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Expression of tight junction protein claudin-1 in human crescentic glomerulonephritis.

Koda R, Yoshino A, Imanishi Y, Kawamoto S, Ueda Y, Yaoita E, Kazama JJ, Narita I, Takeda T - Int J Nephrol (2014)

Bottom Line: Colocalization of claudin-1 with ZO-1 was found at cell to cell contact sites of adjacent proliferating cells.Our findings suggest that claudin-1 contributes to crescent formation as a component of the tight junction protein complex that includes ZO-1.Co-localization of claudin-1 with ZO-1 implies the formation of functional tight junction complexes in crescentic lesions to prevent the interstitial damage caused by penetration of filtered molecules from Bowman's space.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Dokkyo Medical University Koshigaya Hospital, No. 1-50, 2-Chome, Minami-Koshigaya, Koshigaya-shi, Saitama 343-8555, Japan.

ABSTRACT
The origin of crescent forming cells in human glomerulonephritis (GN) remains unknown. Some animal studies demonstrated that parietal epithelial cells of Bowman's capsule (PECs) were the main component of proliferating cells and PEC-specific tight junction protein claudin-1 was expressed in crescentic lesions. We investigated the expression of claudin-1 in human GN. Immunohistochemistry for claudin-1 was performed on 17 kidney biopsy samples with crescent formation. Colocalization of claudin-1 with intracellular tight junction protein ZO-1 was also evaluated by immunofluorescence double staining. Claudin-1 is expressed mainly at the cell to cell contact site of proliferating cells in cellular crescentic lesions in patients with these forms of human GN. Small numbers of crescent forming cells showed extrajunctional localization of claudin-1. Colocalization of claudin-1 with ZO-1 was found at cell to cell contact sites of adjacent proliferating cells. In control samples, staining of claudin-1 was positive in PECs, but not in podocytes. Our findings suggest that claudin-1 contributes to crescent formation as a component of the tight junction protein complex that includes ZO-1. Co-localization of claudin-1 with ZO-1 implies the formation of functional tight junction complexes in crescentic lesions to prevent the interstitial damage caused by penetration of filtered molecules from Bowman's space.

No MeSH data available.


Related in: MedlinePlus

Transmission electron microscopy. Close contact between cell membranes of adjacent cells was observed, confirming the formation of tight junction in crescentic lesion in human glomerulonephritis ((b) arrowhead, sample from patient with IgA nephropathy). Figure (b) is magnified view of the framed area in (a).
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fig4: Transmission electron microscopy. Close contact between cell membranes of adjacent cells was observed, confirming the formation of tight junction in crescentic lesion in human glomerulonephritis ((b) arrowhead, sample from patient with IgA nephropathy). Figure (b) is magnified view of the framed area in (a).

Mentions: Under electron microscopy, tight junction structure (close contacts between cell membranes of adjacent cells) was confirmed in crescent forming cells (Figure 4, biopsy sample from patient with IgA nephropathy).


Expression of tight junction protein claudin-1 in human crescentic glomerulonephritis.

Koda R, Yoshino A, Imanishi Y, Kawamoto S, Ueda Y, Yaoita E, Kazama JJ, Narita I, Takeda T - Int J Nephrol (2014)

Transmission electron microscopy. Close contact between cell membranes of adjacent cells was observed, confirming the formation of tight junction in crescentic lesion in human glomerulonephritis ((b) arrowhead, sample from patient with IgA nephropathy). Figure (b) is magnified view of the framed area in (a).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4020360&req=5

fig4: Transmission electron microscopy. Close contact between cell membranes of adjacent cells was observed, confirming the formation of tight junction in crescentic lesion in human glomerulonephritis ((b) arrowhead, sample from patient with IgA nephropathy). Figure (b) is magnified view of the framed area in (a).
Mentions: Under electron microscopy, tight junction structure (close contacts between cell membranes of adjacent cells) was confirmed in crescent forming cells (Figure 4, biopsy sample from patient with IgA nephropathy).

Bottom Line: Colocalization of claudin-1 with ZO-1 was found at cell to cell contact sites of adjacent proliferating cells.Our findings suggest that claudin-1 contributes to crescent formation as a component of the tight junction protein complex that includes ZO-1.Co-localization of claudin-1 with ZO-1 implies the formation of functional tight junction complexes in crescentic lesions to prevent the interstitial damage caused by penetration of filtered molecules from Bowman's space.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Dokkyo Medical University Koshigaya Hospital, No. 1-50, 2-Chome, Minami-Koshigaya, Koshigaya-shi, Saitama 343-8555, Japan.

ABSTRACT
The origin of crescent forming cells in human glomerulonephritis (GN) remains unknown. Some animal studies demonstrated that parietal epithelial cells of Bowman's capsule (PECs) were the main component of proliferating cells and PEC-specific tight junction protein claudin-1 was expressed in crescentic lesions. We investigated the expression of claudin-1 in human GN. Immunohistochemistry for claudin-1 was performed on 17 kidney biopsy samples with crescent formation. Colocalization of claudin-1 with intracellular tight junction protein ZO-1 was also evaluated by immunofluorescence double staining. Claudin-1 is expressed mainly at the cell to cell contact site of proliferating cells in cellular crescentic lesions in patients with these forms of human GN. Small numbers of crescent forming cells showed extrajunctional localization of claudin-1. Colocalization of claudin-1 with ZO-1 was found at cell to cell contact sites of adjacent proliferating cells. In control samples, staining of claudin-1 was positive in PECs, but not in podocytes. Our findings suggest that claudin-1 contributes to crescent formation as a component of the tight junction protein complex that includes ZO-1. Co-localization of claudin-1 with ZO-1 implies the formation of functional tight junction complexes in crescentic lesions to prevent the interstitial damage caused by penetration of filtered molecules from Bowman's space.

No MeSH data available.


Related in: MedlinePlus