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Enhanced Photosynthesis and Carbon Metabolism Favor Arsenic Tolerance in Artemisia annua, a Medicinal Plant as Revealed by Homology-Based Proteomics.

Rai R, Pandey S, Shrivastava AK, Pandey Rai S - Int J Proteomics (2014)

Bottom Line: However, a decrease in the above variables was recorded under 150  μ M treatments.While an increased accumulation of ATP synthase, ferredoxin-NADP(H) oxidoreductase, and FeS-rieske proteins supported the operation of cyclic electron transport, mdr ABC transporter protein and pcs gene might be involved in As detoxification.This study not only affirmed the role of energy metabolism proteins but also identified potential candidates responsible for As tolerance in plants.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Morphogenesis, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.

ABSTRACT
This paper provides the first proteomic evidence of arsenic (As) tolerance and interactive regulatory network between primary and secondary metabolism in the medicinal plant, Artemisia annua. While chlorophyll fluorescence and photosynthetic rate depicted mild inhibition, there was a significant enhancement in PSI activity, whole chain, ATP, and NADPH contents in 100  μ M As treatments compared to the control plants. However, a decrease in the above variables was recorded under 150  μ M treatments. Proteomic decoding of the survival strategy of A. annua under As stress using 2-DE followed by MALDI-MS/MS revealed a total of 46 differentially expressed protein spots. In contrast to other plants where As inhibits photosynthesis, A. annua showed appreciable photosynthetic CO2 assimilation and allocation of carbon resources at 100  μ M As concentration. While an increased accumulation of ATP synthase, ferredoxin-NADP(H) oxidoreductase, and FeS-rieske proteins supported the operation of cyclic electron transport, mdr ABC transporter protein and pcs gene might be involved in As detoxification. The most interesting observation was an increased accumulation of LEAFY like novel protein conceivably responsible for an early onset of flowering in A. annua under As stress. This study not only affirmed the role of energy metabolism proteins but also identified potential candidates responsible for As tolerance in plants.

No MeSH data available.


Related in: MedlinePlus

Western blot of PCS protein after treatment with 100 and 150 μM As concentrations. Protein separated by SDS-PAGE, electroblotted onto a PVDF membrane, and cross-reacted with primary antibody (anti-PCS antibody).
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fig6: Western blot of PCS protein after treatment with 100 and 150 μM As concentrations. Protein separated by SDS-PAGE, electroblotted onto a PVDF membrane, and cross-reacted with primary antibody (anti-PCS antibody).

Mentions: Of particular interest are the multidrug resistance-associated proteins (MRPs), a subclass of ATP-binding cassette (ABC) transporters with a hypothetical role in As extrusion in plant. Our previous finding [11] impelled to check the role of pcs gene in As detoxification. The upregulated (1.5-fold) mdr-like ABC transporter and pcs gene (as confirmed by Western blot analysis, Figure 6) under 100 μM As suggest that detoxification was brought about by reduction of As(V) to As(III) and sequestration with phytochelatin. The increased abundance of (ABC) transporters has also been reported in Comamonas sp. under As stress [81]. Another interesting observation was the increased accumulation of S-adenosyl methionine synthase (SAMS), known to act as a signaling molecule and protects cells from arsenic stress by forming S-adenosyl-methionine [20]. Furthermore, in the light of the report of Noriega et al. [82] that SAM protects cells from deleterious effects of cadmium, its increased accumulation under 100 μM As treatment seems justified.


Enhanced Photosynthesis and Carbon Metabolism Favor Arsenic Tolerance in Artemisia annua, a Medicinal Plant as Revealed by Homology-Based Proteomics.

Rai R, Pandey S, Shrivastava AK, Pandey Rai S - Int J Proteomics (2014)

Western blot of PCS protein after treatment with 100 and 150 μM As concentrations. Protein separated by SDS-PAGE, electroblotted onto a PVDF membrane, and cross-reacted with primary antibody (anti-PCS antibody).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4020366&req=5

fig6: Western blot of PCS protein after treatment with 100 and 150 μM As concentrations. Protein separated by SDS-PAGE, electroblotted onto a PVDF membrane, and cross-reacted with primary antibody (anti-PCS antibody).
Mentions: Of particular interest are the multidrug resistance-associated proteins (MRPs), a subclass of ATP-binding cassette (ABC) transporters with a hypothetical role in As extrusion in plant. Our previous finding [11] impelled to check the role of pcs gene in As detoxification. The upregulated (1.5-fold) mdr-like ABC transporter and pcs gene (as confirmed by Western blot analysis, Figure 6) under 100 μM As suggest that detoxification was brought about by reduction of As(V) to As(III) and sequestration with phytochelatin. The increased abundance of (ABC) transporters has also been reported in Comamonas sp. under As stress [81]. Another interesting observation was the increased accumulation of S-adenosyl methionine synthase (SAMS), known to act as a signaling molecule and protects cells from arsenic stress by forming S-adenosyl-methionine [20]. Furthermore, in the light of the report of Noriega et al. [82] that SAM protects cells from deleterious effects of cadmium, its increased accumulation under 100 μM As treatment seems justified.

Bottom Line: However, a decrease in the above variables was recorded under 150  μ M treatments.While an increased accumulation of ATP synthase, ferredoxin-NADP(H) oxidoreductase, and FeS-rieske proteins supported the operation of cyclic electron transport, mdr ABC transporter protein and pcs gene might be involved in As detoxification.This study not only affirmed the role of energy metabolism proteins but also identified potential candidates responsible for As tolerance in plants.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Morphogenesis, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.

ABSTRACT
This paper provides the first proteomic evidence of arsenic (As) tolerance and interactive regulatory network between primary and secondary metabolism in the medicinal plant, Artemisia annua. While chlorophyll fluorescence and photosynthetic rate depicted mild inhibition, there was a significant enhancement in PSI activity, whole chain, ATP, and NADPH contents in 100  μ M As treatments compared to the control plants. However, a decrease in the above variables was recorded under 150  μ M treatments. Proteomic decoding of the survival strategy of A. annua under As stress using 2-DE followed by MALDI-MS/MS revealed a total of 46 differentially expressed protein spots. In contrast to other plants where As inhibits photosynthesis, A. annua showed appreciable photosynthetic CO2 assimilation and allocation of carbon resources at 100  μ M As concentration. While an increased accumulation of ATP synthase, ferredoxin-NADP(H) oxidoreductase, and FeS-rieske proteins supported the operation of cyclic electron transport, mdr ABC transporter protein and pcs gene might be involved in As detoxification. The most interesting observation was an increased accumulation of LEAFY like novel protein conceivably responsible for an early onset of flowering in A. annua under As stress. This study not only affirmed the role of energy metabolism proteins but also identified potential candidates responsible for As tolerance in plants.

No MeSH data available.


Related in: MedlinePlus