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Electroacupuncture Ameliorates Acute Lung Injury through Promoting Gastrointestinal Motility in Rats with Acute Pancreatitis.

Guo H, Zhu SF, Zhang RR, Zhao XL, Wan MH, Tang WF - Evid Based Complement Alternat Med (2014)

Bottom Line: EA treatment improved IPR and increased CCK-8 level compared with model group (P < 0.05).EA treatment reduced serum vasoactive intestinal peptide (VIP) and myeloperoxidase (MPO) level in the lung and the pathologic scores of pancreas, lung and intestine were decreased (P < 0.05).Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan Province 610041, China.

ABSTRACT
Objective. Gastrointestinal disfunction and acute lung injury (ALI) were common in acute pancreatitis (AP). The effect of electro-acupuncture (EA) on gastrointestinal motility and ALI in rats with AP was investigated to verify the theory of "lung and large intestine are interior exteriorly related" in traditional Chinese medicine. Methods. Male Sprague-Dawley rats were randomly divided into the normal group, model group, and EA group. AP model was established by three injections of 20% L-arginine at 1 h intervals. EA were applied to bilateral ST-25 and ST-36 for 30 minutes twice a day after modeling for 3 days. Arterial blood, pancreas, lung, and intestinal tissues were collected for detecting the inflammatory factors and histopathology. Intestinal propulsion rate (IPR) was also measured at 72 h. Results. EA treatment improved IPR and increased CCK-8 level compared with model group (P < 0.05). It lowered the serum levels of TNF- α and IL-6 and increased the level of IL-4 with no effect on IL-10. EA treatment reduced serum vasoactive intestinal peptide (VIP) and myeloperoxidase (MPO) level in the lung and the pathologic scores of pancreas, lung and intestine were decreased (P < 0.05). Conclusion. EA treatment could promote gastrointestinal motility through inhibiting VIP, and promoting CCK expression and regulate pro- and anti-inflammatory mediators to ameliorate ALI in AP.

No MeSH data available.


Related in: MedlinePlus

Comparison of serum TNF-α, IL-6, IL-4, and IL-10 among groups (mean ± SD) pg/mL.
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Related In: Results  -  Collection


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fig3: Comparison of serum TNF-α, IL-6, IL-4, and IL-10 among groups (mean ± SD) pg/mL.

Mentions: As shown in Figure 3, serum levels of proinflammatory TNF-α and IL-6 were increased (Figures 3(a) and 3(b)) and anti-inflammatory IL-4 and IL-10 were decreased after induction of AP in rates (Figures 3(c) and 3(d)). Compared to the model rats, EA could decrease the serum levels of TNF-α and IL-6 significantly after 2 days of treatment (Figures 3(a) and 3(b)). EA treatment could also increase IL-4 levels in rates with SAP significantly (Figure 3(c)), with no effect on IL-10 (Figure 3(d)). All these data displayed that EA treatment could regulate the inflammatory response in rats with AP via inhibiting the proinflammatory mediators and promoting the anti-inflammatory mediator.


Electroacupuncture Ameliorates Acute Lung Injury through Promoting Gastrointestinal Motility in Rats with Acute Pancreatitis.

Guo H, Zhu SF, Zhang RR, Zhao XL, Wan MH, Tang WF - Evid Based Complement Alternat Med (2014)

Comparison of serum TNF-α, IL-6, IL-4, and IL-10 among groups (mean ± SD) pg/mL.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4020394&req=5

fig3: Comparison of serum TNF-α, IL-6, IL-4, and IL-10 among groups (mean ± SD) pg/mL.
Mentions: As shown in Figure 3, serum levels of proinflammatory TNF-α and IL-6 were increased (Figures 3(a) and 3(b)) and anti-inflammatory IL-4 and IL-10 were decreased after induction of AP in rates (Figures 3(c) and 3(d)). Compared to the model rats, EA could decrease the serum levels of TNF-α and IL-6 significantly after 2 days of treatment (Figures 3(a) and 3(b)). EA treatment could also increase IL-4 levels in rates with SAP significantly (Figure 3(c)), with no effect on IL-10 (Figure 3(d)). All these data displayed that EA treatment could regulate the inflammatory response in rats with AP via inhibiting the proinflammatory mediators and promoting the anti-inflammatory mediator.

Bottom Line: EA treatment improved IPR and increased CCK-8 level compared with model group (P < 0.05).EA treatment reduced serum vasoactive intestinal peptide (VIP) and myeloperoxidase (MPO) level in the lung and the pathologic scores of pancreas, lung and intestine were decreased (P < 0.05).Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan Province 610041, China.

ABSTRACT
Objective. Gastrointestinal disfunction and acute lung injury (ALI) were common in acute pancreatitis (AP). The effect of electro-acupuncture (EA) on gastrointestinal motility and ALI in rats with AP was investigated to verify the theory of "lung and large intestine are interior exteriorly related" in traditional Chinese medicine. Methods. Male Sprague-Dawley rats were randomly divided into the normal group, model group, and EA group. AP model was established by three injections of 20% L-arginine at 1 h intervals. EA were applied to bilateral ST-25 and ST-36 for 30 minutes twice a day after modeling for 3 days. Arterial blood, pancreas, lung, and intestinal tissues were collected for detecting the inflammatory factors and histopathology. Intestinal propulsion rate (IPR) was also measured at 72 h. Results. EA treatment improved IPR and increased CCK-8 level compared with model group (P < 0.05). It lowered the serum levels of TNF- α and IL-6 and increased the level of IL-4 with no effect on IL-10. EA treatment reduced serum vasoactive intestinal peptide (VIP) and myeloperoxidase (MPO) level in the lung and the pathologic scores of pancreas, lung and intestine were decreased (P < 0.05). Conclusion. EA treatment could promote gastrointestinal motility through inhibiting VIP, and promoting CCK expression and regulate pro- and anti-inflammatory mediators to ameliorate ALI in AP.

No MeSH data available.


Related in: MedlinePlus