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Composition and cytotoxic activity of essential oils from Xylopia aethiopica (Dunal) A. Rich, Xylopia parviflora (A. Rich) Benth.) and Monodora myristica (Gaertn) growing in Chad and Cameroon.

Bakarnga-Via I, Hzounda JB, Fokou PV, Tchokouaha LR, Gary-Bobo M, Gallud A, Garcia M, Walbadet L, Secka Y, Dongmo PM, Boyom FF, Menut C - BMC Complement Altern Med (2014)

Bottom Line: Overall, monoterpenes were mostly found in the six essential oils.The plant origin did not significantly influence the chemical composition of oils.Essential oils from fruits of Monodora myristica, Xylopia aethiopica and Xylopia parviflora have shown acceptable antineoplastic potency, and might be investigated further in this regard.

View Article: PubMed Central - HTML - PubMed

Affiliation: Antimicrobial Agents Unit, Faculty of science, University of Yaoundé I, P,O Box 812, Yaoundé, Cameroon. fabrice.boyom@fulbrightmail.org.

ABSTRACT

Background: Cancer has become a global public health problem and the search for new control measures is urgent. Investigation of plant products such as essential oils from Monodora myristica, Xylopia aethiopica and Xylopia parviflora might lead to new anticancer therapy. In this study, we have investigated the antineoplastic activity of essential oils from fruits of these plants growing in Chad and Cameroon.

Methods: The essential oils obtained by hydrodistillation of fruits of Monodora myristica, Xylopia aethiopica and Xylopia parviflora collected in Chad and Cameroon were analyzed by GC-FID and GC-MS and investigated for their antiproliferative activity against the breast cancer cell line (MCF7).

Results: Overall, monoterpenes were mostly found in the six essential oils. Oils from X. aethiopica and X. parviflora from Chad and Cameroon mainly contain β-pinene at 24.6%, 28.2%, 35.7% and 32.9% respectively. Monodora myristica oils from both origins contain mainly α-phellandrene at 52.7% and 67.1% respectively. The plant origin did not significantly influence the chemical composition of oils. The six essential oils exerted cytotoxic activity against cancer (MCF-7) and normal cell lines (ARPE-19), with more pronounced effect on neoplastic cells in the majority of cases. The highest selectivity was obtained with the essential oils of X. parviflora from Chad and Cameroon (5.87 and 5.54) which were more cytotoxic against MCF-7 than against normal cell line (ARPE-19) with IC50 values of 0.155 μL/mL and 0.166 μL/mL respectively.

Conclusions: Essential oils from fruits of Monodora myristica, Xylopia aethiopica and Xylopia parviflora have shown acceptable antineoplastic potency, and might be investigated further in this regard.

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Dose-dependent cytotoxic effect of the essential oils of X. parviflora from Chad (A) and Cameroon (B), X. aethiopica from Chad (C) and Cameroon (D) and M. myristica from Chad (E) and Cameroon (F). Human breast cancer (MCF-7) and normal (ARPE-19) cell lines were incubated 72 h with increasing doses of essential oils from 0.1 to 2 μL/mL. After treatment, cytotoxicity was measured by MTT assay as described in Material and Methods. Values are means ± standard deviations of three independent experiments.
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Figure 1: Dose-dependent cytotoxic effect of the essential oils of X. parviflora from Chad (A) and Cameroon (B), X. aethiopica from Chad (C) and Cameroon (D) and M. myristica from Chad (E) and Cameroon (F). Human breast cancer (MCF-7) and normal (ARPE-19) cell lines were incubated 72 h with increasing doses of essential oils from 0.1 to 2 μL/mL. After treatment, cytotoxicity was measured by MTT assay as described in Material and Methods. Values are means ± standard deviations of three independent experiments.

Mentions: The cytotoxic effects of the essential oils on human normal or cancer cell lines are shown in Figure 1. The IC50 values and the selectivity indices were given in each case. The essential oils from fruits of X. aethiopica, X. parviflora and M. myristica produced a highly significant (p < 0.005) decrease in living cancer cells (MCF-7) after 72 h of incubation. Importantly, the effects on normal epithelial cells (ARPE-19) were mainly less pronounced.


Composition and cytotoxic activity of essential oils from Xylopia aethiopica (Dunal) A. Rich, Xylopia parviflora (A. Rich) Benth.) and Monodora myristica (Gaertn) growing in Chad and Cameroon.

Bakarnga-Via I, Hzounda JB, Fokou PV, Tchokouaha LR, Gary-Bobo M, Gallud A, Garcia M, Walbadet L, Secka Y, Dongmo PM, Boyom FF, Menut C - BMC Complement Altern Med (2014)

Dose-dependent cytotoxic effect of the essential oils of X. parviflora from Chad (A) and Cameroon (B), X. aethiopica from Chad (C) and Cameroon (D) and M. myristica from Chad (E) and Cameroon (F). Human breast cancer (MCF-7) and normal (ARPE-19) cell lines were incubated 72 h with increasing doses of essential oils from 0.1 to 2 μL/mL. After treatment, cytotoxicity was measured by MTT assay as described in Material and Methods. Values are means ± standard deviations of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4020318&req=5

Figure 1: Dose-dependent cytotoxic effect of the essential oils of X. parviflora from Chad (A) and Cameroon (B), X. aethiopica from Chad (C) and Cameroon (D) and M. myristica from Chad (E) and Cameroon (F). Human breast cancer (MCF-7) and normal (ARPE-19) cell lines were incubated 72 h with increasing doses of essential oils from 0.1 to 2 μL/mL. After treatment, cytotoxicity was measured by MTT assay as described in Material and Methods. Values are means ± standard deviations of three independent experiments.
Mentions: The cytotoxic effects of the essential oils on human normal or cancer cell lines are shown in Figure 1. The IC50 values and the selectivity indices were given in each case. The essential oils from fruits of X. aethiopica, X. parviflora and M. myristica produced a highly significant (p < 0.005) decrease in living cancer cells (MCF-7) after 72 h of incubation. Importantly, the effects on normal epithelial cells (ARPE-19) were mainly less pronounced.

Bottom Line: Overall, monoterpenes were mostly found in the six essential oils.The plant origin did not significantly influence the chemical composition of oils.Essential oils from fruits of Monodora myristica, Xylopia aethiopica and Xylopia parviflora have shown acceptable antineoplastic potency, and might be investigated further in this regard.

View Article: PubMed Central - HTML - PubMed

Affiliation: Antimicrobial Agents Unit, Faculty of science, University of Yaoundé I, P,O Box 812, Yaoundé, Cameroon. fabrice.boyom@fulbrightmail.org.

ABSTRACT

Background: Cancer has become a global public health problem and the search for new control measures is urgent. Investigation of plant products such as essential oils from Monodora myristica, Xylopia aethiopica and Xylopia parviflora might lead to new anticancer therapy. In this study, we have investigated the antineoplastic activity of essential oils from fruits of these plants growing in Chad and Cameroon.

Methods: The essential oils obtained by hydrodistillation of fruits of Monodora myristica, Xylopia aethiopica and Xylopia parviflora collected in Chad and Cameroon were analyzed by GC-FID and GC-MS and investigated for their antiproliferative activity against the breast cancer cell line (MCF7).

Results: Overall, monoterpenes were mostly found in the six essential oils. Oils from X. aethiopica and X. parviflora from Chad and Cameroon mainly contain β-pinene at 24.6%, 28.2%, 35.7% and 32.9% respectively. Monodora myristica oils from both origins contain mainly α-phellandrene at 52.7% and 67.1% respectively. The plant origin did not significantly influence the chemical composition of oils. The six essential oils exerted cytotoxic activity against cancer (MCF-7) and normal cell lines (ARPE-19), with more pronounced effect on neoplastic cells in the majority of cases. The highest selectivity was obtained with the essential oils of X. parviflora from Chad and Cameroon (5.87 and 5.54) which were more cytotoxic against MCF-7 than against normal cell line (ARPE-19) with IC50 values of 0.155 μL/mL and 0.166 μL/mL respectively.

Conclusions: Essential oils from fruits of Monodora myristica, Xylopia aethiopica and Xylopia parviflora have shown acceptable antineoplastic potency, and might be investigated further in this regard.

Show MeSH
Related in: MedlinePlus