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Humoral autoimmune responses to insulin-like growth factor II mRNA-binding proteins IMP1 and p62/IMP2 in ovarian cancer.

Liu X, Ye H, Li L, Li W, Zhang Y, Zhang JY - J Immunol Res (2014)

Bottom Line: There is an urgent need of better approaches for the identification of appropriate biomarkers in the early detection of ovarian cancer.The aim of this study was to elucidate the significance of autoantibodies against insulin-like growth factor II mRNA-binding proteins (IMPs) in patients with ovarian cancer.The results have demonstrated that both IMP1 and p62/IMP2 can induce relatively higher frequency of autoantibody responses in patients with ovarian cancer (26.5% and 29.4%) compared to normal individuals (P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Center for Tumor Biotherapy, The First Affiliated Hospital & College of Public Health, Zhengzhou University, Zhengzhou, Henan 450052, China ; Department of Biological Sciences & Border Biomedical Research Center, The University of Texas at El Paso, El Paso, TX 79968, USA.

ABSTRACT
Ovarian cancer is one of the leading causes of cancer-related deaths among women. There is an urgent need of better approaches for the identification of appropriate biomarkers in the early detection of ovarian cancer. The aim of this study was to elucidate the significance of autoantibodies against insulin-like growth factor II mRNA-binding proteins (IMPs) in patients with ovarian cancer. In this study, autoantibody responses to two members (IMP1 and p62/IMP2) of IMPs were evaluated by enzyme-linked immunosorbent assay (ELISA), western blotting, and indirect immunofluorescence assay in sera from patients with ovarian cancer and normal human individuals. The results have demonstrated that both IMP1 and p62/IMP2 can induce relatively higher frequency of autoantibody responses in patients with ovarian cancer (26.5% and 29.4%) compared to normal individuals (P<0.01). Our preliminary data suggest that IMP1 and p62/IMP2 can stimulate autoimmune responses in ovarian cancer, and anti-IMP1 and anti-p62/IMP2 autoantibodies could be used as potential biomarkers in immunodiagnosis of ovarian cancer.

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Related in: MedlinePlus

Western blotting analysis showing representative ovarian cancer sera recognizing IMP1 and p62/IMP2 recombinant proteins. The monoclonal anti-IMP1 and anti-p62/IMP2 antibodies were used as positive controls; lanes 1–4, four representative ovarian cancer sera that were positive in ELISA test and also have strong reactivity with IMP1 and p62/IMP2 recombinant proteins in Western blotting analysis; lanes 5 and 6, normal human sera that were used as negative control.
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fig2: Western blotting analysis showing representative ovarian cancer sera recognizing IMP1 and p62/IMP2 recombinant proteins. The monoclonal anti-IMP1 and anti-p62/IMP2 antibodies were used as positive controls; lanes 1–4, four representative ovarian cancer sera that were positive in ELISA test and also have strong reactivity with IMP1 and p62/IMP2 recombinant proteins in Western blotting analysis; lanes 5 and 6, normal human sera that were used as negative control.

Mentions: Serum levels of anti-IMP1 and anti-p62/IMP2 autoantibodies were determined by ELISA as described in the section of Materials and Methods. In total, 34 sera from patients with ovarian cancer and 89 sera from normal human individuals were used in this study. As shown in Table 1, the prevalence of autoantibody against IMP1 was 26.5% (9/34) in ovarian cancer, which was significantly higher than that in NHS (1.1%, 1/89) (P < 0.01). Titer of anti-IMP1 antibody in human sera was shown in Figure 1. The average titer of autoantibody against IMP1 in ovarian cancer sera was higher than that in NHS (P < 0.01). As demonstrated in Table 1, the frequency of autoantibody to p62/IMP2 was 29.4% (10/34), which was significantly higher than that in NHS (1.1%, 1/89). Titer of anti-p62/IMP2 antibody in human sera was shown in Figure 1. The average titer of autoantibody against anti-p62/IMP2 in ovarian cancer sera was higher than that in NHS (P < 0.01). The ELISA results were also confirmed by western blot analysis. Figure 2 showed that representative ovarian cancer sera with positive reaction to IMP1 and p62/IMP2 in ELISA also have strong reactivity in western blotting compared to normal sera.


Humoral autoimmune responses to insulin-like growth factor II mRNA-binding proteins IMP1 and p62/IMP2 in ovarian cancer.

Liu X, Ye H, Li L, Li W, Zhang Y, Zhang JY - J Immunol Res (2014)

Western blotting analysis showing representative ovarian cancer sera recognizing IMP1 and p62/IMP2 recombinant proteins. The monoclonal anti-IMP1 and anti-p62/IMP2 antibodies were used as positive controls; lanes 1–4, four representative ovarian cancer sera that were positive in ELISA test and also have strong reactivity with IMP1 and p62/IMP2 recombinant proteins in Western blotting analysis; lanes 5 and 6, normal human sera that were used as negative control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4020369&req=5

fig2: Western blotting analysis showing representative ovarian cancer sera recognizing IMP1 and p62/IMP2 recombinant proteins. The monoclonal anti-IMP1 and anti-p62/IMP2 antibodies were used as positive controls; lanes 1–4, four representative ovarian cancer sera that were positive in ELISA test and also have strong reactivity with IMP1 and p62/IMP2 recombinant proteins in Western blotting analysis; lanes 5 and 6, normal human sera that were used as negative control.
Mentions: Serum levels of anti-IMP1 and anti-p62/IMP2 autoantibodies were determined by ELISA as described in the section of Materials and Methods. In total, 34 sera from patients with ovarian cancer and 89 sera from normal human individuals were used in this study. As shown in Table 1, the prevalence of autoantibody against IMP1 was 26.5% (9/34) in ovarian cancer, which was significantly higher than that in NHS (1.1%, 1/89) (P < 0.01). Titer of anti-IMP1 antibody in human sera was shown in Figure 1. The average titer of autoantibody against IMP1 in ovarian cancer sera was higher than that in NHS (P < 0.01). As demonstrated in Table 1, the frequency of autoantibody to p62/IMP2 was 29.4% (10/34), which was significantly higher than that in NHS (1.1%, 1/89). Titer of anti-p62/IMP2 antibody in human sera was shown in Figure 1. The average titer of autoantibody against anti-p62/IMP2 in ovarian cancer sera was higher than that in NHS (P < 0.01). The ELISA results were also confirmed by western blot analysis. Figure 2 showed that representative ovarian cancer sera with positive reaction to IMP1 and p62/IMP2 in ELISA also have strong reactivity in western blotting compared to normal sera.

Bottom Line: There is an urgent need of better approaches for the identification of appropriate biomarkers in the early detection of ovarian cancer.The aim of this study was to elucidate the significance of autoantibodies against insulin-like growth factor II mRNA-binding proteins (IMPs) in patients with ovarian cancer.The results have demonstrated that both IMP1 and p62/IMP2 can induce relatively higher frequency of autoantibody responses in patients with ovarian cancer (26.5% and 29.4%) compared to normal individuals (P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Center for Tumor Biotherapy, The First Affiliated Hospital & College of Public Health, Zhengzhou University, Zhengzhou, Henan 450052, China ; Department of Biological Sciences & Border Biomedical Research Center, The University of Texas at El Paso, El Paso, TX 79968, USA.

ABSTRACT
Ovarian cancer is one of the leading causes of cancer-related deaths among women. There is an urgent need of better approaches for the identification of appropriate biomarkers in the early detection of ovarian cancer. The aim of this study was to elucidate the significance of autoantibodies against insulin-like growth factor II mRNA-binding proteins (IMPs) in patients with ovarian cancer. In this study, autoantibody responses to two members (IMP1 and p62/IMP2) of IMPs were evaluated by enzyme-linked immunosorbent assay (ELISA), western blotting, and indirect immunofluorescence assay in sera from patients with ovarian cancer and normal human individuals. The results have demonstrated that both IMP1 and p62/IMP2 can induce relatively higher frequency of autoantibody responses in patients with ovarian cancer (26.5% and 29.4%) compared to normal individuals (P<0.01). Our preliminary data suggest that IMP1 and p62/IMP2 can stimulate autoimmune responses in ovarian cancer, and anti-IMP1 and anti-p62/IMP2 autoantibodies could be used as potential biomarkers in immunodiagnosis of ovarian cancer.

Show MeSH
Related in: MedlinePlus