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Efficacy of a mesenchymal stem cell loaded surgical mesh for tendon repair in rats.

Schon LC, Gill N, Thorpe M, Davis J, Nadaud J, Kim J, Molligan J, Zhang Z - J Transl Med (2014)

Bottom Line: The MSC-loaded mesh composed of a piece of conventional surgical mesh and a layer of scaffold, which supported MSC-embedded alginate gel.A 3-mm defect was surgically created at the Achilles tendon-gastrocnemius/soleus junction in 30 rats.Application of the MSC-loaded mesh, as a new device and MSC delivery vehicle, may benefit to early functional recovery of the ruptured tendon.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Orthopaedic Surgery, MedStar Union Memorial Hospital, 3333 North Calvert Street, Johnston Professional Building, Suite 400, Baltimore, MD 21218, USA. lewschon@gmail.com.

ABSTRACT

Objectives: The purpose of this study was to investigate the efficacy of a composite surgical mesh for delivery of mesenchymal stem cells (MSCs) in tendon repair.

Methods: The MSC-loaded mesh composed of a piece of conventional surgical mesh and a layer of scaffold, which supported MSC-embedded alginate gel. A 3-mm defect was surgically created at the Achilles tendon-gastrocnemius/soleus junction in 30 rats. The tendon defects were repaired with either 1) MSC-loaded mesh; or 2) surgical mesh only; or 3) routine surgical suture. Repaired tendons were harvested at days 6 and 14 for histology, which was scored on the bases of collagen organization, vascularity and cellularity, and immunohistochemisty of types I and III collagen.

Results: In comparison with the other two repair types, at day 6, the MSC-loaded mesh significantly improved the quality of the repaired tendons with dense and parallel collagen bundles, reduced vascularity and increased type I collagen. At day 14, the MSC-loaded mesh repaired tendons had better collagen formation and organization.

Conclusion: The MSC-loaded mesh enhanced early tendon healing, particularly the quality of collagen bundles. Application of the MSC-loaded mesh, as a new device and MSC delivery vehicle, may benefit to early functional recovery of the ruptured tendon.

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Quantification of immunohistochemistry of types I and III collagen in tendon repair. The staining of types I and III collagen was quantified in 1) staining area (A and C) and 2) staining intensity (B and D). In M + S group, the area of type I collagen was significantly increased from day 6 to day 14. The staining area of type III collagen was indifferent among the three groups. The area ratio of type III collagen over type I collagen in M + S group was greater than in the Sut group at day 6 (E). No differences were detected in average intensity of types I and III collagen, individually and the ratio (F), among the three groups.
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Figure 5: Quantification of immunohistochemistry of types I and III collagen in tendon repair. The staining of types I and III collagen was quantified in 1) staining area (A and C) and 2) staining intensity (B and D). In M + S group, the area of type I collagen was significantly increased from day 6 to day 14. The staining area of type III collagen was indifferent among the three groups. The area ratio of type III collagen over type I collagen in M + S group was greater than in the Sut group at day 6 (E). No differences were detected in average intensity of types I and III collagen, individually and the ratio (F), among the three groups.

Mentions: Immunohistochemistry demonstrated similar distribution patterns of types I and III collagen among the three groups (Figure 4). At the same time point, type I collagen staining area and intensity were not statistically different among the three groups in (Figure 5A and B). The area of type I collagen staining increased from day 6 to day 14 in the M + S groups (p < 0.05), but this was not in M and Sut groups.


Efficacy of a mesenchymal stem cell loaded surgical mesh for tendon repair in rats.

Schon LC, Gill N, Thorpe M, Davis J, Nadaud J, Kim J, Molligan J, Zhang Z - J Transl Med (2014)

Quantification of immunohistochemistry of types I and III collagen in tendon repair. The staining of types I and III collagen was quantified in 1) staining area (A and C) and 2) staining intensity (B and D). In M + S group, the area of type I collagen was significantly increased from day 6 to day 14. The staining area of type III collagen was indifferent among the three groups. The area ratio of type III collagen over type I collagen in M + S group was greater than in the Sut group at day 6 (E). No differences were detected in average intensity of types I and III collagen, individually and the ratio (F), among the three groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4020316&req=5

Figure 5: Quantification of immunohistochemistry of types I and III collagen in tendon repair. The staining of types I and III collagen was quantified in 1) staining area (A and C) and 2) staining intensity (B and D). In M + S group, the area of type I collagen was significantly increased from day 6 to day 14. The staining area of type III collagen was indifferent among the three groups. The area ratio of type III collagen over type I collagen in M + S group was greater than in the Sut group at day 6 (E). No differences were detected in average intensity of types I and III collagen, individually and the ratio (F), among the three groups.
Mentions: Immunohistochemistry demonstrated similar distribution patterns of types I and III collagen among the three groups (Figure 4). At the same time point, type I collagen staining area and intensity were not statistically different among the three groups in (Figure 5A and B). The area of type I collagen staining increased from day 6 to day 14 in the M + S groups (p < 0.05), but this was not in M and Sut groups.

Bottom Line: The MSC-loaded mesh composed of a piece of conventional surgical mesh and a layer of scaffold, which supported MSC-embedded alginate gel.A 3-mm defect was surgically created at the Achilles tendon-gastrocnemius/soleus junction in 30 rats.Application of the MSC-loaded mesh, as a new device and MSC delivery vehicle, may benefit to early functional recovery of the ruptured tendon.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Orthopaedic Surgery, MedStar Union Memorial Hospital, 3333 North Calvert Street, Johnston Professional Building, Suite 400, Baltimore, MD 21218, USA. lewschon@gmail.com.

ABSTRACT

Objectives: The purpose of this study was to investigate the efficacy of a composite surgical mesh for delivery of mesenchymal stem cells (MSCs) in tendon repair.

Methods: The MSC-loaded mesh composed of a piece of conventional surgical mesh and a layer of scaffold, which supported MSC-embedded alginate gel. A 3-mm defect was surgically created at the Achilles tendon-gastrocnemius/soleus junction in 30 rats. The tendon defects were repaired with either 1) MSC-loaded mesh; or 2) surgical mesh only; or 3) routine surgical suture. Repaired tendons were harvested at days 6 and 14 for histology, which was scored on the bases of collagen organization, vascularity and cellularity, and immunohistochemisty of types I and III collagen.

Results: In comparison with the other two repair types, at day 6, the MSC-loaded mesh significantly improved the quality of the repaired tendons with dense and parallel collagen bundles, reduced vascularity and increased type I collagen. At day 14, the MSC-loaded mesh repaired tendons had better collagen formation and organization.

Conclusion: The MSC-loaded mesh enhanced early tendon healing, particularly the quality of collagen bundles. Application of the MSC-loaded mesh, as a new device and MSC delivery vehicle, may benefit to early functional recovery of the ruptured tendon.

Show MeSH
Related in: MedlinePlus