemia after intraperitoneal glucose administration (2 g/Kg) (a) and area under the curve (AUC) (b) in control and diabetic mice treated with saline (CTL SAL and STZ SAL) or with 600 mg/Kg of the crude extract of M. bella (CTL EXT and STZ EXT) during 14 days (CTL EXT and STZ EXT). Data are Means ± SEM. ∗ versus STZ SAL; @ versus CTL SAL, & versus CTL EXT, n = 8, P < 0.05. ANOVA followed by Tukey's posttest.
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fig4: Intraperitoneal glucose tolerance test. Glycemia after intraperitoneal glucose administration (2 g/Kg) (a) and area under the curve (AUC) (b) in control and diabetic mice treated with saline (CTL SAL and STZ SAL) or with 600 mg/Kg of the crude extract of M. bella (CTL EXT and STZ EXT) during 14 days (CTL EXT and STZ EXT). Data are Means ± SEM. ∗ versus STZ SAL; @ versus CTL SAL, & versus CTL EXT, n = 8, P < 0.05. ANOVA followed by Tukey's posttest.

Mentions: The ipGTT was performed according to the effective hypoglycemic dose of the extract (600 mg/Kg). Diabetic animals treated with the extract (STZ EXT) showed a significant decrease in glycemia at 30, 60, and 90 minutes (P < 0.05) after glucose administration (2 g/Kg) compared with diabetic animals treated with saline (STZ SAL) (Figure 4(a)). The area under the curve (AUC) (Figure 4(b)) was increased in both diabetic groups when compared with the control groups (CTL SAL and CTL EXT) (P < 0.001). Nevertheless, there was a decrease in AUC of the STZ EXT group when compared with the STZ SAL group (P < 0.05). There was no difference in blood glucose and AUC between the control groups.


Myrcia bella Leaf Extract Presents Hypoglycemic Activity via PI3k/Akt Insulin Signaling Pathway.

Vareda PM, Saldanha LL, Camaforte NA, Violato NM, Dokkedal AL, Bosqueiro JR - Evid Based Complement Alternat Med (2014)

Intraperitoneal glucose tolerance test. Glycemia after intraperitoneal glucose administration (2 g/Kg) (a) and area under the curve (AUC) (b) in control and diabetic mice treated with saline (CTL SAL and STZ SAL) or with 600 mg/Kg of the crude extract of M. bella (CTL EXT and STZ EXT) during 14 days (CTL EXT and STZ EXT). Data are Means ± SEM. ∗ versus STZ SAL; @ versus CTL SAL, & versus CTL EXT, n = 8, P < 0.05. ANOVA followed by Tukey's posttest.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4020406&req=5

fig4: Intraperitoneal glucose tolerance test. Glycemia after intraperitoneal glucose administration (2 g/Kg) (a) and area under the curve (AUC) (b) in control and diabetic mice treated with saline (CTL SAL and STZ SAL) or with 600 mg/Kg of the crude extract of M. bella (CTL EXT and STZ EXT) during 14 days (CTL EXT and STZ EXT). Data are Means ± SEM. ∗ versus STZ SAL; @ versus CTL SAL, & versus CTL EXT, n = 8, P < 0.05. ANOVA followed by Tukey's posttest.
Mentions: The ipGTT was performed according to the effective hypoglycemic dose of the extract (600 mg/Kg). Diabetic animals treated with the extract (STZ EXT) showed a significant decrease in glycemia at 30, 60, and 90 minutes (P < 0.05) after glucose administration (2 g/Kg) compared with diabetic animals treated with saline (STZ SAL) (Figure 4(a)). The area under the curve (AUC) (Figure 4(b)) was increased in both diabetic groups when compared with the control groups (CTL SAL and CTL EXT) (P < 0.001). Nevertheless, there was a decrease in AUC of the STZ EXT group when compared with the STZ SAL group (P < 0.05). There was no difference in blood glucose and AUC between the control groups.

Bottom Line: At the end of the treatment, blood insulin, triglycerides, total cholesterol, and protein were measured.Glycogen content and expression of proteins of the insulin signaling pathway were measured in liver.The results indicate that the extract of the leaves of Myrcia bella has hypoglycemic properties and possibly acts to regulate glucose uptake by the liver.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biosciences, São Paulo State University (UNESP), 18618-970 Botucatu, SP, Brazil.

ABSTRACT
Species of Myrcia are used by indigenous people and in traditional communities in Brazil for the treatment of Diabetes mellitus. We investigated the hypoglycemic effect of the extract of leaves of Myrcia bella in diabetic mice. The chemical fingerprinting of the 70% EtOH extract characterized as main constituents flavonoid aglycones, flavonoid-O-glycosides, and acylated flavonoid-O-glycosides derivatives of quercetin and myricetin. Mice were treated with saline or extract of M. bella (300 or 600 mg/Kg b.w.) for 14 days. Body weight and water and food intake were measured every day. Fasting blood glucose was measured weekly. At the end of the treatment, blood insulin, triglycerides, total cholesterol, and protein were measured. Glycogen content and expression of proteins of the insulin signaling pathway were measured in liver. The treatment with 600 mg/Kg reduced the fasting blood glucose in diabetic mice of the 7th day as water and food intake and increased hepatic glycogen. Total cholesterol and triglycerides were reduced in diabetic treated mice. The treatment increased the expression of IRS-1, PI3-K, and AKT in the livers of diabetic treated mice. The results indicate that the extract of the leaves of Myrcia bella has hypoglycemic properties and possibly acts to regulate glucose uptake by the liver.

No MeSH data available.


Related in: MedlinePlus