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Identification of osteosarcoma-related specific proteins in serum samples using surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry.

Gu J, Li J, Huang M, Zhang Z, Li D, Song G, Ding X, Li W - J Immunol Res (2014)

Bottom Line: Two proteins showed a significantly different expression in OS serum samples from control groups.These two proteins were further identified by searching the EPO-KB database, and one of the proteins identified as Serine rich region profile is involved in various cellular signaling cascades and tumor genesis.The presence of these two proteins in OS patients but absence from premalignant and normal human controls implied that they can be potential biomarkers for early diagnosis of OS.

View Article: PubMed Central - PubMed

Affiliation: Luoyang Orthopedic-Traumatological Hospital, Luoyang Institute of Orthopedic and Traumatology, Henan Province, Luoyang 471002, China.

ABSTRACT
Osteosarcoma (OS) is the most common malignant bone tumor. To identify OS-related specific proteins for early diagnosis of OS, a novel approach, surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry (SELDI-TOF-MS) to serum samples from 25 OS patients, 16 osteochondroma, and 26 age-matched normal human volunteers as controls, was performed. Two proteins showed a significantly different expression in OS serum samples from control groups. Proteomic profiles and external leave-one-out cross-validation analysis showed that the correct rate of allocation, the sensitivity, and the specificity of diagnosis were 100%. These two proteins were further identified by searching the EPO-KB database, and one of the proteins identified as Serine rich region profile is involved in various cellular signaling cascades and tumor genesis. The presence of these two proteins in OS patients but absence from premalignant and normal human controls implied that they can be potential biomarkers for early diagnosis of OS.

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Related in: MedlinePlus

The protein intensity of osteochondroma (OC), osteosarcoma (OS), and normal human serum (NHS). The protein intensity of the serum samples from NHS (green panel), OS (blue panel), and OC (red panel).
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fig2: The protein intensity of osteochondroma (OC), osteosarcoma (OS), and normal human serum (NHS). The protein intensity of the serum samples from NHS (green panel), OS (blue panel), and OC (red panel).

Mentions: Sixty-seven serum samples were assayed by SELDI-TOF-MS. From a total of 67 serum samples, 25 serum samples are from OS patients compared with the control group of 42 serum samples (16 from osteochondroma and 26 from normal human serum). Interestingly, two protein peaks differed significantly in the OS patient group. One protein peak at 3954 Da was overexpressed and another one at 6438 Da (Table 1). The mass spectrum shows the comparison of three serum samples from patients with OS and OC as well as a serum from a normal individual (Figures 1 and 2).


Identification of osteosarcoma-related specific proteins in serum samples using surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry.

Gu J, Li J, Huang M, Zhang Z, Li D, Song G, Ding X, Li W - J Immunol Res (2014)

The protein intensity of osteochondroma (OC), osteosarcoma (OS), and normal human serum (NHS). The protein intensity of the serum samples from NHS (green panel), OS (blue panel), and OC (red panel).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4020367&req=5

fig2: The protein intensity of osteochondroma (OC), osteosarcoma (OS), and normal human serum (NHS). The protein intensity of the serum samples from NHS (green panel), OS (blue panel), and OC (red panel).
Mentions: Sixty-seven serum samples were assayed by SELDI-TOF-MS. From a total of 67 serum samples, 25 serum samples are from OS patients compared with the control group of 42 serum samples (16 from osteochondroma and 26 from normal human serum). Interestingly, two protein peaks differed significantly in the OS patient group. One protein peak at 3954 Da was overexpressed and another one at 6438 Da (Table 1). The mass spectrum shows the comparison of three serum samples from patients with OS and OC as well as a serum from a normal individual (Figures 1 and 2).

Bottom Line: Two proteins showed a significantly different expression in OS serum samples from control groups.These two proteins were further identified by searching the EPO-KB database, and one of the proteins identified as Serine rich region profile is involved in various cellular signaling cascades and tumor genesis.The presence of these two proteins in OS patients but absence from premalignant and normal human controls implied that they can be potential biomarkers for early diagnosis of OS.

View Article: PubMed Central - PubMed

Affiliation: Luoyang Orthopedic-Traumatological Hospital, Luoyang Institute of Orthopedic and Traumatology, Henan Province, Luoyang 471002, China.

ABSTRACT
Osteosarcoma (OS) is the most common malignant bone tumor. To identify OS-related specific proteins for early diagnosis of OS, a novel approach, surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry (SELDI-TOF-MS) to serum samples from 25 OS patients, 16 osteochondroma, and 26 age-matched normal human volunteers as controls, was performed. Two proteins showed a significantly different expression in OS serum samples from control groups. Proteomic profiles and external leave-one-out cross-validation analysis showed that the correct rate of allocation, the sensitivity, and the specificity of diagnosis were 100%. These two proteins were further identified by searching the EPO-KB database, and one of the proteins identified as Serine rich region profile is involved in various cellular signaling cascades and tumor genesis. The presence of these two proteins in OS patients but absence from premalignant and normal human controls implied that they can be potential biomarkers for early diagnosis of OS.

Show MeSH
Related in: MedlinePlus